Jessica Anderson

US Patent:

20090263843, Oct 22, 2009

Filed:

Mar 26, 2009

Appl. No.:

12/383832

Inventors:

Jessica Anderson - Monticello WI, US
Poncho Meisenheimer - San Luis Obispo CA, US
John Shultz - Verona WI, US

Assignee:

Promega Corporation - Madison WI

International Classification:

C12Q 1/34
C12N 9/00
C12Q 1/02
G01N 33/68
C07D 417/14
C07K 1/13
C07D 493/10
C07D 495/04

US Classification:

435 18, 435 4, 435 29, 436 86, 530344, 530345, 548147, 548159

Abstract:

The invention provides compounds and methods for site-specifically labeling proteins with cyanobenzothiazole derivatives of formula I. For example, the invention provides methods for labeling the N-terminus of a protein that terminates with a cysteine residue. The invention also provides methods for isolating an N-terminally labeled protein and methods for detecting an N-terminally labeled protein.

US Patent:

20100075351, Mar 25, 2010

Filed:

Sep 9, 2009

Appl. No.:

12/556505

Inventors:

Jessica Anderson - Monticello WI, US
Poncho Meisenheimer - San Luis Obispo CA, US
John Shultz - Verona WI, US
James J. Cali - Verona WI, US
Dongping Ma - Madison WI, US

Assignee:

Promega Corporation - Madison WI

International Classification:

C12Q 1/66
C07K 5/10

US Classification:

435 8, 530330

Abstract:

The invention provides methods that employ derivatives of 2-cyano-6-hydroxy- or 2-cyano-6-amino-benzothiazole, for example, in a bioluminogenic reaction. Also provided are novel compounds that can be used in the methods. The invention further provides methods for detecting or determining the presence of molecules and/or enzymes, the modulator activity of such molecules, and/or the activity of such enzymes. The methods are adaptable to high-throughput format.