Jing J Lin

Molecule biology: Plasmid purification • genomic DNA and RNA isolation and quanti... • Gateway cloning • subcloning • plasmid/retroviral vector construction • DNA recombination • PCR (overlap PCR • real-time PCR • RT-PCR) • Site-directed mutagenesis • DNA restriction analysis • Co-immunoprecipitations • Electromobility shift assay (EMSA) • SDS-PAGE • Western blotting • Southern blot • Northern blot • North-westerns • chromatin immunoprecipitation (ChIP) • genotyping. Cell biology: Cell culture (... • hematopoietic cells • primary mouse embryonic fibroblasts (MEF) • primary hepatocytes cells isolation and ... • cancer cells and insect cells) • freezing/thawing • characterizing • and differentiating primary mouse cells.... • stable production cell lines development • gene overexpression • gene silencing through antisense express... • dual-luciferase assay • CAT assay • MTT colorimetric assay • Internucleosomal fragmentation assay. Pr... • affinity chromatography • hydrophobic interaction (HIC) • ion exchange (IEX) • reversed phase chromatography (RP) and s... • lentivirus production and titration • virus isolation • propagation • characterization • purification and titration • viral neutralization assay such as Plaqu... • Micro-cytopathic effect neutralization t... • Flow cytometry (FACS); antibody producti... • antibody and antibody titer characteriza... • label antibody by FITC and HRP • Cytotoxic T lymphocyte (CTL) • lymphocyte transformation test • immunofluorescence staining • IFA • ELISA • immunocytochemistry • immunohistochemistry • hemagglutination assay (HA) & hemagg... • liposome preparation and test. Instrumen... • Zeiss LSM 510 & LSM 710 for confocal... • fluorescent microscope • real-time PCR machine • spectrophotometers • spectrofluorometer • sonication devices and ultracentrifuges ...