Shea N. Gardner - San Leandro CA, US Allen T. Christian - Tracy CA, US Jennifer A. Young - Berkeley CA, US David S. Clague - Livermore CA, US
Assignee:
Lawrence Livermore National Security, LLC - Livermore CA
International Classification:
C12P 19/34 C12Q 1/68
US Classification:
435 912, 435 6
Abstract:
A method of fabricating a DNA molecule of user-defined sequence. The method comprises the steps of preselecting a multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence, separating the DNA sequence segments temporally, and combining the multiplicity of DNA sequence segments with at least one polymerase enzyme wherein the multiplicity of DNA sequence segments join to produce the DNA molecule of user-defined sequence. Sequence segments may be of length n, where n is an even or odd integer. In one embodiment the length of desired hybridizing overlap is specified by the user and the sequences and the protocol for combining them are guided by computational (bioinformatics) predictions. In one embodiment sequence segments are combined from multiple reading frames to span the same region of a sequence, so that multiple desired hybridizations may occur with different overlap lengths. In one embodiment starting sequence fragments are of different lengths, n, n+1, n+2, etc.
Sequential Addition Of Short Dna Oligos In Dna-Polymerase-Based Synthesis Reactions
Shea N. Gardner - San Leandro CA, US Allen T. Christian - Tracy CA, US Jennifer A. Young - Berkeley CA, US David S. Clague - Livermore CA, US
Assignee:
Lawrence Livermore National Security, LLC - Livermore CA
International Classification:
C12Q 1/68 C12P 19/34
US Classification:
435 612, 435 912
Abstract:
A method of preselecting a multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence, separating the DNA sequence segments temporally, and combining the multiplicity of DNA sequence segments with at least one polymerase enzyme wherein the multiplicity of DNA sequence segments join to produce the DNA molecule of user-defined sequence. Sequence segments may be of length n, where n is an odd integer. In one embodiment the length of desired hybridizing overlap is specified by the user and the sequences and the protocol for combining them are guided by computational (bioinformatics) predictions. In one embodiment sequence segments are combined from multiple reading frames to span the same region of a sequence, so that multiple desired hybridizations may occur with different overlap lengths.
Apr 2010 to 2000 VOLUNTEERThe Leukemia & Lymphoma Society San Francisco, CA Nov 2013 to Nov 2014 Logistics Specialist, Team In TrainingThe Leukemia & Lymphoma Society San Francisco, CA Jul 2013 to Oct 2013 Campaign Assistant, Light The NightBOUDIN BAKERY San Francisco, CA Jun 2009 to May 2013 Human Resources GeneralistAlphonse Berber Gallery Berkeley, CA Feb 2009 to May 2009 Gallery AssistantROBERT KOCH GALLERY San Francisco, CA Jun 2008 to Aug 2008 InternROBERT KOCH GALLERY
Jun 2008 to Aug 2008 Editor Intern
Education:
UNIVERSITY OF CALIFORNIA, BERKELEY Berkeley, CA May 2009 B.A. in History of Art
Skills:
Research, Word, Excel, Proofreading, Grammar, Editing, Report writing, data entry, event logistics, event planning, non-profit management
Jun 2011 to Present Digital Strategy CoordinatorMfg Productions Los Angeles, CA Aug 2010 to Jun 2011 Marketing Coordinator4Medica Medical Group Los Angeles, CA Jun 2009 to Aug 2010 Marketing AssistantVitamin Water San Francisco, CA Jun 2007 to Dec 2008 Field Marketing ManagerRed Bull North America San Francisco, CA Mar 2004 to Jun 2007 Brand Ambassador
Education:
Mills College Oakland, CA May 2008 Masters of Business Administration in Marketing Management SpecializationMills College Oakland, CA May 2007 B.A. in Business-Economics
Dr. Adrian Vogt, DDS San Mateo, CA Jun 2004 to Oct 2012 Office ManagerDr. Alan Goodwin DDS San Mateo, CA May 1999 to Aug 2003 Financial CoordinatorDr. William Green, DDS Ukiah, CA Oct 1997 to May 1999 Financial Coordinator/Orthodontic Assistant
Medicine Doctors
Dr. Jennifer Young, San Francisco CA - MD (Doctor of Medicine)
Dr. Young graduated from the University of Pennsylvania School of Medicine in 2005. She works in Leesburg, VA and 2 other locations and specializes in Pediatrics. Dr. Young is affiliated with Adventist Healthcare Shady Grove Medical Center, Inova Loudoun Hospital and Reston Hospital Center.
Center For Pain Supportive Care 4611 E Shea Blvd STE 190, Phoenix, AZ 85028 4808890180 (phone), 4808890186 (fax)
Languages:
English Spanish
Description:
Ms. Young works in Phoenix, AZ and specializes in Anesthesiology. Ms. Young is affiliated with Banner University Medical Center Phoenix, HonorHealth Scottsdale Osborn Medical Center and HonorHealth Scottsdale Shea Medical Center.
Gig Harbor, WA Tacoma, WA South Bend, IN San Leandro, CA
Work:
Home with sweet babies - Mommy (2003) Doug Fox Travel -Puyallup, WA - Travel agent (1997-1998) Anthony Travel - Notre Dame, IN - Travel agent (1998-1999) Travel Service Montclair - Oakland, CA - Travel agent (1999-2000) Simpson Investments - Tacoma, WA - Executive assistant Doug fox travel Anthony travel
Education:
Up with people
Relationship:
Married
Tagline:
I am a happy, homeschooling, patriotic, DIY-ing, healthy-living, Christian, family lady.
Bragging Rights:
Wife of 1 Superman, mother of 5 Wonderkids
Jennifer Young
Work:
Barnsley Gardens Resort - Server Assistant (2013) Bella Roma - Server (2013) Wings of Knowledge - Tutor (2012-2013) Froyolo - Cashier (2012-2012) Red Lobster - Trainee Server (2012-2012) Positively Pools - Lifeguard (2010-2010) Camp Rainey Mountain - Instructor (2008-2009) The Dublin - Server (2011-2011)
Education:
Georgia Institute of Technology - Environmental Engineering, Gwinnett School of Math, Science, and Tech, Brookwood High School, North Georgia College and State University - Physics
Jennifer Young
Work:
JJYoung Inc. - Stay at home Mom (2012) Coahoma ISD - Teacher/coach (2004-2005) Argyle ISD - Teacher/coach (2005-2007) Snyder ISD - Teacher/coach (2007-2012)
Education:
McMurry University - Education/Business, Midland College - Business, Hermleigh ISD - HS Diploma
About:
Saved by the grace of God, I am a daughter of The King. Married Jeremiah, March 2009, parents to a beautiful daughter, Emmalyn as of July 2010. Jeremiah works as the assistant county auditor and I am ...
Tagline:
Christian woman who loves the lord,my husband, daughter and family. God is so good!
Bragging Rights:
Haiti Mission Trip
Jennifer Young
Lived:
Oakland, CA Berkeley, CA Irvine, CA
Work:
Boudin Bakery - Office Coordinator (2009)
Education:
University Of California Berkeley - Art History
Relationship:
In_a_relationship
Jennifer Young
Work:
Three Geeks and a Needle - Geek 3 (2011) Northwestern University - Librarian (2003) Saint Louis University - Librarian (1998-2003)
Education:
Bradley University - History/Secondary Education, University of Illinois at Urbana-Champaign - Library & Information Science
Relationship:
In_a_relationship
Jennifer Young
Work:
State Farm Insurance - Systems Analyst (2010)
Education:
Illinois State University - Information Systems
About:
From Peoria, working for SF
Tagline:
Everyday wife
Bragging Rights:
Completed college, bought first house at age 21
Jennifer Young
Lived:
San Francisco, CA
Work:
Insperity - District Manager (2008)
Education:
Villanova
Jennifer Young
Work:
University of California San Francisco - Research Associate (2009-2011)
Education:
Stanford University School of Medicine, Massachusetts Institute of Technology - Biology, Phillips Exeter Academy
Youtube
Jennifer Young
Category:
Music
Uploaded:
28 Oct, 2009
Duration:
3m 35s
jennifer young
jennifer young
Category:
People & Blogs
Uploaded:
05 Nov, 2008
Duration:
27s
Travis Larson & Jennifer Young - Good and The...
Travis Larson & Jennifer Young perform Good and The Bad from the Travi...
Category:
Music
Uploaded:
05 Sep, 2010
Duration:
3m 16s
Sam Botta & Jennifer Young Night of 100 Stars
Gig Young, Oscar Winner for "They Shoot Horses Don't They" was Jennife...
Category:
News & Politics
Uploaded:
29 Apr, 2009
Duration:
1m 54s
Jennifer Young - AM Philadelphia, Harcopy, & ET
Jennifer Young's appearances on AM Philadelphia, Harcopy, and Entertai...
Category:
Entertainment
Uploaded:
10 Oct, 2008
Duration:
5m 8s
Jennifer Young - The Insider
www.myspace.com/... Jennifer Young's appearance on the television new...