Joe Z Martinez

US Patent:

20230076395, Mar 9, 2023

Filed:

Aug 16, 2022

Appl. No.:

17/820232

Inventors:

- Pasadena CA, US
Michael B. Elowitz - Pasadena CA, US
Joe A. Martinez - Pasadena CA, US

International Classification:

C12N 15/88
C12N 15/62
C12N 15/86
C07K 14/005

Abstract:

Disclosed herein include methods, compositions, and kits suitable for use in the delivery of polyribonucleotides and circuits. There are provided, in some embodiments, RNA exporter proteins comprising an RNA-binding domain, a membrane-binding domain, and an interaction domain capable of nucleating self-assembly. Disclosed herein include polynucleotides encoding cargo RNA molecule(s). In some embodiments, a plurality of RNA exporter proteins are capable of self-assembling into lipid-enveloped nanoparticles (LNs) secreted from a sender cell in which the RNA exporter proteins are expressed, thereby generating a population of LNs comprising a fusogen and exported cargo RNA molecule(s).