Jeffrey S Sampson

US Patent:

20100112556, May 6, 2010

Filed:

Nov 3, 2008

Appl. No.:

12/264091

Inventors:

JEFFREY R. SAMPSON - Loveland CO, US
Brian J. Peter - Loveland CO, US

International Classification:

C12Q 1/68

US Classification:

435 6

Abstract:

A method of sample analysis is provided. In certain embodiments, the method may comprise: a) contacting a plurality of Q probes with a nucleic acid sample comprising a target polynucleotide under hybridization conditions to form a plurality of flap endonuclease substrates each comprising a Q probe and a site in the target polynucleotide; b) contacting the plurality of flap endonuclease substrates with a flap endonuclease under cleavage conditions to produce cleavage products, in which each of the Q probes of the flap endonuclease substrates is cleaved to produce cleavage products that include at least a first fragment that is hybridized with a site in the target polynucleotide and a second fragment that is linear and free in solution; and c) detecting at least one of the cleavage products.

US Patent:

20130323725, Dec 5, 2013

Filed:

Mar 14, 2013

Appl. No.:

13/830408

Inventors:

ROBERT A. ACH - Loveland CO, US
ZOLTAN TIMAR - Loveland CO, US
JOEL MYERSON - Loveland CO, US
JEFFREY ROBERT SAMPSON - Loveland CO, US
HOLLY HOGREFE - Loveland CO, US

Assignee:

AGILENT TECHNOLOGIES, INC. - Loveland US

International Classification:

C12Q 1/68

US Classification:

435 611, 536 231

Abstract:

This disclosure provides a method comprising: a) clamping the top and bottom strands of a double stranded DNA molecule to produce a duplex in which the top and bottom strands are linked; b) denaturing the duplex to produce a denatured product; and c) renaturing the denatured product in the presence of a labeled oligonucleotide that is complementary to a sequence of nucleotides in the double stranded DNA molecule, thereby producing a D-loop-containing product. Kits for performing the method and products made by the method are also provided.

US Patent:

20210079389, Mar 18, 2021

Filed:

Nov 24, 2020

Appl. No.:

17/103678

Inventors:

- Santa Clara CA, US
Douglas J. DELLINGER - Boulder CO, US
Jeffrey R. SAMPSON - San Jose CA, US
Robert KAISER - Santa Clara CA, US
Joel MYERSON - Berkeley CA, US

International Classification:

C12N 15/11
C12N 9/22
C07H 21/02
C12N 15/113
C12N 15/90
C12Q 1/6876

Abstract:

The present invention relates to modified guide RNAs and their use in clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems.

US Patent:

20180051281, Feb 22, 2018

Filed:

May 26, 2017

Appl. No.:

15/607295

Inventors:

- Santa Clara CA, US
Douglas J. Dellinger - Boulder CO, US
Jeffrey R. Sampson - San Jose CA, US
Robert Kaiser - Santa Clara CA, US
Joel Myerson - Berkeley CA, US

International Classification:

C12N 15/11
C12N 9/22
C12N 15/113
C12N 2310/20

Abstract:

The present invention relates to modified guide RNAs and their use in clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems.

US Patent:

20160289675, Oct 6, 2016

Filed:

Dec 3, 2015

Appl. No.:

14/757204

Inventors:

- Santa Clara CA, US
Douglas J. Dellinger - Boulder CO, US
Jeffrey R. Sampson - San Jose CA, US
Robert Kaiser - Santa Clara CA, US
Joel Myerson - Berkeley CA, US

International Classification:

C12N 15/113
C12N 15/90
C12Q 1/68

Abstract:

The present invention relates to modified guide RNAs and their use in clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems.