Jing J Li

US Patent:

6410330, Jun 25, 2002

Filed:

Jul 27, 2001

Appl. No.:

09/917533

Inventors:

Yi Li - Miami FL
Jing Li - Miami FL
Ted W. Britton - Sunrise FL

Assignee:

Coulter International Corp. - Miami FL

International Classification:

G01N 3100

US Classification:

436 10, 436 8, 436 17, 436 63, 436149, 436150, 436164, 422 73, 422 8201, 422 8202, 422 8209, 435 2, 435 29, 435 34, 435 39

Abstract:

A method for differentiating and enumerating nucleated red blood cells in a blood sample is described. The method includes the steps of lysing red blood cells of a blood sample with a lytic reagent, measuring nucleated blood cells by DC impedance measurement in a non-focused flow aperture, differentiating nucleated red blood cells from other cell types, and reporting nucleated red blood cells in the blood sample. The method further includes subtracting nucleated red blood cells and other interference materials from the count of remaining blood cells, and reporting a corrected white blood cell count of the blood sample. Additionally, the method further includes measuring spectrophotometric absorbance of the sample mixture at a predetermined wavelength of a hemoglobin chromogen formed upon lysing the blood sample, and reporting hemoglobin concentration of the blood sample.

US Patent:

6444439, Sep 3, 2002

Filed:

Jan 13, 1998

Appl. No.:

09/006428

Inventors:

Jing Li - Malden MA
Kazuhisa Nishizawa - Brookline MA
Wengian An - Framingham MA
Ellis L. Reinherz - Lincoln MA

Assignee:

Dana-Farber Cancer Institute - Boston MA

International Classification:

C12P 2106

US Classification:

435 691, 4352523, 536 231, 536 235, 530352

Abstract:

A human CD2 cytoplasmic tail binding protein, CD2BP1, is described, as well as the nucleic acids encoding the protein. Also described are expression vectors and recombinant host cells comprising nucleic acids encoding the CD2BP1 protein, and methods of use for the CD2BP1 protein and nucleic acids encoding the CD2BP1 protein.

US Patent:

6573102, Jun 3, 2003

Filed:

Jul 27, 2001

Appl. No.:

09/917530

Inventors:

Yi Li - Miami FL
Jing Li - Miami FL

Assignee:

Coulter International Corp. - Miami FL

International Classification:

G01N 3100

US Classification:

436 17, 436 8, 436 10, 436 18, 436 63, 2524081, 435 2, 435 29

Abstract:

A lytic reagent composition for measuring nucleated blood cells in a blood sample is described. The lytic reagent composition contains a quaternary ammonium surfactant, an ethoxylated phenol, and an ethoxylated alcohol. When mixed with a blood sample, the lytic reagent composition lyses red blood cells and enables a differentiation of nucleated red blood cells from other cell types by DC impedance measurement. The lytic reagent composition can further contain an organic ligand for determining total hemoglobin concentration of a blood sample photometrically. Further disclosed is a lytic reagent system including the lytic reagent composition and a diluent. In addition, a single reagent composition containing salts is also disclosed, which can be used without a separate diluent. The lytic reagent compositions can be used for concurrent measurement of nucleated red blood cells, WBC, and hemoglobin of a blood sample.

US Patent:

6673618, Jan 6, 2004

Filed:

Aug 23, 2002

Appl. No.:

10/226800

Inventors:

Yi Li - Miami FL
Jing Li - Miami FL
Ted W. Britton - Sunrise FL

Assignee:

Coulter International Corp. - Miami FL

International Classification:

G01N 3100

US Classification:

436 10, 436 8, 436 17, 436 63, 436149, 436150, 436164, 422 73, 422 8201, 422 8202, 422 8209, 435 2, 435 29, 435 34, 435 39

Abstract:

A method for differentiating and enumerating nucleated red blood cells in a blood sample is described. The method includes the steps of lysing red blood cells of a blood sample with a lytic reagent, measuring nucleated blood cells by DC impedance measurement in a non-focused flow aperture, differentiating nucleated red blood cells from other cell types, and reporting nucleated red blood cells in the blood sample. The method further includes subtracting nucleated red blood cells and other interference materials from the count of remaining blood cells, and reporting a corrected white blood cell count of the blood sample. Additionally, the method further includes measuring spectrophotometric absorbance of the sample mixture at a predetermined wavelength of a hemoglobin chromogen formed upon lysing the blood sample, and reporting hemoglobin concentration of the blood sample.

US Patent:

6689868, Feb 10, 2004

Filed:

Jul 13, 2000

Appl. No.:

09/615387

Inventors:

Jing Li - Lynnfield MA
Kazuhisa Nishizawa - Tokyo, JP
Wenqian An - Wayland MA
Ellis L. Reinherz - Lincoln MA

Assignee:

Dana-Farber Cancer Institute, Inc. - Boston MA

International Classification:

C07K 1600

US Classification:

5303879

Abstract:

A human CD2 cytoplasmic tail binding protein, CD2BP1, is described, as well as the nucleic acids encoding the protein. Also described are expression vectors and recombinant host cells comprising nucleic acids encoding the CD2BP1 protein, and methods of use for the CD2BP1 protein and nucleic acids encoding the CD2BP1 protein.

US Patent:

6916658, Jul 12, 2005

Filed:

Feb 2, 2004

Appl. No.:

10/770193

Inventors:

Jing Li - Miami FL, US
Yi Li - Miami FL, US

Assignee:

Beckman Coulter, Inc. - Fullerton CA

International Classification:

G01N031/00
G01N033/48

US Classification:

436 10, 436 8, 436 17, 436 63, 436149, 436150, 422 73, 422 8201, 422 8202, 435 2, 435 29, 435 34, 435 39

Abstract:

A method for measuring immature granulocytes in a blood sample is described. The method includes the steps of lysing red blood cells of a blood sample with a lytic reagent, analyzing the sample mixture by DC impedance measurement, determining immature granulocytes from obtained DC histogram, and reporting immature granulocytes in the blood sample. The method further includes measuring nucleated red blood cells in the blood sample.

US Patent:

7091330, Aug 15, 2006

Filed:

Jun 1, 2001

Appl. No.:

09/873106

Inventors:

Ellis L. Reinherz - Lincoln MA, US
Christian Freund - Berlin, DE
Jing Li - Lynnfield MA, US
Kazuhisa Nishizawa - Tokyo, JP
Gerhard Wagner - Brookline MA, US

Assignee:

Dana-Farber Cancer Institute, Inc. - Boston MA
President and Fellows of Harvard College - Cambridge MA

International Classification:

C12P 21/02
C12N 15/12

US Classification:

536 235, 435 691, 435325, 4353201

Abstract:

A human CD2 cytoplasmic tail binding protein, CD2BP2, is described, as well as the nucleic acids encoding the protein. Also described are expression vectors and recombinant host cells comprising nucleic acids encoding the CD2BP2 protein, and methods of use for the CD2BP2 protein and nucleic acids encoding the CD2BP2 protein.

US Patent:

7198953, Apr 3, 2007

Filed:

Oct 7, 2004

Appl. No.:

10/960370

Inventors:

Nery Ortiz - Miami FL, US
Jing Li - Miami FL, US
Yi Li - Miami FL, US
Sandra Socarras - Miami FL, US

Assignee:

Beckman Coulter, Inc. - Fullerton CA

International Classification:

G01N 33/48
G01N 31/00

US Classification:

436 63, 436 10, 436164, 436166

Abstract:

Methods of using a reference control composition containing a nucleated red blood cell component for measurement of nucleated red blood cells are disclosed. The nucleated red blood cell component is made of stabilized or processed nucleated blood cells which have impedance and optical properties simulating the impedance and optical properties of human nucleated red blood cells under a blood lysing condition as measured by a specific measurement. The methods include mixing the reference control composition with a lytic reagent, and analyzing the control sample mixture on a flow cytometric instrument by impedance, impedance and optical measurement, or DC impedance and a multi-dimensional measurement of light scatter, radio frequency and a second DC impedance; and reporting the simulated nucleated red blood cells in the reference control composition.