Jennifer A Martinez

US Patent:

7914588, Mar 29, 2011

Filed:

Apr 10, 2007

Appl. No.:

11/786190

Inventors:

Jennifer S. Martinez - Santa Fe NM, US
R. Brian Dyer - Los Alamos NM, US
Dung M. Vu - Los Alamos NM, US
Yuping Bao - Tuscaloosa AL, US
Chang Zhong - Stanford CA, US

Assignee:

Los Alamos National Security, LLC - Los Alamos NM

International Classification:

C07F 1/10
C07F 1/12
A61K 33/00

US Classification:

977731, 977810, 556110, 424 934

Abstract:

Fluorescent metal nanoclusters were prepared.

US Patent:

8476014, Jul 2, 2013

Filed:

Feb 22, 2011

Appl. No.:

12/932321

Inventors:

Hsin-Chih Yeh - Los Alamos NM, US
James Henry Werner - Los Alamos NM, US
Jaswinder Kumar Sharma - Los Alamos NM, US
Jennifer Suzanne Martinez - Dixon NM, US

Assignee:

Los Alamos National Security, LLC - Los Alamos NM

International Classification:

C12Q 1/68
C12N 15/11

US Classification:

435 61, 536 231, 536 243

Abstract:

A hybridization probe containing two linear strands of DNA lights up upon hybridization to a target DNA using silver nanoclusters that have been templated onto one of the DNA strands. Hybridization induces proximity between the nanoclusters on one strand and an overhang on the other strand, which results in enhanced fluorescence emission from the nanoclusters.

US Patent:

8536119, Sep 17, 2013

Filed:

Mar 29, 2011

Appl. No.:

13/074246

Inventors:

Jennifer S. Martinez - Los Alamos NM, US
R. Brian Dyer - Los Alamos NM, US
Dung M. Vu - Los Alamos NM, US
Yuping Bao - Tuscaloosa AL, US
Chang Zhong - Stanford CA, US

Assignee:

Los Alamos National Security, LLC - Los Alamos NM

International Classification:

C07K 14/00

US Classification:

514 2, 977731

Abstract:

Fluorescent metal nanoclusters were prepared.

US Patent:

20060019244, Jan 26, 2006

Filed:

Jun 29, 2005

Appl. No.:

11/172246

Inventors:

Jennifer Martinez - Santa Fe NM, US
Basil Swanson - Los Alamos NM, US
Karen Grace - Los Alamos NM, US
Wyane Grace - Los Alamos NM, US
Andrew Shreve - Santa Fe NM, US

International Classification:

C12Q 1/70
C12Q 1/68
G01N 33/554
C12M 1/34
G01N 33/569

US Classification:

435005000, 435006000, 435007320, 435287200

Abstract:

An assay element is described including recognition ligands bound to a film on a single mode planar optical waveguide, the film from the group of a membrane, a polymerized bilayer membrane, and a self-assembled monolayer containing polyethylene glycol or polypropylene glycol groups therein and an assay process for detecting the presence of a biological target is described including injecting a biological target-containing sample into a sensor cell including the assay element, with the recognition ligands adapted for binding to selected biological targets, maintaining the sample within the sensor cell for time sufficient for binding to occur between selected biological targets within the sample and the recognition ligands, injecting a solution including a reporter ligand into the sensor cell; and, interrogating the sample within the sensor cell with excitation light from the waveguide, the excitation light provided by an evanescent field of the single mode penetrating into the biological target-containing sample to a distance of less than about 200 nanometers from the waveguide thereby exciting the fluorescent-label in any bound reporter ligand within a distance of less than about 200 nanometers from the waveguide and resulting in a detectable signal.

US Patent:

20060019321, Jan 26, 2006

Filed:

Jun 29, 2005

Appl. No.:

11/172244

Inventors:

Jennifer Martinez - Santa Fe NM, US
Basil Swanson - Los Alamos NM, US
John Shively - Arcadia CA, US
Lin Li - Monrovia CA, US

International Classification:

G01N 33/574
C12M 1/34

US Classification:

435007230, 435287200

Abstract:

An assay element is described including recognition ligands adapted for binding to carcinoembryonic antigen (CEA) bound to a film on a single mode planar optical waveguide, the film from the group of a membrane, a polymerized bilayer membrane, and a self-assembled monolayer containing polyethylene glycol or polypropylene glycol groups therein and an assay process for detecting the presence of CEA is described including injecting a possible CEA-containing sample into a sensor cell including the assay element, maintaining the sample within the sensor cell for time sufficient for binding to occur between CEA present within the sample and the recognition ligands, injecting a solution including a reporter ligand into the sensor cell; and, interrogating the sample within the sensor cell with excitation light from the waveguide, the excitation light provided by an evanescent field of the single mode penetrating into the biological target-containing sample to a distance of less than about 200 nanometers from the waveguide thereby exciting any bound reporter ligand within a distance of less than about 200 nanometers from the waveguide and resulting in a detectable signal.

US Patent:

20140349289, Nov 27, 2014

Filed:

May 22, 2014

Appl. No.:

14/284776

Inventors:

- Los Alamos NM, US
James Werner - Los Alamos NM, US
Jennifer S. Martinez - Dixon NM, US

International Classification:

C12Q 1/68

US Classification:

435 611, 536 2431

Abstract:

Described herein are nucleic acid based probes and methods for discriminating and detecting single nucleotide variants in nucleic acid molecules (e.g., DNA). The methods include use of a pair of probes can be used to detect and identify polymorphisms, for example single nucleotide polymorphism in DNA. The pair of probes emit a different fluorescent wavelength of light depending on the association and alignment of the probes when hybridized to a target nucleic acid molecule. Each pair of probes is capable of discriminating at least two different nucleic acid molecules that differ by at least a single nucleotide difference. The methods can probes can be used, for example, for detection of DNA polymorphisms that are indicative of a particular disease or condition.