Jing Li

US Patent:

6410330, Jun 25, 2002

Filed:

Jul 27, 2001

Appl. No.:

09/917533

Inventors:

Yi Li - Miami FL
Jing Li - Miami FL
Ted W. Britton - Sunrise FL

Assignee:

Coulter International Corp. - Miami FL

International Classification:

G01N 3100

US Classification:

436 10, 436 8, 436 17, 436 63, 436149, 436150, 436164, 422 73, 422 8201, 422 8202, 422 8209, 435 2, 435 29, 435 34, 435 39

Abstract:

A method for differentiating and enumerating nucleated red blood cells in a blood sample is described. The method includes the steps of lysing red blood cells of a blood sample with a lytic reagent, measuring nucleated blood cells by DC impedance measurement in a non-focused flow aperture, differentiating nucleated red blood cells from other cell types, and reporting nucleated red blood cells in the blood sample. The method further includes subtracting nucleated red blood cells and other interference materials from the count of remaining blood cells, and reporting a corrected white blood cell count of the blood sample. Additionally, the method further includes measuring spectrophotometric absorbance of the sample mixture at a predetermined wavelength of a hemoglobin chromogen formed upon lysing the blood sample, and reporting hemoglobin concentration of the blood sample.

US Patent:

6573102, Jun 3, 2003

Filed:

Jul 27, 2001

Appl. No.:

09/917530

Inventors:

Yi Li - Miami FL
Jing Li - Miami FL

Assignee:

Coulter International Corp. - Miami FL

International Classification:

G01N 3100

US Classification:

436 17, 436 8, 436 10, 436 18, 436 63, 2524081, 435 2, 435 29

Abstract:

A lytic reagent composition for measuring nucleated blood cells in a blood sample is described. The lytic reagent composition contains a quaternary ammonium surfactant, an ethoxylated phenol, and an ethoxylated alcohol. When mixed with a blood sample, the lytic reagent composition lyses red blood cells and enables a differentiation of nucleated red blood cells from other cell types by DC impedance measurement. The lytic reagent composition can further contain an organic ligand for determining total hemoglobin concentration of a blood sample photometrically. Further disclosed is a lytic reagent system including the lytic reagent composition and a diluent. In addition, a single reagent composition containing salts is also disclosed, which can be used without a separate diluent. The lytic reagent compositions can be used for concurrent measurement of nucleated red blood cells, WBC, and hemoglobin of a blood sample.

US Patent:

6673618, Jan 6, 2004

Filed:

Aug 23, 2002

Appl. No.:

10/226800

Inventors:

Yi Li - Miami FL
Jing Li - Miami FL
Ted W. Britton - Sunrise FL

Assignee:

Coulter International Corp. - Miami FL

International Classification:

G01N 3100

US Classification:

436 10, 436 8, 436 17, 436 63, 436149, 436150, 436164, 422 73, 422 8201, 422 8202, 422 8209, 435 2, 435 29, 435 34, 435 39

Abstract:

A method for differentiating and enumerating nucleated red blood cells in a blood sample is described. The method includes the steps of lysing red blood cells of a blood sample with a lytic reagent, measuring nucleated blood cells by DC impedance measurement in a non-focused flow aperture, differentiating nucleated red blood cells from other cell types, and reporting nucleated red blood cells in the blood sample. The method further includes subtracting nucleated red blood cells and other interference materials from the count of remaining blood cells, and reporting a corrected white blood cell count of the blood sample. Additionally, the method further includes measuring spectrophotometric absorbance of the sample mixture at a predetermined wavelength of a hemoglobin chromogen formed upon lysing the blood sample, and reporting hemoglobin concentration of the blood sample.

US Patent:

6916658, Jul 12, 2005

Filed:

Feb 2, 2004

Appl. No.:

10/770193

Inventors:

Jing Li - Miami FL, US
Yi Li - Miami FL, US

Assignee:

Beckman Coulter, Inc. - Fullerton CA

International Classification:

G01N031/00
G01N033/48

US Classification:

436 10, 436 8, 436 17, 436 63, 436149, 436150, 422 73, 422 8201, 422 8202, 435 2, 435 29, 435 34, 435 39

Abstract:

A method for measuring immature granulocytes in a blood sample is described. The method includes the steps of lysing red blood cells of a blood sample with a lytic reagent, analyzing the sample mixture by DC impedance measurement, determining immature granulocytes from obtained DC histogram, and reporting immature granulocytes in the blood sample. The method further includes measuring nucleated red blood cells in the blood sample.

US Patent:

7198953, Apr 3, 2007

Filed:

Oct 7, 2004

Appl. No.:

10/960370

Inventors:

Nery Ortiz - Miami FL, US
Jing Li - Miami FL, US
Yi Li - Miami FL, US
Sandra Socarras - Miami FL, US

Assignee:

Beckman Coulter, Inc. - Fullerton CA

International Classification:

G01N 33/48
G01N 31/00

US Classification:

436 63, 436 10, 436164, 436166

Abstract:

Methods of using a reference control composition containing a nucleated red blood cell component for measurement of nucleated red blood cells are disclosed. The nucleated red blood cell component is made of stabilized or processed nucleated blood cells which have impedance and optical properties simulating the impedance and optical properties of human nucleated red blood cells under a blood lysing condition as measured by a specific measurement. The methods include mixing the reference control composition with a lytic reagent, and analyzing the control sample mixture on a flow cytometric instrument by impedance, impedance and optical measurement, or DC impedance and a multi-dimensional measurement of light scatter, radio frequency and a second DC impedance; and reporting the simulated nucleated red blood cells in the reference control composition.

US Patent:

7482165, Jan 27, 2009

Filed:

Aug 24, 2005

Appl. No.:

11/210346

Inventors:

Min Zheng - Pembroke Pines FL, US
Jing Li - Miami FL, US
Yi Li - Miami FL, US
Michael J. Switzer - Coral Springs FL, US

Assignee:

Beckman Coulter, Inc. - Fullerton CA

International Classification:

G01N 33/48

US Classification:

436 63, 436 8, 436 10, 436 17, 436 70, 436174, 436175, 435 2, 422 73

Abstract:

Methods of prevention and correction of white blood cell interferences to the measurements of mean cell volume (MCV), red blood cell concentration (RBC) and hematocrit (Hct) of a blood sample are disclosed. The interference to MCV can be prevented by identifying a valley between the red blood cell and white blood cell modes on the red blood cell distribution histogram, defining a red blood cell region using the valley and calculating MCV within the defined region. Alternatively, a curve fit of the white blood cell population on the red blood cell distribution histogram can be used to exclude the white blood cells. RBC can be corrected by subtracting the white blood cell concentration obtained from the analysis of a second aliquot sample, when the white blood cell concentration (WBC) exceeds a predetermined criterion. Hct of the blood sample can be calculated using the obtained MCV and RBC.

US Patent:

8192995, Jun 5, 2012

Filed:

Nov 11, 2009

Appl. No.:

12/616273

Inventors:

Shuliang Zhang - Miami FL, US
Jiuliu Lu - Homestead FL, US
Min Zheng - Pembroke Pines FL, US
Eric M. Grace - Cooper City FL, US
Jing Li - Miami FL, US
Maritza Lavernia - Miami FL, US
Ted W. Britton - Sunrise FL, US

Assignee:

Beckman Coulter, Inc. - Brea CA

International Classification:

G01N 33/72
G01N 33/48

US Classification:

436 66, 436 8, 436 10, 436 63, 436164, 436174, 436175, 436149, 436150, 422 8202, 422 8205, 422 8209, 422 73, 435 2, 435 29

Abstract:

A method of correction of particle interference to hemoglobin measurement of a blood sample on a hematology analyzer is provided. The method includes mixing an aliquot of a blood sample with a lytic reagent to lyse red blood cells and forming a sample mixture; measuring absorbance of the sample mixture at a predetermined wavelength of a hemoglobin chromogen formed in the sample mixture, and obtaining an apparent hemoglobin concentration of the blood sample using obtained absorbance; measuring concentration and size of cellular particles remaining in the sample mixture; removing contribution of the cellular particles to the apparent hemoglobin concentration using the concentration and the size of the cellular particles to obtain a corrected hemoglobin concentration of the blood sample; and reporting the corrected hemoglobin concentration of the blood sample.

US Patent:

20030032027, Feb 13, 2003

Filed:

Feb 12, 2002

Appl. No.:

10/073123

Inventors:

Jing Li - Flushing NY, US
Scott Powers - Greenlawn NY, US

International Classification:

C12Q001/68
G06F019/00
A61K048/00

US Classification:

435/006000, 702/020000, 514/044000

Abstract:

There are disclosed methods and compositions for the diagnosis, prevention, and treatment of tumors and cancers in mammals, for example, humans, utilizing the WIP1 gene, which are amplified breast, and/or lung, and/or colon, and/or ovarian, and/or prostate cancer genes. The WIP1 gene, its expressed protein products and antibodies are used diagnostically or as targets for cancer therapy; they are also used to identify compounds and reagents useful in cancer diagnosis, prevention, and therapy.