Jeffrey Lin

US Patent:

8481281, Jul 9, 2013

Filed:

Feb 17, 2011

Appl. No.:

13/029830

Inventors:

Plamen A. Demirev - Ellicott City MD, US
Nathan A. Hagan - Ellicott City MD, US
Miquel D. Antoine - Columbia MD, US
Jeffrey S. Lin - Silver Spring MD, US
Andrew B. Feldman - Columbia MD, US

Assignee:

The Johns Hopkins University - Baltimore MD

International Classification:

C12Q 1/04
C12Q 1/18
C12Q 1/10
C12Q 1/14

US Classification:

435 34, 435 32, 435 36, 435 38

Abstract:

The present invention is based on the discovery that drug resistance in microorganisms can be rapidly and accurately determined using mass spectrometry. A mass spectrum of an intact microorganism or one or more isolated biomarkers from the microorganism grown in drug containing, isotopically-labeled media is compared with a mass spectrum of the intact microorganism or one or more isolated biomarkers from the microorganism grown in non-labeled media without the drug present. Drug resistance is determined by predicting and detecting a characteristic mass shift of one or more biomarkers using algorithms. The characteristic mass shift is indicative that the microorganism is growing in the presence of the drug and incorporating the isotopic label into the one or more biomarkers, resulting in change in mass.