John Richard Glass

US Patent:

20200172877, Jun 4, 2020

Filed:

Sep 26, 2019

Appl. No.:

16/583390

Inventors:

- Berkshire, GB
- La Jolla CA, US
Pirada SUPHAPHIPHAT - Cambridge MA, US
Daniel GIBSON - La Jolla CA, US
David WENTWORTH - La Jolla CA, US
Timothy STOCKWELL - La Jolla CA, US
John GLASS - La Jolla CA, US

Assignee:

Seqirus UK Limited - Berkshire
Synthetic Genomics, Inc. - La Jolla CA

International Classification:

C12N 7/00
C12N 5/071
A61K 39/145
C07K 14/005

Abstract:

Improved methods for the production of reassortant influenza viruses are provided.

US Patent:

20150353900, Dec 10, 2015

Filed:

Jan 23, 2014

Appl. No.:

14/762439

Inventors:

- Basel, CH
- La Jolla CA, US
Pirada SUPHAPHIPHAT - Cambridge MA, US
Daniel GIBSON - La Jolla CA, US
David WENTWORTH - La Jolla CA, US
Timothy STOCKWELL - La Jolla CA, US
John GLASS - La Jolla CA, US

Assignee:

NOVARTIS AG - Basel
SYNTHETIC GENOMICS VACCINES, INC. - La Jolla CA

International Classification:

C12N 7/00
A61K 39/145
C12N 5/071

Abstract:

Improved methods for the production of reassortant influenza viruses are provided.

US Patent:

20150037890, Feb 5, 2015

Filed:

Jul 31, 2014

Appl. No.:

14/449106

Inventors:

James Pelletier - Cambridge MA, US
Neil Gershenfeld - Cambridge MA, US
John Glass - Germantown MD, US
Elizabeth Strychalski - North Potomac MD, US

Assignee:

Massacusetts Institute of Technology - Cambridge MA

International Classification:

C12N 15/04
C12M 1/42
C12N 15/87

US Classification:

435440, 4352851

Abstract:

In exemplary implementations, transplantation of nucleic acids into cells occurs in microfluidic chambers. The nucleic acids may be large nucleic acid molecules with more than 100 kbp. In some cases, the microfluidic chambers have only one orifice that opens to a flow channel. In some cases, flow through a microfluidic chamber temporarily ceases due to closing one or more valves. Transplantation occurs during a period in which the contents of the chambers are shielded from shear forces. Diffusion, centrifugation, suction from a vacuum channel, or dead-end loading may be used to move cells or buffers into the chambers.