Jeffrey K Nelson

US Patent:

6664388, Dec 16, 2003

Filed:

Mar 4, 2002

Appl. No.:

10/091231

Inventors:

Jeffrey S. Nelson - Woodinville WA

Assignee:

Applera Corporation - Foster City CA

International Classification:

C07H 2104

US Classification:

536 2531, 536 2532, 536 2533, 536 2534, 536 254, 536 273, 536 281, 536 291

Abstract:

The present invention provides a process for the removal of protecting groups, i. e. deprotection, from chemically synthesized oligonucleotides. In one embodiment, the invention provides reagents suitable for use in such a process, and kits incorporating such reagents in a convenient, ready-to-use format. By use of the process and reagents of the invention, side-reactions leading to certain impurities that contaminate the synthesized oligonucleotides can be minimized. Methods and reagents are provided for deprotection of an oligonucleotide by reacting a protected oligonucleotide with a deprotection reagent wherein the deprotection reagent comprises an active methylene compound and an amine reagent. The active methylene compound has the structure: where substituent EWG is an electron-withdrawing group and R is hydrogen, C -C alkyl, C -C aryl, heterocycle or an electron-withdrawing group.

US Patent:

20040191808, Sep 30, 2004

Filed:

Dec 16, 2003

Appl. No.:

10/737059

Inventors:

Jeffrey Nelson - Woodinville WA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

C12Q001/68
C07H021/04

US Classification:

435/006000, 536/025300

Abstract:

The present invention provides a process for the removal of protecting groups, i.e. deprotection, from chemically synthesized oligonucleotides. In one embodiment, the invention provides reagents suitable for use in such a process, and kits incorporating such reagents in a convenient, ready-to-use format. By use of the process and reagents of the invention, side-reactions leading to certain impurities that contaminate the synthesized oligonucleotides can be minimized. Methods and reagents are provided for deprotection of an oligonucleotide by reacting a protected oligonucleotide with a deprotection reagent wherein the deprotection reagent comprises an active methylene compound and an amine reagent. The active methylene compound has the structure: where substituent EWG is an electron-withdrawing group and R is hydrogen, C-Calkyl, C-Caryl, heterocycle or an electron-withdrawing group.

US Patent:

20050069928, Mar 31, 2005

Filed:

Aug 3, 2004

Appl. No.:

10/911239

Inventors:

Jeffrey Nelson - Woodinville WA, US
John Mulligan - Seattle WA, US
John Tabone - Bothell WA, US

Assignee:

Blue Heron Biotechnology, Inc. - Bothell WA

International Classification:

C12Q001/68
C07H021/04
C12P019/34
A61K048/00

US Classification:

435006000, 514044000, 435091200, 536025300

Abstract:

Disclosed is a significantly improved synthetic method of producing a set of mutagenized progeny polynucleotides which contain at least one substituted codon encoding for each of the 20 naturally encoded amino acids or any selected subset thereof. This in turn, similarly provides a method for producing from a parental template polypeptide, a set of mutagenized progeny polypeptides in which all 20 naturally encoded amino acids is represented at each original amino acid position or any selected subset thereof. The methods described herein enable the synthesis of defined, complex mixtures of oligonucleotides, in instances where the incorporation of degenerate bases is impractical. These oligonucleotide mixtures are useful for a variety of applications such as recombination methods, site-saturation mutagenesis, or the like.