Jingyi Li

US Patent:

20130203045, Aug 8, 2013

Filed:

May 26, 2011

Appl. No.:

13/699983

Inventors:

James P. Landers - Charlottesville VA, US
Kimberly A. Kelly - Crozet VA, US
Jingyi Li - Charlottesville VA, US
Daniel C. Leslie - Brookline MA, US

Assignee:

University of Virginia Patent Foundation - Charlottesville VA

International Classification:

C12Q 1/68
C12Q 1/70

US Classification:

435 5, 435 612, 435 61, 435 611

Abstract:

The invention provides methods to detect or determine the presence or amount of a pathogen, such as a virus or bacterium, in a sample or the amount of cells based on the detection of their genomic DNA. The method employs magnetic substrates and subjects the sample and the magnetic substrate to forms of energy so as to induce aggregate formation and detects the aggregates.

US Patent:

20120149587, Jun 14, 2012

Filed:

May 26, 2011

Appl. No.:

13/116659

Inventors:

James P. Landers - Charlottesville VA, US
Jingyi Li - Charlottesville VA, US
Daniel C. Leslie - Brookline MA, US

Assignee:

University of Virginia Patent Foundation - Charlottesville VA

International Classification:

C40B 30/00
C12Q 1/68

US Classification:

506 7, 435 61, 435 612

Abstract:

The invention provides methods to detect or quantify cells such as nucleated cells in a sample such as a physiological sample, which employ magnetic substrates and subjects the sample and the magnetic substrate to forms of energy so as to induce aggregate formation.

US Patent:

20230072999, Mar 9, 2023

Filed:

Aug 5, 2022

Appl. No.:

17/881802

Inventors:

- Southampton, GB
Christopher Birch - Charlottesville VA, US
Daniel Mills - Charlottesville VA, US
Brian Root - Charlottesville VA, US
James Landers - Charlottesville VA, US
Jingyi Li - Charlottesville VA, US
Matthew Yeung - Mount Waverley, AU
David Saul - Dunedin, AU
David Vigil - Mount Waverley, AU
Andrew Guy - Mount Waverley, AU
Stan Wada - Mount Waverley, AU
Betina De Gorordo - Mount Waverley, AU
Steward Dodman - Mount Waverley, AU
Tom Moran - Southampton, GB
Stuart Knowles - Mount Waverley, AU
Fernando Dias - Mount Waverley, AU
Rick Gardner - Mount Waverley, AU

International Classification:

B01L 3/00
G01N 33/569
G01N 33/543
G01N 1/18
G01N 1/40
B01L 7/00

Abstract:

A collection device for a biological sample to capture target compounds such as viruses or other pathogens or particles for testing from within the sample and move the captured target compound to a separate chamber for subsequent processing. The collection device can include an openable substance blister including capture particles located in a cup interior. Capture particles can attract and bind the target compounds from the sample. An extraction tube extracts any nucleic acid from the target compound for storage or subsequent amplification and testing to confirm presence of known microorganisms. The extraction tube can comprise a heat-deformable material and can be connected to a microfluidic cartridge for further processing of nucleic acid including, amplification and detection. The microfluidic cartridge includes valves and a plurality of chambers for amplification.

US Patent:

20190054468, Feb 21, 2019

Filed:

Oct 21, 2016

Appl. No.:

15/770413

Inventors:

- Charlottesville VA, US
Kimberly Renee JACKSON - Atlanta GA, US
Daniel MILLS - Charlottesville VA, US
Gavin T. GARNER - Charlottesville VA, US
Jacquelyn A. DuVall - Raleigh NC, US
Jingyi LI - Charlottesville VA, US

International Classification:

B01L 3/00
C12Q 1/6809
C12Q 1/6816
C12Q 1/6827
G01N 33/52
G01N 33/543
G01N 33/68
G01N 35/00

Abstract:

This disclosure provides for apparatuses, systems and methods for in vitro sample detection. For example in one embodiment, this disclosure provides an automated Pe-toner microfluidic device (and related method) on a centrifugal platform for DNA sample lysis and DNA extraction. A second embodiment provides a system and method for qualitative detection, quantification, and real-time monitoring of nucleic acid amplification products using magnetic bead aggregation inhibition. A third embodiment provides a platform for simultaneous detection of mRNA markers from blood, cell-free semen, sperm, saliva, and vaginal fluid. The third embodiment comprises a system and method that provide for simple, rapid, and fluorescence-free detection of body fluids using mRNA marker amplification and optical detection for mRNA marker analysis with a smart phone with image analysis.

US Patent:

20180304253, Oct 25, 2018

Filed:

Oct 13, 2016

Appl. No.:

15/768115

Inventors:

- Charlottesville VA, US
Jacquelyn A. DuVall - Raleigh NC, US
Delphine Le Roux - Barboursville VA, US
Brian Root - Charlottesville VA, US
Daniel MIlls - Charlottesville VA, US
Daniel A. Nelson - Charlottesville VA, US
An-chi Tsuei - Charlottesville VA, US
Brandon L. Thompson - Charlottesville VA, US
Jingyi Li - Charlottesville VA, US
Christopher Birch - Charlottesville VA, US

International Classification:

B01L 3/00
B01L 7/00
G01N 27/447

Abstract:

A method to extract, amplify and separate nucleic acid in a microfluidic device having a plurality of chambers and channels can include a) introducing cells having nucleic acid to a first chamber of the microfluidic device and subjecting the cells in the first chamber to conditions that lyse the cells. The method can further include b) subjecting the first chamber to centrifugal force, thereby allowing the lysate or a portion thereof having nucleic acid to be distributed to a second chamber through a first channel in the microfluidic device. The method can also include c) combining the lysate or the portion thereof and reagents for amplification of the nucleic acid, thereby providing a second mixture. The method can also include d) subjecting the second chamber to centrifugal force, thereby allowing gas to be expelled from the second mixture.

US Patent:

20150093838, Apr 2, 2015

Filed:

Oct 1, 2014

Appl. No.:

14/503955

Inventors:

James P. Landers - Charlottesville VA, US
Yiwen Ouyang - Charlottesville VA, US
Jingyi Li - Charlottesville VA, US

International Classification:

B01L 3/00
B32B 38/00
B32B 38/10
B32B 37/18

US Classification:

436180, 422503, 156182

Abstract:

A microfluidic device having a chip defining fluid channels and having toner patches printed within the channels. The toner patches are printed with hydrophobic toner to apply inertial pressure to fluids travelling through the channels. The density of hydrophobic toner and the dimensions of the toner patch can be varied to alter the inertial pressure applied to the fluid. The chip can be rotated about a rotational axis to apply external pressure to fluids sufficient to overcome the inertial pressure created by the toner patch to push fluid past the toner patch. The rotational speed of the chip can be varied to facilitate movement of fluid through the channels and to push fluid past the hydrophobic toner patches.