Jason E King

US Patent:

20230065419, Mar 2, 2023

Filed:

Dec 16, 2020

Appl. No.:

17/785820

Inventors:

- Boston MA, US
Jason King - Boston MA, US
Huey-Ming Mak - Boston MA, US
Gabriel Rodriguez - Boston MA, US
Emily E. Wrenbeck - Boston MA, US
David Young - Boston MA, US

Assignee:

Ginkgo Bioworks, Inc. - Boston MA

International Classification:

C12P 19/34
C12N 15/113
C12P 13/24
C12N 15/52
C12N 15/70

Abstract:

Aspects of the disclosure relate to biosynthesis of histidine in host cells. For example, host cells may comprise: a promoter; a ribosome binding site (RBS); and a nucleic acid comprising: hisG; hisD; hisC hisB; hisH; hisA; hisF; and/or hisI. Host cells may further comprise a nucleic acid encoding a ribose phosphate pyrophosphokinase (RPPK), optionally comprising one or more amino acid substitutions relative to the sequence of wildtype RPPK. Host cells of the disclosure may comprise a nucleic acid encoding a 5,10-methylene-tetrahydrofolate dehydrogenase/5,10-methylene-tetrahydrofolate cyclohydrolase (MTHFDC) enzyme. Further aspects of the disclosure relate to production of purine pathway metabolites and/or plasmid DNA in host cells.