Jason M Meyers

US Patent:

20080021205, Jan 24, 2008

Filed:

Dec 10, 2004

Appl. No.:

10/581503

Inventors:

Helen Blau - Menlo Park CA, US
George Sen - Stanford CA, US
Jason Meyers - Menlo Park CA, US
Tom Wehrman - Fremont CA, US

International Classification:

C07H 19/00
C07H 21/02

US Classification:

536 231, 435183, 536 221, 536 253

Abstract:

Methods and compositions for producing hRNA, e.g., shRNA, expression modules for specific target nucleic acids are provided. In the subject methods, an initial nucleic acid, e.g., dsDNA, synthetic DNA, etc., corresponding to the target nucleic acid of interest is converted to an intermediate nucleic acid. The resultant intermediate nucleic acid, following an optional size modification step, is then converted to a linear dsDNA that includes at least one copy of the hRNA expression module of interest, or a precursor (i.e., pro-shRNA expression module) thereof, where in certain embodiments conversion may include amplification. Also provided are reagents, systems and kits for use in practicing the subject methods. The subject methods and compositions find use in a variety of different applications.