Joan Margaret Zakel

US Patent:

7399589, Jul 15, 2008

Filed:

Dec 11, 2003

Appl. No.:

10/735082

Inventors:

James G. Lazar - Bethesda MD, US
Joan M. Zakel - Ellicott City MD, US
Christina M. Strange - Frederick MD, US
Inna R. Williams - Rockville MD, US
Attila T. Lorincz - North Potomac MD, US
Abel De La Rosa - Alpharetta GA, US

Assignee:

Digene Corporation - Gaithersburg MD

International Classification:

C12Q 1/68
C12P 21/08
C07H 21/04

US Classification:

435 6, 5303881, 53038821, 536 243

Abstract:

The present invention provides a hybridization method and kit for the detection and measurement of biological molecules. A test sample containing the biological molecules of interest is hybridized with an unlabeled or optionally a detectably-labeled complementary biomolecule to form a double-stranded hybrid immobilized to a solid phase. The immobilized hybrid may be detected with an entity which specifically recognizes an RNA:DNA hybrid, followed by analyses and quantification. Therefore, the present invention provides a method and kit to detect and measure biological molecules that is simple to use, highly specific, sensitive, and accurate for screening a plurality of biological molecules.

US Patent:

6686151, Feb 3, 2004

Filed:

Nov 6, 2000

Appl. No.:

09/707178

Inventors:

James G. Lazar - Bethesda MD
Joan M. Zakel - Ellicott City MD
Christina M. Strange - Germantown MD
Inna R. Williams - Rockville MD
Attila T. Lorincz - North Potomac MD
Abel De La Rosa - Alpharetta GA

Assignee:

Digene Corporation - Gaithersburg MD

International Classification:

C12Q 168

US Classification:

435 6, 435 71, 53038821, 536 243

Abstract:

The present invention provides a hybridization method and kit for the detection and measurement of biological molecules. A test sample containing the biological molecules of interest is hybridized with an unlabeled or optionally a detectably labeled complementary biomolecule to form a double-stranded hybrid immobilized to a solid phase. The immobilized hybrid may be detected with an entity which specifically recognizes an RNA:DNA hybrid, followed by analyses and quantification. Therefore, the present invention provides a method and kit to detect and measure biological molecules that is simple to use, highly specific, sensitive, and accurate for screening a plurality of biological molecules.