Jin Yao Zhang

US Patent:

7982021, Jul 19, 2011

Filed:

Jul 16, 2007

Appl. No.:

11/826519

Inventors:

Jin Zhang - Baltimore MD, US
Bharath Ananthanarayanan - Scotch Plains NJ, US

Assignee:

The Johns Hopkins University - Baltimore MD

International Classification:

C07H 21/02
C08H 1/00

US Classification:

536 231, 435 697

Abstract:

Nucleic acid molecules encoding emission ratiometric indicators of phosphoinositides, which comprise a fusion protein comprising a pleckstrin homology (PH) domain of Akt (also known as protein kinase B) and a “pseudoligand” containing acidic amino acid residues, which is sandwiched between resonance energy transfer (RET) pairs, such as cyan and yellow mutants of GFP (a FRET pair). The nucleic acid molecules can be used, for example, to produce the indicators which can be used, inter alia, to monitor spatiotemporal dynamics of phosphoinositides and in high throughput assays for inhibitors of PI3K, including drug screening assays.

US Patent:

8080235, Dec 20, 2011

Filed:

Oct 26, 2006

Appl. No.:

12/091858

Inventors:

Jin Zhang - Baltimore MD, US
Qiang Ni - Baltimore MD, US
Michael David Allen - Baltimore MD, US

Assignee:

The Johns Hopkins University - Baltimore MD

International Classification:

G01N 33/52
G01N 33/533
G01N 33/542
A61K 38/00

US Classification:

424 96, 424 52, 536 266, 436546, 436800

Abstract:

Protein kinase A reporters useful for obtaining measurements of protein kinase A activity with high spatial and temporal resolution can be used in high throughput assays to identify potentially therapeutic compounds.

US Patent:

8236523, Aug 7, 2012

Filed:

Oct 26, 2006

Appl. No.:

11/586707

Inventors:

Jin Zhang - Baltimore MD, US
Lisa Marie DiPilato - Baltimore MD, US

Assignee:

The Johns Hopkins University - Baltimore MD

International Classification:

C12P 21/06
C12N 1/00

US Classification:

435 691, 435243, 435 697, 435 698

Abstract:

cAMP reporters useful for obtaining measurements of cAMP levels with high spatial and temporal resolution and in high throughput assays.

US Patent:

8615118, Dec 24, 2013

Filed:

May 31, 2011

Appl. No.:

13/149171

Inventors:

Byong Yong Yi - Ellicott City MD, US
Xinsheng Cedric Yu - Clarksville MD, US
Jin Zhang - Catonsville MD, US
Giovanni Lasio - Towson MD, US

Assignee:

The University of Maryland, Baltimore - Baltimore MD

International Classification:

G06K 9/00

US Classification:

382128, 382131, 382132

Abstract:

Techniques for background subtraction in computed tomography include determining voxels in a slice of interest in a three dimensional computed tomography scan of the interior of a body based on a first set of measurements of radiation transmitted through the body. Based on the first set of measurements, a first background image for radiation transmitted through the body in a first direction is determined without the effects of the voxels in the slice of interest. A current image is determined based on a different current measurement of radiation transmitted through the body in the first direction. A first difference is determined between the current image and the first background image. The result is a high contrast image in the slice of interest even from a single current projection image.

US Patent:

20050026234, Feb 3, 2005

Filed:

May 28, 2004

Appl. No.:

10/857622

Inventors:

Jonathan Violin - Durham NC, US
Alexandra Newton - San Diego CA, US
Roger Tsien - La Jolla CA, US
Jin Zhang - Baltimore MD, US

International Classification:

C12Q001/68
C12Q001/42
C07K014/47

US Classification:

435021000, 530352000

Abstract:

A chimeric phosphorylation indicator (CPI) as provided herein can contain a donor molecule, a phosphorylatable domain, a phosphoaminoacid binding domain (PAABD), and an acceptor molecule. Where the phosphorylatable domain is phosphorylatable by protein kinase C (PKC), the CPI is a c-kinase activity reporter (CKAR). Donor and acceptor molecules may be, independently, fluorescent proteins such as non-oligomerizing fluorescent proteins. A CPI can contain a phosphorylatable polypeptide and a fluorescent protein; the phosphorylatable polypeptide may be contained within the sequence of the fluorescent protein, or the fluorescent protein may be contained within the sequence of the phosphorylatable polypeptide. The spatiotemporal properties of the PKC signal pathway may be tested with CKAR, calcium-sensing fluorophores and FRET-based translocation assays. Polynucleotides encoding such CPIs, and kits containing the indicators and/or the polynucleotides, are provided. A method of using the chimeric phosphorylation indicators to detect a kinase or phosphatase in a sample is provided.

US Patent:

20100202970, Aug 12, 2010

Filed:

Aug 20, 2008

Appl. No.:

12/674008

Inventors:

Jin Zhang - Baltimore MD, US
Robert Howard Newman - Baltimore MD, US
Xinxin Gao - Baltimore MD, US
Michael David Allen - Baltimore MD, US
Matthew David Fosbrink - Baltimore MD, US

Assignee:

The Johns Hopkins University - Baltimore MD

International Classification:

A61K 49/00
C07H 21/00
C12Q 1/00
G01N 33/53
C07K 14/00
A61P 43/00

US Classification:

424 91, 536 231, 435 4, 435 72, 530300

Abstract:

The invention provides a variety of molecular tools for use in live-cell tracking of activities of biomolecules and in high-throughput drug screening.

US Patent:

20110165602, Jul 7, 2011

Filed:

Feb 23, 2011

Appl. No.:

13/032872

Inventors:

Jin Zhang - Rosedale MD, US
Bharath Ananthanarayanan - Scotch Plains NJ, US

Assignee:

JOHNS HOPKINS UNIVERSITY - Baltimore MD

International Classification:

C12Q 1/48
C12N 9/12
C07H 21/00
C12N 5/10

US Classification:

435 15, 435194, 536 232, 435325

Abstract:

Emission ratiometric indicators of phosphoinositides comprise a fusion protein comprising a pleckstrin homology (PH) domain of Akt (also known as protein kinase B) and a “pseudoligand” containing acidic amino acid residues, which is sandwiched between resonance energy transfer (RET) pairs, such as cyan and yellow mutants of GFP (a FRET pair). Such indicators can be used, inter alia, to monitor spatiotemporal dynamics of phosphoinositides and in high throughput assays for inhibitors of PI3K, including drug screening assays.

US Patent:

20110275103, Nov 10, 2011

Filed:

Apr 28, 2011

Appl. No.:

13/096689

Inventors:

Jin Zhang - Rosedale MD, US
Xinxin Gao - Baltimore MD, US

Assignee:

THE JOHNS HOPKINS UNIVERSITY - Baltimore MD

International Classification:

C12Q 1/48
C12N 15/62
B65D 85/84
C12N 1/21
C12N 1/19
C12N 5/10
C12N 11/02
C12N 15/63

US Classification:

435 15, 435177, 536 232, 4353201, 4352523, 4352542, 435419, 435325, 435366, 2065246

Abstract:

Phosphoinositide-dependent kinase 1 (PDK1) activity reporters can be used in high throughput assays for drug screening.