Skills:
[Molecular Biology]: Mammalian cell culture (primary cell and cell lines), RNA/DNA isolation, RNA quantity and quality assessment, RNA Gel electroporation, Primer design and qualification for real time RT-PCR, Real time RT-PCR. Genotyping, Site directed mutagenesis, Gene transfection, Virus infection, Dual-reporter gene assay, Mammalian two-hybrid assay, Plasmid mini/maxi-prep, Western blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescent, Immunocytochemistry, ELISA/EIA, BCA protein assay, SDS-PAGE, Chromatin association assay, Nuclear protein extraction, Membrane fractionation, MTT cell growth assay, Thin-layer chromatography, Plasmid maxi/mini prep, Plasmid enzyme digestion. [Protein Technique]: Protein/peptide fractionation by preparative High Performance Liquid Chromatography (HPLC), Peptide analysis by analytical HPLC, Ion exchange chromatography, Degree of hydrolysis by OPA, Soluble Solid Index (SSI). [Animal Handling]: Mouse perfusion, Embryonic and postnatal tissue harvesting, Animal husbandry. [Major Instruments]: preparative and analytical HPLC (Agilent 1200), Real time RT-PCR machine (Stratagene and Biorad), UV spectrophotometer, Electroporater, Turner TD-20/20 luminometer, Beckman LS 6500 Liquid Scintillation Counter