Skills:
Cell Biology: Flow cytometry, mammalian and bacterial cell culture, ELISA, cell binding assays Molecular Biology: PCR, Blotting techniques, DNA/RNA isolation techniques, RT-PCR Histology: IHC, microscopy, basic histological staining (H&E), fluorescence microscopy Animal Handling: Injection techniques (iv, ip, sc), husbandry, dosing (gavage), model development (xenograft, acute and chronic inflammatory disease models, transgenic models), breeding, basic surgical techniques, dissection and processing of tissue samples, isolation and labeling of atherosclerotic plaques in ApoE -/- mice, pharmacokinetics. Small Animal Imaging: FMT, IVIS, micro CT. Managerial: IACUC adminstration and protocol writing, compliance, animal facility management, biological safety and occupational health officer. Computer: Mac/PC, Microsoft Office, SoftMax Pro, Image Analysis Software (TruQuant, Living Image, Volocity).