Jennifer Erin Padilla

US Patent:

20060234261, Oct 19, 2006

Filed:

Mar 7, 2006

Appl. No.:

11/371347

Inventors:

Niles Pierce - Pasadena CA, US
Robert Dirks - Pasadena CA, US
Jennifer Padilla - Monrovia CA, US

International Classification:

C12Q 1/68

US Classification:

435006000, 977924000

Abstract:

The present invention relates to the use of calorimetric hybridization chain reaction (HCR) to detect the presence of one or more target analytes in a sample. In the preferred embodiments, metastable nucleic acid monomers are provided that associate in the presence of an initiator nucleic acid. Upon exposure to the initiator, the monomers self-assemble in a hybridization chain reaction. The monomers themselves may be conjugated to nano-gold particles. In other embodiments, a detection component is provided that comprises nano-gold particles and is able to bind to or associate with polymerized monomers. Thus, self-assembly of the HCR monomers leads to aggregation of nano-gold particles and a detectable change in sample color.

US Patent:

20070218547, Sep 20, 2007

Filed:

Mar 31, 2004

Appl. No.:

10/815543

Inventors:

Todd Yeates - Agoura Hills CA, US
Jennifer Padilla - Pasadena CA, US
Stephanie Yoshida - Seattle WA, US
Chris Colovos - Thousand Oaks CA, US

International Classification:

C12N 5/06
C07H 21/04
C07K 14/00
C12N 15/00

US Classification:

435325000, 435320100, 530350000, 536023400

Abstract:

Self-assembling fusion proteins and nucleic acids encoding the same are provided. The subject fusion proteins include a first dimer forming oligomerization domain and a second tetramer forming oligomerization domain rigidly linked to each other. Also provided are regular structures made up of a plurality of self-assembled fusion proteins of the subject invention, and methods for producing the same. The subject fusion proteins find use in the preparation of self-assembled nanostructures, e.g., two-dimensional layers and three-dimensional networks, which structures find use in a variety of different applications.