Jian Jim Hua

US Patent:

8623379, Jan 7, 2014

Filed:

Jan 3, 2003

Appl. No.:

10/336566

Inventors:

Harriet L. Robinson - Atlanta GA, US
James Smith - Decatur GA, US
Jian Hua - Dunwoody GA, US

Assignee:

Emory University - Atlanta GA

International Classification:

C12Q 1/70
C12N 15/63
C12N 15/33
C12N 15/48
C12N 15/49
A61K 39/21

US Classification:

4241991, 4242041, 4242081, 4242321, 4353201

Abstract:

We have developed DNA and viral vectors that can be used, alone or in combination, as a vaccine against one HIV clade, subtype, or recombinant form of HIV or against multiple HIV clades, subtypes, or recombinant forms. Moreover, the vectors can encode a variety of antigens, which may be obtained from one clade or from two or more different clades, and the antigens selected and/or the manner in which the vectors are formulated (e. g. , mixed) can be manipulated to generate a protective immune response against a variety of clades (e. g. , the clades to which a patient is most likely to be exposed; with the proportions of the components of the vaccine tailored to the extent of the patient's risk to a particular clade or clades).

US Patent:

20020106798, Aug 8, 2002

Filed:

Mar 2, 2001

Appl. No.:

09/798675

Inventors:

Harriet Robinson - Atlanta GA, US
James Smith - Tucker GA, US
Ted Ross - Winterville NC, US
Rick Bright - Atlanta GA, US
Jian Hua - Decatur GA, US
Dennis Ellenberger - Norcross GA, US
Donald Hildebrand - Athens GA, US

International Classification:

C12N015/86
C12N007/00

US Classification:

435/456000, 435/320100, 435/235100

Abstract:

The present invention relates to novel plasmid constructs useful for the delivery of DNA vaccines. The present invention provides novel plasmids having a transcription cassette capable of directing the expression of a vaccine nucleic acid insert encoding immunogens derived from any pathogen, including fungi, bacteria and viruses. The present invention, however, is particularly useful for inducing in a patient an immune response against pathogenic viruses such as HIV, measles or influenza. Immunodeficiency virus vaccine inserts of the present invention express non-infectious HIV virus-like particles (VLP) bearing multiple viral epitopes. VLPs allow presentation of the epitopes to multiple histocompatability types, thereby reducing the possibility of the targeted virus escaping the immune response. Also described are methods for immunizing a patient by delivery of a novel plasmid of the present invention to the patient for expression of the vaccine insert therein. Optionally, the immunization protocol may include a booster vaccination that may be a live vector vaccine such as a recombinant pox virus or modified vaccinia Arbora vector. The booster live vaccine vector includes a transcription cassette expressing the same vaccine insert as the primary immunizing vector.

US Patent:

20040105871, Jun 3, 2004

Filed:

Mar 8, 2002

Appl. No.:

10/093953

Inventors:

Harriet Robinson - Atlanta GA, US
James Smith - Decatur GA, US
Jian Hua - Dunwoody GA, US

International Classification:

A61K039/12
C12N007/00
C12N015/863

US Classification:

424/199100, 435/235100, 435/456000

Abstract:

The present invention relates to novel plasmid constructs useful for the delivery of DNA vaccines. The present invention provides novel plasmids having a transcription cassette capable of directing the expression of a vaccine nucleic acid insert encoding immunogens derived from any pathogen, including fungi, bacteria and viruses. The present invention, however, is particularly useful for inducing in a patient an immune response against pathogenic viruses such as HIV, measles or influenza. Immunodeficiency virus vaccine inserts of the present invention express non-infectious HIV virus-like particles (VLP) bearing multiple viral epitopes. VLPs allow presentation of the epitopes to multiple histocompatability types, thereby reducing the possibility of the targeted virus escaping the immune response. Also described are methods for immunizing a patient by delivery of a novel plasmid of the present invention to the patient for expression of the vaccine insert therein. Optionally, the immunization protocol may include a booster vaccination that may be a live vector vaccine such as a recombinant pox virus or modified vaccinia Arbora vector. The booster live vaccine vector includes a transcription cassette expressing the same vaccine insert as the primary immunizing vector.

US Patent:

20070048861, Mar 1, 2007

Filed:

Jan 17, 2006

Appl. No.:

11/333770

Inventors:

Harriet Robinson - Atlanta GA, US
James Smith - Cumming GA, US
Jian Hua - Dunwoody GA, US
Bernard Moss - Bethesda MA, US
Rama Amara - Atlanta GA, US
Linda Wyatt - Rockville MD, US
Patricia Earl - Chevy Chase MA, US
Ted Ross - Aspinall PA, US
Rick Bright - Washington DC, US
Salvatore Butera - Atlanta GA, US
Dennis Ellenberger - Norcross GA, US
Thomas Folks - Snellville GA, US

International Classification:

C12P 21/06
C12N 15/00

US Classification:

435320100, 435069100

Abstract:

The present invention relates to novel plasmid constructs useful for the delivery of DNA vaccines. The present invention provides novel plasmids having a transcription cassette capable of directing the expression of a vaccine nucleic acid insert encoding immunogens derived from any pathogen, including fungi, bacteria and viruses. The present invention, however, is particularly useful for inducing in a patient an immune response against pathogenic viruses such as HIV, measles or influenza. Immunodeficiency virus vaccine inserts of the present invention express non-infectious HIV virus-like particles (VLP) bearing multiple viral epitopes. VLPs allow presentation of the epitopes to multiple histocompatability types, thereby reducing the possibility of the targeted virus escaping the immune response. Also described are methods for immunizing a patient by delivery of a novel plasmid of the present invention to the patient for expression of the vaccine insert therein. Optionally, the immunization protocol may include a booster vaccination that may be a live vector vaccine such as a recombinant pox virus or modified vaccinia Arbora vector. The booster live vaccine vector includes a transcription cassette expressing the same vaccine insert as the primary immunizing vector.

US Patent:

20150231227, Aug 20, 2015

Filed:

Mar 29, 2010

Appl. No.:

12/749164

Inventors:

Harriet L. Robinson - Atlanta GA, US
James M. Smith - Cumming GA, US
Jian Hua - Dunwoody GA, US
Bernard Moss - Bethesda MA, US
Rama R. Amara - Atlanta GA, US
Linda S. Wyatt - Rockville MD, US
Patricia L. Earl - Chevy Chase MA, US
Ted M. Ross - Aspinall PA, US
Rick A. Bright - Washington DC, US
Salvatore T. Butera - Atlanta GA, US
Dennis L. Ellenberger - Norcross GA, US
Thomas M. Folks - Snellville GA, US

International Classification:

A61K 39/21
A61K 39/145
C12N 7/00
A61K 39/165

Abstract:

Novel plasmid constructs useful for the delivery of DNA vaccines are provided having a transcription cassette capable of directing the expression of a vaccine nucleic acid insert encoding immunogens derived from any pathogen, including fungi, bacteria and viruses. The constructs are useful for inducing in a patient an immune response against pathogenic viruses such as HIV, measles or influenza. Immunodeficiency virus vaccine inserts of the present invention express non-infectious HIV virus-like particles (VLP) bearing multiple viral epitopes. VLPs allow presentation of the epitopes to multiple histocompatability types. Also described are methods for immunizing a patient by delivery of a novel plasmid of the present invention to a patient. Optionally, the immunization protocol may include a booster vaccination that may be a live vector vaccine such as a recombinant pox virus or modified vaccinia Arbora vector having a transcription cassette expressing the same vaccine insert as the primary immunizing vector.