Jeffrey F Sampson

US Patent:

20030104428, Jun 5, 2003

Filed:

Jun 21, 2002

Appl. No.:

10/177062

Inventors:

Daniel Branton - Lexington MA, US
Stephen Laderman - Menlo Park CA, US
Zohar Yakhini - Zikhron Ya'acov, IL
Jeffrey Sampson - San Francisco CA, US
Hui Wang - Cambridge MA, US

Assignee:

President and Fellows of Harvard College - Cambridge MA

International Classification:

C12Q001/68
C07H021/04
C12P019/34

US Classification:

435/006000, 435/091200, 536/023100

Abstract:

To probe the characteristics of a target nucleic acid molecule or a population of target nucleic acid molecules, a local cross-sectional area, local charge, or local chemistry of a molecule is modified. The modified nucleic acid is contacted with a substrate that includes a detector that is responsive to the modification in the local cross-sectional area, local charge, or local chemistry of the nucleic acid molecule. The modified nucleic acid molecule traverses a defined and preferably molecular dimensioned volume on the substrate so that nucleotides of the modified nucleic acid molecule interact with the detector in sequential order, whereby data correlating with the cross-sectional area, local charge, or local chemistry of the nucleic acid molecule are obtained. The nucleic acid molecule may be modified in a number of locations along the molecule's length. Because the individual nucleotides of the nucleic acid molecule interact with the detector in sequential order, information regarding the location and composition of a plurality of modified sites along a single molecule can be obtained.

US Patent:

20210079389, Mar 18, 2021

Filed:

Nov 24, 2020

Appl. No.:

17/103678

Inventors:

- Santa Clara CA, US
Douglas J. DELLINGER - Boulder CO, US
Jeffrey R. SAMPSON - San Jose CA, US
Robert KAISER - Santa Clara CA, US
Joel MYERSON - Berkeley CA, US

International Classification:

C12N 15/11
C12N 9/22
C07H 21/02
C12N 15/113
C12N 15/90
C12Q 1/6876

Abstract:

The present invention relates to modified guide RNAs and their use in clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems.

US Patent:

20180051281, Feb 22, 2018

Filed:

May 26, 2017

Appl. No.:

15/607295

Inventors:

- Santa Clara CA, US
Douglas J. Dellinger - Boulder CO, US
Jeffrey R. Sampson - San Jose CA, US
Robert Kaiser - Santa Clara CA, US
Joel Myerson - Berkeley CA, US

International Classification:

C12N 15/11
C12N 9/22
C12N 15/113
C12N 2310/20

Abstract:

The present invention relates to modified guide RNAs and their use in clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems.

US Patent:

20160289675, Oct 6, 2016

Filed:

Dec 3, 2015

Appl. No.:

14/757204

Inventors:

- Santa Clara CA, US
Douglas J. Dellinger - Boulder CO, US
Jeffrey R. Sampson - San Jose CA, US
Robert Kaiser - Santa Clara CA, US
Joel Myerson - Berkeley CA, US

International Classification:

C12N 15/113
C12N 15/90
C12Q 1/68

Abstract:

The present invention relates to modified guide RNAs and their use in clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems.