University of Utah Research Foundation - Salt Lake City UT
International Classification:
G01N 33/53
US Classification:
435 61, 4352872
Abstract:
Methods, assays, and products for the detection of analytes in a sample are provided. In one aspect, for example, a device for detecting an analyte in a sample can include a fluid transfer membrane further including a sample input region operable to receive a liquid sample, a reagent region including a first split aptamer segment, a second split aptamer segment, and a detection marker, where the first and second split aptamers are operable to ligate in the presence of the analyte. The detection marker is operable to bind to the second split aptamer. The device can further include a test region having an immobilized binding reagent operable to bind to the first split aptamer segment such that the detection marker is held in the test region when the first split aptamer segment is ligated to the second split aptamer segment due to the analyte being present in the sample.
Methods, assays, and products for the detection of small molecules are provided. In one aspect, for example, a method of detecting a small molecule in a sample can include reacting together a first half of a DNA split aptamer having a first reactive group coupled thereto, a second half of a DNA split aptamer having a second reactive group coupled thereto, where the DNA split aptamer is selective for the small molecule, and a sample containing the small molecule. The first half and the second half bind to the small molecule and the first reactive group and the second reactive group react to form an aptamer ligation product of the first half and the second half. The method can also include assaying for the aptamer ligation product in order to detect the small molecule presence in the sample.
Methods Of Identifying Adenosine-To-Inosine Edited Rna
- Atlanta GA, US Jennifer M. Heemstra - Atlanta GA, US
International Classification:
C12N 15/10 C12Q 1/6806 C12N 9/22
Abstract:
This disclosure relates to improved methods of identifying A-to-I RNA edits in a sample. In certain embodiments, this disclosure relates to methods of purifying RNA containing an inosine base comprising the steps of: exposing an RNA sample to endonuclease V or fusion thereof and calcium ions in the absence of magnesium ions providing an RNA and endonuclease V binding complex. In certain embodiments, the methods further comprise purifying the RNA and endonuclease V binding complex from unbound RNA in the sample; separating the RNA from endonuclease V providing separated RNA; sequencing the separated RNA; and identifying positions in the RNA sequences wherein A-to-I edits occur. In certain embodiments, the RNA is derived from a cell.
Methods Of Imaging And Tracking Nucleic Acids In Cells
- Atlanta GA, US Jennifer Heemstra - Atlanta GA, US
International Classification:
C12N 15/115
Abstract:
This disclosure relates to methods of imaging and tracking RNA or other nucleic acids in cells. In certain embodiments, this disclosure relates to methods of labeling intracellular nucleic acids by expressing in the cell RNA containing an aptamer sequence that specifically binds a label, e.g., fluorescent label, or placing in the cell nucleic acids containing an aptamer sequence that specifically binds a label, wherein the label comprises a carbene forming group, such as a diazirine group, under conditions such that the label specifically binds the aptamer sequence forming a complex; and exposing the complex to electromagnetic radiation sufficient for carbene formation resulting in a covalent bond to the aptamer sequence and the label.
- Salt Lake City UT, US Jennifer M. Heemstra - Salt Lake City UT, US
Assignee:
University of Utah Research Foundation - Salt Lake City UT
International Classification:
C12Q 1/6874 C12Q 1/6876 C12Q 1/6806 C12Q 1/6816
Abstract:
Branching phosphoramidite monomers and molecules having comb-like structures are disclosed and described. A branching phosphoramidite monomer having the structureis provided wherein R4 and R5 are independently —(O—CH-CH—)n where n is 1-5 or —O—(CH—)where n is 1-10, and R1, R2, and R3 are each one of dimethoxytrityl (DMT)—O—, levulinyl (Lev)—O—, and a phosphoramidite.
- Salt Lake City UT, US William M. Hanson - Salt Lake City UT, US Jennifer M. Heemstra - Salt Lake City UT, US
Assignee:
University of Utah Research Foundation - Salt Lake City UT
International Classification:
C12Q 1/68
Abstract:
Systems, devices, and methods for capturing single source-specific biological material from a multi-source aggregate of biological material are disclosed and discussed. A capture system is generated using reversible chain-blocking to make capture substrates having substrate-linked populations of capture molecules specific for molecules of interest. Incubating such capture substrates in the presence of only a single source of biological material facilitates the association of molecules of interest from the same source. Capture substrate-specific barcode sequences coupled to the capture molecules allow multisource aggregate processing and subsequent grouping to retain the source-specific information following downstream processing.
- Salt Lake City UT, US Jennifer M. Heemstra - Salt Lake City UT, US
Assignee:
University of Utah Research Foundation - Salt Lake City UT
International Classification:
G01N 33/94 C12N 15/115 G01N 33/53
Abstract:
Methods, assays, and products for the detection of small molecules are provided. In one aspect, for example, a method of detecting a small molecule in a sample can include reacting together a first half of a DNA split aptamer having a first reactive group coupled thereto, a second half of a DNA split aptamer having a second reactive group coupled thereto, where the DNA split aptamer is selective for the small molecule, and a sample containing the small molecule. The first half and the second half bind to the small molecule and the first reactive group and the second reactive group react to form an aptamer ligation product of the first half and the second half. The method can also include assaying for the aptamer ligation product in order to detect the small molecule presence in the sample.
- Salt Lake City UT, US Jennifer M. Heemstra - Salt Lake City UT, US
Assignee:
University of Utah Research Foundation - Salt Lake City UT
International Classification:
G01N 33/53
US Classification:
435 619, 536 231
Abstract:
Methods, assays, and products for the detection of small molecules are provided. In one aspect, for example, a method of detecting a small molecule in a sample can include reacting together a first half of a DNA split aptamer having a first reactive group coupled thereto, a second half of a DNA split aptamer having a second reactive group coupled thereto, where the DNA split aptamer is selective for the small molecule, and a sample containing the small molecule. The first half and the second half bind to the small molecule and the first reactive group and the second reactive group react to form an aptamer ligation product of the first half and the second half. The method can also include assaying for the aptamer ligation product in order to detect the small molecule presence in the sample.