Jeffrey Keith Gentry

US Patent:

20210139837, May 13, 2021

Filed:

Nov 11, 2020

Appl. No.:

17/095028

Inventors:

- Germantown MD, US
Bryan BUTMAN - Germantown MD, US
Justin INSLEE - Boulder CO, US
Robert SCHNEIDER - Boulder CO, US
Kraig KRUGER - Boulder CO, US
Travis ANDREWS - Boulder CO, US
William HOCK - Boulder CO, US
David CERRONE - Boulder CO, US
Scott SCHMIDT - Boulder CO, US
Jeffrey GENTRY - Boulder CO, US
Mackenzie MILLER - Boulder CO, US
Vincent SO - Campbell CA, US
Chad B. GREEN - Campbell CA, US

Assignee:

Precigen, Inc. - Germantown MD

International Classification:

C12M 1/42
C12M 1/26
C12M 1/00

Abstract:

Electroporation is a technique in which an electrical field is applied to cells in order to increase the permeability of the cell membrane. This allows for chemicals, drugs, and/or macromolecules such as proteins and nucleic acids to be introduced into the cells. Embodiments relate to an electroporation apparatus. The electroporation apparatus comprises: a plurality of chambers configured to store a plurality of cells during an electroporation process; a plurality of electrodes configured to generate a plurality of electric fields within the plurality of chambers during the electroporation process, each electric field of the plurality of electric fields corresponding to one chamber of the plurality of chambers; a flow channel configured to transport the plurality cells during a cell collection process after the electroporation process; and a plurality of valves connecting the plurality of chambers to the flow channel.