Jeffrey T Voss

US Patent:

6783756, Aug 31, 2004

Filed:

Mar 30, 1999

Appl. No.:

09/281674

Inventors:

Hermann Bujard - Heidelberg, DE
Manfred Gossen - El Cerrito CA
Jochen G. Salfeld - North Grafton MA
Jeffrey W. Voss - West Boylston MA

Assignee:

Abbott GmbH Co., KG - Wiesbaden

International Classification:

A61K 6300

US Classification:

424 9321, 514 44, 435455, 435465

Abstract:

A method for regulating expression of a tet operator-linked gene in a cell of a subject is disclosed. In one embodiment, the method involves introducing into the cell a nucleic acid molecule encoding a tetracycline-controllable transactivator (tTA), the tTA comprising a Tet repressor operably linked to a polypeptide which directly or indirectly activates transcription in eucaryotic cells; and modulating the concentration of a tetracycline, or analogue thereof, in the subject. Alternatively, in another embodiment, the method involves obtaining the cell from the subject, introducing into the cell a first nucleic acid molecule which operatively links a gene to at least one tet operator sequence, introducing into the cell a second nucleic acid molecule encoding a tTA, to form a modified cell, administering the modified cell to the subject, and modulating the concentration of a tetracycline, or analogue thereof, in the subject. The first and second nucleic acid molecule can be within a single molecule (e. g. , in the same vector) or on separate molecules.

US Patent:

20020086426, Jul 4, 2002

Filed:

Feb 5, 2001

Appl. No.:

09/777317

Inventors:

Hermann Bujard - Heidelberg, DE
Manfred Gossen - El Cerrito CA, US
Jochen Salfeld - North Grafton MA, US
Jeffrey Voss - West Boylston MA, US

Assignee:

BASF Aktiengesellschaft - Ludwigshafen

International Classification:

C12N015/00

US Classification:

435/440000

Abstract:

A method for regulating expression of a tet operator-linked gene in a cell of a subject is disclosed. In one embodiment, the method involves introducing into the cell a nucleic acid molecule encoding a tetracycline-controllable transactivator (tTA), the tTA comprising a Tet repressor operably linked to a polypeptide which directly or indirectly activates transcription in eucaryotic cells; and modulating the concentration of a tetracycline, or analogue thereof, in the subject. Alternatively, in another embodiment, the method involves obtaining the cell from the subject, introducing into the cell a first nucleic acid molecule which operatively links a gene to at least one tet operator sequence, introducing into the cell a second nucleic acid molecule encoding a tTA, to form a modified cell, administering the modified cell to the subject, and modulating the concentration of a tetracycline, or analogue thereof, in the subject. The first and second nucleic acid molecule can be within a single molecule (e.g., in the same vector) or on separate molecules.

US Patent:

20020152487, Oct 17, 2002

Filed:

Jun 25, 2001

Appl. No.:

09/892227

Inventors:

Hermann Bujard - Heidelberg, DE
Manfred Gossen - El Cerrito CA, US
Jochen Salfeld - North Grafton MA, US
Jeffrey Voss - West Boylston MA, US

Assignee:

BASF Aktiengesellschaft

International Classification:

A01K067/027
A61K031/65

US Classification:

800/014000, 800/015000, 800/016000, 800/017000, 800/018000, 514/152000

Abstract:

Transgenic animals carrying two transgenes, the first coding for a transactivator fusion protein comprising a tet repressor and a polypeptide which directly or indirectly activates in eucaryotic cells, and the second comprising a gene operably linked to a minimal promotor operably linked to at least one tet operator sequence, are disclosed. Isolated DNA molecules (e.g., targeting vectors) for integrating a polynucleotide sequence encoding a transactivator of the invention at a predetermined location within a second target DNA molecule by homologous recombination are also disclosed. Transgenic animals having the DNA molecules of the invention integrated at a predetermined location in a chromosome by homologous recombination are also encompassed by the invention. Methods to regulate the expression of a tet operator linked-gene of interest by administering tetracycline or a tetracycline analogue to an animal of the invention are also disclosed. The regulatory system of the invention allows for conditional inactivation or modulation of expression of a gene of interest in a host cell or animal.

US Patent:

58889817, Mar 30, 1999

Filed:

Jun 7, 1995

Appl. No.:

8/479306

Inventors:

Hermann Bujard - Heidelberg, DE
Manfred Gossen - El Cerrito CA
Jochen G. Salfeld - North Grafton MA
Jeffrey W. Voss - West Boylston MA

Assignee:

BASF Aktiengesellschaft - Ludwigshafen
Knoll Aktiengesellschaft - Ludwigshafen

International Classification:

A61K 4800
C12N 1500

US Classification:

514 44

Abstract:

A method for regulating expression of a tet operator-linked gene in a cell of a subject is disclosed. In one embodiment, the method involves introducing into the cell a nucleic acid molecule encoding a tetracycline-controllable transactivator (tTA), the tTA comprising a Tet repressor operably linked to a polypeptide which directly or indirectly activates transcription in eucaryotic cells; and modulating the concentration of a tetracycline, or analogue thereof, in the subject. Alternatively, in another embodiment, the method involves obtaining the cell from the subject, introducing into the cell a first nucleic acid molecule which operatively links a gene to at least one tet operator sequence, introducing into the cell a second nucleic acid molecule encoding a tTA, to form a modified cell, administering the modified cell to the is subject, and modulating the concentration of a tetracycline, or analogue thereof, in the subject. The first and second nucleic acid molecule can be within a single molecule (e. g. , in the same vector) or on separate molecules.

US Patent:

62521365, Jun 26, 2001

Filed:

Sep 29, 1998

Appl. No.:

9/163269

Inventors:

Hermann Bujard - Heidelberg, DE
Manfred Gossen - El Cerrito CA
Jochen G. Salfeld - North Grafton MA
Jeffrey W. Voss - West Boylston MA

Assignee:

BASF Aktiengesellschaft
Knoll Aktiengesellschaft

International Classification:

C12N 1509
C12N 1582
C12N 1587
C12N 1590
A01H 500

US Classification:

800278

Abstract:

Transgenic animals carrying two transgenes, the first coding for a transactivator fusion protein comprising a tet repressor and a polypeptide which directly or indirectly activates in eucaryotic cells, and the second comprising a gene operably linked to a minimal promoter operably linked to at least one tet operator sequence, are disclosed. Isolated DNA molecules (e. g. , targeting vectors) for integrating a polynucleotide sequence encoding a transactivator of the invention at a predetermined location within a second target DNA molecule by homologous recombination are also disclosed. Transgenic animals having the DNA molecules of the invention integrated at a predetermined location in a chromosome by homologous recombination are also encompassed by the invention. Methods to regulate the expression of a tet operator linked-gene of interest by administering tetracycline or a tetracycline analogue to an animal of the invention are also disclosed. The regulatory system of the invention allows for conditional inactivation or modulation of expression of a gene of interest in a host cell or animal.

US Patent:

48588903, Aug 22, 1989

Filed:

Feb 26, 1988

Appl. No.:

7/160530

Inventors:

Ronald E. Fowler - Long Grove IL
Jeffrey L. Voss - Elgin IL
Stephen D. Cain - Canton GA

Assignee:

Brand Scaffold Services, Ltd. - Park Ridge IL

International Classification:

B66C 2330
B66D 112
B66D 320

US Classification:

254335

Abstract:

A hoist and method are provided for lifting objects inside a vessel having a wall defining a port and having a platform on the exterior of the wall. The hoist includes a frame with a support portion extending outwardly from the port adjacent the platform and a cantilevered portion for extending through, and inwardly from, the port. A suitable clamping system is provided for clamping the frame support portion to the platform. A hoist cable is provided along the frame from the support portion to the cantilevered portion and extends over a sheave at the end of the cantilevered portion so that it hangs down inside the vessel. A winch is operatively engaged with the hoist cable and is mounted to the frame support portion for feeding out and retracting the cable whereby the end of the cable may be raised and lowered inside the vessel.