Jennifer J Black

Address:

7511 S 169 St, Omaha, NE 68136

VIN:

2HKYF18457H521212

Make:

HONDA

Model:

PILOT

Year:

2007

US Patent:

7291480, Nov 6, 2007

Filed:

Sep 15, 2004

Appl. No.:

10/941132

Inventors:

Jennifer A. Black - Bennington NE, US
Kenneth S. Thomson - Omaha NE, US

International Classification:

C12Q 1/00
C12Q 1/02
C12Q 1/04
C12Q 1/18

US Classification:

435 29, 435 32, 435 35

Abstract:

A method for determining whether a microorganism produces an AmpC β-lactamase is disclosed in which a culture of a microorganism suspected of producing a β-lactamase that inactivates a β-lactam-containing antibiotic is admixed with an effective amount of each of i) a β-lactam-containing antibiotic, ii) a β-lactamase inhibitor to which AmpC β-lactamase is resistant, and iii) a permeabilizing agent for the microorganism present in a non-growth-inhibiting microorganism-permeabilizing amount to form an assay culture. That assay culture in maintained under appropriate culture conditions and for a time period sufficient to determine the interaction of the microorganism with the AmpC β-lactamase resistant inhibitor and antibacterial compound, and thereby determine the presence of an AmpC β-lactamase, wherein a positive test indicates the presence of an AmpC β-lactamase.

US Patent:

7335485, Feb 26, 2008

Filed:

Dec 8, 2006

Appl. No.:

11/636103

Inventors:

Jennifer A. Black - Omaha NE, US
Ellen S. Moland - Crescent IA, US
Kenneth Thomson - Omaha NE, US

Assignee:

Creighton University - Omaha NE

International Classification:

C12Q 1/00
C12Q 1/02
C12Q 1/18

US Classification:

435 29, 435 32, 435 34, 435 35

Abstract:

The present invention provides a method of determining the antibiotic susceptibility of a microorganism comprising the following steps. First, a culture of the microorganism whose susceptibility is to be determined is admixed with an antibiotic to which susceptibility is to be assayed, and a permeabilizing agent for the microorganism present in a non-growth-inhibiting microorganism-permeabilizing effective amount to form an assay culture. Next, the assay culture is incubated under appropriate culture conditions and for a time sufficient to determine the susceptibility of the microorganism to the antibiotic. In another aspect, the present invention provides an improved method for antibiotic susceptibility testing of a microorganism in a culture by admixing the culture with an antibiotic to which susceptibility is to be assayed, and incubating the culture for a time sufficient to determine the susceptibility of the microorganism to the antibiotic, the improvement comprising admixing the culture with a permeabilizing agent for the microorganism present in a non-growth inhibiting microorganism-permeabilizing effective amount.

US Patent:

7452691, Nov 18, 2008

Filed:

Mar 5, 2007

Appl. No.:

11/713884

Inventors:

Jennifer A. Black - Omaha NE, US
Ellen S. Moland - Crescent IA, US
Kenneth Thomson - Omaha NE, US

Assignee:

Creighton University - Omaha NE

International Classification:

C12Q 1/02
C12Q 1/04
C12Q 1/16

US Classification:

435 29, 435 32, 435 34, 435 35

Abstract:

The present invention provides a method of detecting production of antibiotic-inactivating factor and for determining the antibiotic susceptibility of a microorganism comprising the following steps, a culture of the microorganism suspected of producing inactivating factors and/or whose susceptibility is to be determined is admixed with an antibiotic to which susceptibility is to be assayed, and a permeabilizing agent for the microorganism present in a non-growth-inhibiting microorganism-permeabilizing effective amount to form an assay culture. The assay culture is incubated under appropriate culture conditions and for a time sufficient to determine production of antibiotic-inactivating factors and/or the susceptibility of the microorganism to the antibiotic. In another aspect, the present invention provides an improved method for antibiotic susceptibility testing of a microorganism in a culture by admixing the culture with an antibiotic to which susceptibility is to be assayed, and incubating the culture for a time sufficient to determine the susceptibility of the microorganism to the antibiotic, the improvement comprising admixing the culture with a permeabilizing agent for the microorganism present in a non-growth inhibiting microorganism-permeabilizing effective amount.

US Patent:

7718392, May 18, 2010

Filed:

Jul 24, 2007

Appl. No.:

11/880768

Inventors:

Jennifer A. Black - Omaha NE, US
Ellen S. Moland - Crescent IA, US
Kenneth Thomson - Omaha NE, US

Assignee:

Creighton University - Omaha NE

International Classification:

C12Q 1/00
C12Q 1/02
C12Q 1/04
C12Q 1/18

US Classification:

435 29, 435 32, 435 35

Abstract:

The present invention provides a method of determining the antibiotic susceptibility of a microorganism comprising the following steps. First, a culture of the microorganism whose susceptibility is to be determined is admixed with an antibiotic to which susceptibility is to be assayed, and a permeabilizing agent for the microorganism present in a non-growth-inhibiting microorganism-permeabilizing effective amount to form an assay culture. Next, the assay culture is incubated under appropriate culture conditions and for a time sufficient to determine the susceptibility of the microorganism to the antibiotic. In another aspect, the present invention provides an improved method for antibiotic susceptibility testing of a microorganism in a culture by admixing the culture with an antibiotic to which susceptibility is to be assayed, and incubating the culture for a time sufficient to determine the susceptibility of the microorganism to the antibiotic, the improvement comprising admixing the culture with a permeabilizing agent for the microorganism present in a non-growth inhibiting microorganism-permeabilizing effective amount.

US Patent:

7807403, Oct 5, 2010

Filed:

Sep 26, 2007

Appl. No.:

11/904254

Inventors:

Jennifer A. Black - Omaha NE, US
Kenneth S. Thomson - Omaha NE, US

Assignee:

Creighton University - Omaha NE

International Classification:

C12Q 1/00
C12Q 1/04
C12Q 1/18

US Classification:

435 29, 435 32, 435 34, 435 35

Abstract:

A method for determining whether a microorganism produces an AmpC β-lactamase is disclosed in which a culture of a microorganism suspected of producing a β-lactamase that inactivates a β-lactam-containing antibiotic is admixed with an effective amount of each of i) a β-lactam-containing antibiotic, ii) a β-lactamase inhibitor to which AmpC β-lactamase is resistant, and iii) a permeabilizing agent for the microorganism present in a non-growth-inhibiting microorganism-permeabilizing amount to form an assay culture. That assay culture in maintained under appropriate culture conditions and for a time period sufficient to determine the interaction of the microorganism with the AmpC β-lactamase resistant inhibitor and antibacterial compound, and thereby determine the presence of an AmpC β-lactamase, wherein a positive test indicates the presence of an AmpC β-lactamase.

US Patent:

7267962, Sep 11, 2007

Filed:

Jul 7, 2005

Appl. No.:

11/176420

Inventors:

Jennifer A. Black - Omaha NE, US
Ellen S. Moland - Crescent IA, US
Kenneth S. Thomson - Omaha NE, US

Assignee:

Creighton University - Omaha NE

International Classification:

C12Q 1/00
C12Q 1/02
C12Q 1/04
C12Q 1/18

US Classification:

435 29, 435 32, 435 35

Abstract:

The present invention provides a method of determining the antibiotic susceptibility of a microorganism comprising the following steps. First, a culture of the microorganism whose susceptibility is to be determined is admixed with an antibiotic to which susceptibility is to be assayed, and a permeabilizing agent for the microorganism present in a non-growth-inhibiting microorganism-permeabilizing effective amount to form an assay culture. Next, the assay culture is incubated under appropriate culture conditions and for a time sufficient to determine the susceptibility of the microorganism to the antibiotic. In another aspect, the present invention provides an improved method for antibiotic susceptibility testing of a microorganism in a culture by admixing the culture with an antibiotic to which susceptibility is to be assayed, and incubating the culture for a time sufficient to determine the susceptibility of the microorganism to the antibiotic, the improvement comprising admixing the culture with a permeabilizing agent for the microorganism present in a non-growth inhibiting microorganism-permeabilizing effective amount.

US Patent:

20040115756, Jun 17, 2004

Filed:

Mar 13, 2003

Appl. No.:

10/387788

Inventors:

Jennifer Black - Omaha NE, US
Ellen A. Moland - Crescent IA, US
Kenneth Thomson - Omaha NE, US

International Classification:

C12Q001/18

US Classification:

435/032000

Abstract:

The present invention provides a method of determining the antibiotic susceptibility of a microorganism comprising the following steps. First, a culture of the microorganism whose susceptibility is to be determined is admixed with an antibiotic to which susceptibility is to be assayed, and a permeabilizing agent for the microorganism present in a non-growth-inhibiting microorganism-permeabilizing effective amount to form an assay culture. Next, the assay culture is incubated under appropriate culture conditions and for a time sufficient to determine the susceptibility of the microorganism to the antibiotic. In another aspect, the present invention provides an improved method for antibiotic susceptibility testing of a microorganism in a culture by admixing the culture with an antibiotic to which susceptibility is to be assayed, and incubating the culture for a time sufficient to determine the susceptibility of the microorganism to the antibiotic, the improvement comprising admixing the culture with a permeabilizing agent for the microorganism present in a non-growth inhibiting microorganism-permeabilizing effective amount.

US Patent:

20050244917, Nov 3, 2005

Filed:

Jul 8, 2005

Appl. No.:

11/177714

Inventors:

Jennifer Black - Omaha NE, US
Ellen Moland - Crescent IA, US
Kenneth Thomson - Omaha NE, US

International Classification:

C12Q001/18

US Classification:

435032000

Abstract:

The present invention provides a method of determining the antibiotic susceptibility of a microorganism comprising the following steps. First, a culture of the microorganism whose susceptibility is to be determined is admixed with an antibiotic to which susceptibility is to be assayed, and a permeabilizing agent for the microorganism present in a non-growth-inhibiting microorganism-permeabilizing effective amount to form an assay culture. Next, the assay culture is incubated under appropriate culture conditions and for a time sufficient to determine the susceptibility of the microorganism to the antibiotic. In another aspect, the present invention provides an improved method for antibiotic susceptibility testing of a microorganism in a culture by admixing the culture with an antibiotic to which susceptibility is to be assayed, and incubating the culture for a time sufficient to determine the susceptibility of the microorganism to the antibiotic, the improvement comprising admixing the culture with a permeabilizing agent for the microorganism present in a non-growth inhibiting microorganism-permeabilizing effective amount.