This application is the expression and purification of a recombinant (3D7) MSP-1. The method of the present invention produces a highly purified protein which retains folding and disulfide bridging of the native molecule. The recombinant MSP-1is useful as a diagnostic reagent, for use in antibody production, and as a vaccine.
In this application is the expression and purification of a recombinant (3D7) MSP-1. The method of the present invention produces a highly purified protein which retains folding and disulfide bridging of the native molecule. The recombinant MSP-1is useful as a diagnostic reagent, for use in antibody production, and as a vaccine.
Expression, Purification And Uses Of A Liver Stage Antigen 1 Polypeptide
David E. Lanar - Takoma Park MD, US Collette J. Hillier - Concord, AU Jeffrey A. Lyon - Accident MD, US Evelina Angov - Bethesda MD, US Sanjai Kumar - Gaithersburg MD, US William Rogers - Jakata, ID Arnoldo Barbosa - Tolima, CO
Assignee:
The United States of America as represented by the Secretary of the Navy - Washington DC
International Classification:
C12N 15/00 A61K 39/015 C07K 14/445
US Classification:
435 691, 435 71, 4241911, 530350
Abstract:
In this application is described the expression and purification of a recombinant (3D7) LSA-NRC polypeptide. The method of the present invention produces a highly purified polypeptide which is useful as a vaccine and as a diagnostic reagent.
Isolation And Purification Of Merozoite Protein-142 Vaccine
In this application is the expression and purification of a recombinant (3D7) MSP-1. The method of the present invention produces a highly purified protein which retains folding and disulfide bridging of the native molecule. The recombinant MSP-1is useful as a diagnostic reagent, for use in antibody production, and as a vaccine.
Expression, Purification And Uses Of A Plasmodium Falciparum Liver Stage Antigen 1 Polypeptide
David E. Lanar - Takoma Park MD, US Collette J. Hillier - Concord, AU Jeffrey A. Lyon - Accident MD, US Evelina Angov - Bethesda MD, US Sanjai Kumar - Gaithersburg MD, US William Rogers - FPO AP, US Arnoldo Barbosa - Tolima, CO
Assignee:
The United States of America as Represented by the Secretary of the Army - Washington DC
International Classification:
C12N 15/00 A61K 39/002
US Classification:
4353201, 4241911
Abstract:
In this application is described the expression and purification of a recombinant (3D7) LSA-NRC polypeptide. The method of the present invention produces a highly purified polypeptide which is useful as a vaccine and as a diagnostic reagent.
Isolation And Purification Of Merozoite Protein-1Vaccine
Jeffrey A. Lyon - Silver Spring MD, US Evelina Angov - Bethesda MD, US
Assignee:
The United States of America as Represented by the Secretary of the Army - Washington DC
International Classification:
A61K 39/00 A61K 39/002 A61K 39/015
US Classification:
4241851, 4241911, 4242651, 4242681
Abstract:
In this application is the expression and purification of a recombinant (3D7) MSP-1. The method of the present invention produces a highly purified protein which retains folding and disulfide bridging of the native molecule. The recombinant MSP-1is useful as a diagnostic reagent, for use in antibody production, and as a vaccine.
Protein Expression By Codon Harmonization And Translational Attenuation
The present invention is based in the fields of molecular genetics and recombinant protein expression. The invention features compositions and methods for increasing the expression levels of proteins expressed in non-natural hosts. Increased expression levels are achieved by expression of a translationally-harmonized nucleic acid that is created by substituting certain codons having low or intermediate usage frequency in the natural host with synonymous codons that have low or intermediate usage frequency in the non-natural host.
Computer-Driven Amino Acid Indexer For Peptide Synthesis
Stephen A. Van Albert - Silver Spring MD Jaime M. Lee - Silver Spring MD Jeffrey A. Lyon - Silver Spring MD John M. Carter - Gaitherburg MD
Assignee:
The United States of America as represented by the Secretary of the Army - Washington DC
International Classification:
G06F 1546 C12N 1500
US Classification:
364500
Abstract:
An automated, computer-driven amino acid indexer for peptide synthesis uses programmed computer, a circuit board controller, and a combination of microtiter sample well trays, light emitting diodes to illuminate each sample well, and circuitry to control the illumination of the diodes. The apparatus simplifies technical difficulties present in large-scale laboratory syntheses of peptides by substantially reducing the time required for dispensing amino acids into sample trays and reducing the occurrence of error in the process to negligible levels in typical syntheses. A programmed, automated technique for synthesizing peptides is also provided.