Jerome L Bailey

US Patent:

58077480, Sep 15, 1998

Filed:

Apr 11, 1997

Appl. No.:

8/765398

Inventors:

Jerome Bailey - Sunnyvale CA

Assignee:

City of Hope - Duarte CA

International Classification:

G01N 3368
C07D23504

US Classification:

436 89

Abstract:

Novel alkoxythiocarbonylimidazoles provide new reagents for the N-terminal sequencing of small polypeptide samples. These reagents form an alkoxy thiourea derivative which is cleaved with acid to remove the N-terminal amino acid as a stable thiazolinone which does not rearrange to a thiohydantoin. This thiazolinone may be derivatized to provide a detectable group such as a fluorescent group or ionizable group detectable by mass spectrometry.

US Patent:

59982136, Dec 7, 1999

Filed:

Nov 9, 1995

Appl. No.:

8/532667

Inventors:

Jerome M. Bailey - Sunnyvale CA
John E. Shively - Arcadia CA

Assignee:

City of Hope - Duarte CA

International Classification:

G01N 3368

US Classification:

436 89

Abstract:

A carboxy terminal protein sequencing process is disclosed which utilizes diphenyl phosphoroisothiocyanatidate and a heterocyclic amine to produce a thiohydantoin derivative of the C-terminal amino acid. The derivative is readily cleaved. The method is useful to sequence through all of the 20 naturally occuring amino acids.