Jason R Spence

Address:

605 Sunset Rd, Ann Arbor, MI 48103

VIN:

3VWEF71K77M156599

Make:

VOLKSWAGEN

Model:

JETTA

Year:

2007

US Patent:

20130137130, May 30, 2013

Filed:

May 6, 2011

Appl. No.:

13/695887

Inventors:

James M. Wells - Cincinnati OH, US
Jason R. Spence - Cincinnati OH, US
Aaron M. Zorn - Cincinnati OH, US
Noah F. Shroyer - Cincinnati OH, US

Assignee:

CHILDREN'S HOSPITAL MEDICAL CENTER - Cincinnati OH

International Classification:

C12N 5/071

US Classification:

435 29, 435377

Abstract:

The generation of complex organ tissues from human embryonic and pluripotent stem cells (PSCs) remains a major challenge for translational studies. It is shown that PSCs can be directed to differentiate into intestinal tissue in vitro by modulating the combinatorial activities of several signaling pathways in a step-wise fashion, effectively recapitulating in vivo fetal intestinal al development. The resulting intestinal “organoids” were three-dimensional structures consisting of a polarized, columnar epithelium surrounded by mesenchyme that included a smooth muscle-like layer. The epithelium was patterned into crypt-like SOX9-positive proliferative zones and villus-like structures with all of the major functional cell types of the intestine. The culture system is used to demonstrate that expression of NEUROG3, a pro-endocrine transcription factor mutated in enteric anendocrinosis is sufficient to promote differentiation towards the enteroendocrine cell lineage. In conclusion, PSC-derived human intestinal tissue should allow for unprecedented studies of human intestinal development, homeostasis and disease.

US Patent:

20220195395, Jun 23, 2022

Filed:

Dec 13, 2021

Appl. No.:

17/549028

Inventors:

- Ann Arbor MI, US
Emily Holloway - Ann Arbor MI, US
Jason Spence - Ann Arbor MI, US

International Classification:

C12N 5/071
G01N 33/50

Abstract:

The invention disclosed herein generally relates to methods and systems for improving physiological growth of cultured tissues. In particular, the invention disclosed herein relates to methods and systems for promoting maintenance of cultured intestinal organoids (e.g., derived from pluripotent stem cells or from primary sources such as biopsy tissue).

US Patent:

20210371815, Dec 2, 2021

Filed:

May 27, 2021

Appl. No.:

17/332532

Inventors:

- Ann Arbor MI, US
Jason Spence - Ann Arbor MI, US

International Classification:

C12N 5/071

Abstract:

The invention disclosed herein generally relates to methods and systems for converting stem cells into specific tissue(s) or organ(s) through directed differentiation. In particular, the invention disclosed herein relates to methods and systems for promoting vascularized human intestinal organoid tissue having an intestine-specific EC transcriptional signature from hindgut spheroid tissue produced in vitro from the described methods.

US Patent:

20200190478, Jun 18, 2020

Filed:

Oct 11, 2019

Appl. No.:

16/599620

Inventors:

- Cincinnati OH, US
Aaron M. Zorn - Cincinnati OH, US
Jason R. Spence - Ann Arbor MI, US
Noah F. Shroyer - Houston TX, US

International Classification:

C12N 5/071
C12N 5/077

Abstract:

The generation of complex organ tissues from human embryonic and pluripotent stem cells (PSCs) remains a major challenge for translational studies. It is shown that PSCs can be directed to differentiate into intestinal tissue in vitro by modulating the combinatorial activities of several signaling pathways in a step-wise fashion, effectively recapitulating in vivo fetal intestinal development. The resulting intestinal “organoids” were three-dimensional structures consisting of a polarized, columnar epithelium surrounded by mesenchyme that included a smooth muscle-like layer. The epithelium was patterned into crypt-like SOX9-positive proliferative zones and villus-like structures with all of the major functional cell types of the intestine. The culture system is used to demonstrate that expression of NEUROG3, a pro-endocrine transcription factor mutated in enteric anendocrinosis is sufficient to promote differentiation towards the enteroendocrine cell lineage. In conclusion, PSC-derived human intestinal tissue should allow for unprecedented studies of human intestinal development, homeostasis and disease.

US Patent:

20200078493, Mar 12, 2020

Filed:

Mar 9, 2018

Appl. No.:

16/492263

Inventors:

- Atlanta GA, US
- Ann Arbor MI, US
Asma NUSRAT - Ann Arbor MI, US
Jason R. SPENCE - Ann Arbor MI, US
Miguel QUIROS - Ann Arbor MI, US

International Classification:

A61L 27/52
A61K 35/50
A61K 35/545
A61K 35/28
A61K 35/38
A61L 27/38

Abstract:

Disclosed herein are synthetic hydrogel useful for the generation, storage and administration of cellular structures such as spheroids and organoids.

US Patent:

20170362573, Dec 21, 2017

Filed:

Jun 20, 2017

Appl. No.:

15/627588

Inventors:

- Cincinnati OH, US
Aaron M. Zorn - Cincinnati OH, US
Jason R. Spence - Ann Arbor MI, US
Noah F. Shroyer - Houston TX, US

International Classification:

C12N 5/071
C12N 5/077

Abstract:

The generation of complex organ tissues from human embryonic and pluripotent stem cells (PSCs) remains a major challenge for translational studies. It is shown that PSCs can be directed to differentiate into intestinal tissue in vitro by modulating the combinatorial activities of several signaling pathways in a step-wise fashion, effectively recapitulating in vivo fetal intestinal development. The resulting intestinal “organoids” were three-dimensional structures consisting of a polarized, columnar epithelium surrounded by mesenchyme that included a smooth muscle-like layer. The epithelium was patterned into crypt-like SOX9-positive proliferative zones and villus-like structures with all of the major functional cell types of the intestine. The culture system is used to demonstrate that expression of NEUROG3, a pro-endocrine transcription factor mutated in enteric anendocrinosis is sufficient to promote differentiation towards the enteroendocrine cell lineage. In conclusion, PSC-derived human intestinal tissue should allow for unprecedented studies of human intestinal development, homeostasis and disease.