Joel Allen Jessee

US Patent:

6399334, Jun 4, 2002

Filed:

Sep 23, 1998

Appl. No.:

09/159496

Inventors:

Paul E. Nisson - Gaithersburg MD
Joel Jessee - Mount Airy MD

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12P 1934

US Classification:

435 911, 435 6

Abstract:

The present invention relates generally to methods for producing normalized nucleic acid libraries in which each member of the library can be isolated with approximately equivalent probability. In particular, the present methods comprise subtractive hybridization of a nucleic acid library with haptenylated (e. g. , biotinylated, avidinated or streptavidinated) nucleic acid molecules that are complementary to one or more of the nucleic acid molecules of the library, such that the variation in the abundances of the individual nucleic acid molecules in the library is reduced. The invention also relates to production of normalized nucleic acid libraries (particularly cDNA libraries) in which contaminating nucleic acid molecules have been reduced or eliminated, and to normalized nucleic acid libraries produced by such methods.

US Patent:

6451579, Sep 17, 2002

Filed:

Jul 28, 1999

Appl. No.:

09/361740

Inventors:

Joel A. Jessee - Mount Airy MD
Valentina C. Ciccarone - Gaithersburg MD

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12N 700

US Classification:

4352351, 435440, 435455, 435 6, 4353201, 435 691, 435 15, 424945, 514 44, 530350, 530435, 530194

Abstract:

The present invention describes cells and constructs for a regulated viral (e. g. alphavirus) expression system, where gene expression is controlled by controlling expression of replicases or nonstructural proteins and/or controlling the amount of such proteins introduced in a cell, which in turn regulates RNA replication and subsequently gene expression. Particularly, this system takes advantage of the high level expression of the alphavirus systems for recombinant protein production and allows for large scale applications without biosafety concerns.

US Patent:

6787305, Sep 7, 2004

Filed:

Mar 12, 1999

Appl. No.:

09/266935

Inventors:

Joel A. Jessee - Mount Airy MD
David Schuster - Poolesville MD
Jiulin Xia - Germantown MD
Gulilat Gebeyehu - Potomac MD

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12Q 168

US Classification:

435 6, 435 911, 435 912, 536 221, 536 231, 536 253

Abstract:

The present invention is directed to compositions and methods for enhancing synthesis of nucleic acid molecules, particularly GC-rich nucleic acid molecules. Specifically, the invention provides compositions comprising one or more nitrogen-containing organic compounds having a formula selected from the group consisting of formula I and formula II (or salts or derivatives thereof), preferably 4-methylmorpholine N-oxide or betaine (carboxymethyltrimethylammonium), and further comprising one or more compounds selected from the group consisting of proline and an N-alkylimidazole compound, and more preferably proline, 1-methyliimidazole or 4-methylimidazole. The invention further relates to methods for enhanced, high-fidelity synthesis of nucleic acid molecules, including via amplification (particularly PCR), reverse transcription, and sequencing methods. The invention also relates to nucleic acid molecules synthesized by these methods, to fragments or derivatives thereof, and to vectors and host cells comprising such nucleic acid molecules, fragments, or derivatives. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the compositions of the invention.

US Patent:

6960464, Nov 1, 2005

Filed:

Apr 11, 2002

Appl. No.:

10/120163

Inventors:

Joel A. Jessee - Mount Airy MD, US
Fredric R. Bloom - Germantown MD, US
Thuan Trinh - Gaithersburg MD, US

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12N001/20
A01N063/00

US Classification:

4352521, 4352528, 435260, 435849, 424 934, 424 9348

Abstract:

This invention relates to a method for producing cells which are competent for transformation and which may be stably stored for extended periods of time at various temperatures. The method involves growing cells in a growth conducive medium, rendering said cells competent, and lyophilizing said competent cells. The invention further relates to competent cells produced by such a method, to methods of transforming said cells with a DNA molecule, and to a method of producing a desired protein or polypeptide from said transformed cells.

US Patent:

7344863, Mar 18, 2008

Filed:

Jul 27, 2004

Appl. No.:

10/899139

Inventors:

Joel A. Jessee - Mount Airy MD, US
David Schuster - Poolesville MD, US
Jiulin Xia - Germantown MD, US
Gulilat Gebeyehu - Potomac MD, US

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12P 21/00
C12P 19/34

US Classification:

435 701, 435 911

Abstract:

The present invention is directed to compositions and methods for enhancing synthesis of nucleic acid molecules, particularly GC-rich nucleic acid molecules. Specifically, the invention provides compositions comprising one or more nitrogen-containing organic compounds having a formula selected from the group consisting of formula I and formula II (or salts or derivatives thereof), preferably 4-methylmorpholine N-oxide or betaine (carboxymethyltrimethylammonium), and further comprising one or more compounds selected from the group consisting of proline and an N-alkylimidazole compound, and more preferably proline, 1-methylimidazole or 4-methylimidazole. The invention further relates to methods for enhanced, high-fidelity synthesis of nucleic acid molecules, including via amplification (particularly PCR), reverse transcription, and sequencing methods. The invention also relates to nucleic acid molecules synthesized by these methods, to fragments or derivatives thereof, and to vectors and host cells comprising such nucleic acid molecules, fragments, or derivatives. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the compositions of the invention.

US Patent:

7648832, Jan 19, 2010

Filed:

Aug 31, 2005

Appl. No.:

11/214800

Inventors:

Joel A. Jessee - Mount Airy MD, US
Fredric R. Bloom - Germantown MD, US
Thuan Trinh - Gaithersburg MD, US

Assignee:

Life Technologies, Corp. - Carlsbad CA

International Classification:

C12N 1/20
A01N 63/00

US Classification:

435260, 4352521, 4352522, 435849, 424 934, 424 9348

Abstract:

This invention relates to a method for producing cells which are competent for transformation and which may be stably stored for extended periods of time at various temperatures. The method involves growing cells in a growth conducive medium, rendering said cells competent, and lyophilizing said competent cells. The invention further relates to competent cells produced by such a method, to methods of transforming said cells with a DNA molecule, and to a method of producing a desired protein or polypeptide from said transformed cells.

US Patent:

7915230, Mar 29, 2011

Filed:

May 17, 2006

Appl. No.:

11/434765

Inventors:

Joel Jessee - Mount Airy MD, US
Gulilat Gebeyehu - Potomac MD, US

Assignee:

Molecular Transfer, Inc. - Gaithersburg MD

International Classification:

A61K 48/00
A61K 38/16
C12N 15/00

US Classification:

514 44R, 435455, 530358

Abstract:

Compositions and methods for improved delivery of macromolecules into eukaryotic cells are provided. Fusogenic peptides from fusion proteins of non-enveloped viruses enhance the efficiency of transfection of eukaryotic cells mediated by transfection agents such as cationic lipids, polycationic polymers such as PEI and dendrimers. These fusogenic peptides are used as part of a transfection complex that efficiently delivers a macromolecule, for example, a nucleic acid, into a eukaryotic cell. Novel cationic lipids and compositions of cationic lipids also are provided that may be used for the introduction of macromolecules such as nucleic acids, proteins and peptides into a variety of cells and tissues. The lipids can be used alone, in combination with other lipids and/or in combination with fusogenic peptides to prepare transfection complexes.

US Patent:

8034977, Oct 11, 2011

Filed:

Apr 27, 2009

Appl. No.:

12/430747

Inventors:

Gulilat Gebeyehu - Potomac MD, US
Taysir M. Jaouni - Hagerstown MD, US
Joel Jessee - Mount Airy MD, US

Assignee:

Molecular Transfer, Inc. - Gaithersburg MD

International Classification:

C07C 211/63
A61K 31/14
C12N 15/88

US Classification:

564292, 564294, 564295, 514642, 435458

Abstract:

Cationic lipid compositions are provided that are useful for efficient delivery of macromolecules, such as nucleic acids, into a wide variety of eukaryotic cell types. Methods for using the compositions also are provided.