John F Kramarczyk

US Patent:

20200188481, Jun 18, 2020

Filed:

Sep 27, 2019

Appl. No.:

16/585357

Inventors:

- Cambridge MA, US
Brett Boghigian - Boston MA, US
Caitlyn Harvey - Wilmington MA, US
David Berry - Brookline MA, US
David Young - Portsmouth NH, US
Geoffrey Von Maltzahn - Boston MA, US
John Kramarczyk - Somerville MA, US
Jameson Rogers - Cambridge MA, US
Kathryn Heard - Watertown MA, US
Michael Doherty - Somerville MA, US
Nathaniel Silver - Cambridge MA, US
Phillip Samayoa - Cambridge MA, US
Subhayu Basu - Chestnut Hill MA, US
Shaila Rahman - Cambridge MA, US
Vimal Vaidya - Watertown MA, US

International Classification:

A61K 38/17
G16B 20/00
A61K 38/00
A23L 33/00
A23L 33/17
A23L 33/18
A23L 33/175
A61K 38/38
A61K 9/00
A61K 9/14
A61K 9/20
A61K 9/28
G01N 33/68
A23L 2/66
A61K 38/16
A61K 45/06
A23L 33/195
A61K 38/10
A61K 38/45

Abstract:

Nutritive polypeptides are provided herein. Also provided are various other embodiments including nucleic acids encoding the polypeptides, recombinant microorganisms that make the polypeptides, vectors for expressing the polypeptides, methods of making the polypeptides using recombinant microorganisms, compositions and formulations that comprise the polypeptides, and methods of using the polypeptides, compositions and formulations.

US Patent:

20200109175, Apr 9, 2020

Filed:

Sep 10, 2019

Appl. No.:

16/566734

Inventors:

- Cambridge MA, US
Brett Adam Boghigian - Boston MA, US
Nathaniel W. Silver - Cambridge MA, US
Geoffrey von Maltzahn - Boston MA, US
Michael Hamill - Wellesley MA, US
Rajeev Chillakuru - Cambridge MA, US
John F. Kramarczyk - Somerville MA, US

International Classification:

C07K 14/00
A61K 31/198
A23L 33/18
A23J 3/04
C07K 14/415
C12N 15/82
A61K 38/16
A23L 33/00
A23L 33/17

Abstract:

Charged nutritive proteins are provided. In some embodiments the nutritive proteins an aqueous solubility of at least 12.5 g/L at pH 7. In some embodiments the nutritive proteins an aqueous solubility of at least 50 g/L at pH 7. In some embodiments the nutritive proteins an aqueous solubility of at least 100 g/L at pH 7. In some embodiments the nutritive proteins comprise at least one of a level of a) a ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of branch chain amino acid residues to total amino acid residues present in a benchmark protein; b) a ratio of leucine residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of leucine residues to total amino acid residues present in a benchmark protein; and c) a ratio of essential amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of essential amino acid residues to total amino acid residues present in a benchmark protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things.

US Patent:

20170298102, Oct 19, 2017

Filed:

Feb 10, 2017

Appl. No.:

15/429442

Inventors:

- Cambridge MA, US
Michael J. Hamill - Wellesley MA, US
Rajeev Chillakuru - Cambridge MA, US
John F. Kramarczyk - Somerville MA, US
David Arthur Berry - Brookline MA, US
Brett Adam Boghigian - Boston MA, US
Nathaniel W. Silver - Cambridge MA, US

International Classification:

C07K 14/415
A23L 33/175
A23J 3/00
A61K 38/00

Abstract:

Nutritive proteins are provided. In some embodiments the nutritive proteins comprise at least one of a level of a) a ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of branch chain amino acid residues to total amino acid residues present in whey protein; b) a ratio of leucine residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of leucine residues to total amino acid residues present in whey protein; and c) a ratio of essential amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of essential amino acid residues to total amino acid residues present in whey protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things.

US Patent:

20170253639, Sep 7, 2017

Filed:

Feb 10, 2017

Appl. No.:

15/429412

Inventors:

- Cambridge MA, US
Brett Adam Boghigian - Boston MA, US
Nathaniel W. Silver - Cambridge MA, US
Geoffrey von Maltzahn - Boston MA, US
Rajeev Chillakuru - Cambridge MA, US
Michael Hamill - Wellesley MA, US
John F. Kramarczyk - Somerville MA, US

International Classification:

C07K 14/435
A23L 33/18

Abstract:

Nutritive proteins are provided. In some embodiments the nutritive proteins comprise a first polypeptide sequence comprising a fragment of a naturally-occurring nutritive protein. In some embodiments the fragment comprises at least one of a) an enhanced ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein; b) an enhanced ratio of leucine residues to total amino acid residues present in the nutritive protein; and c) an enhanced ratio of essential amino acid residues to total amino acid residues present in the nutritive protein. In some embodiments, the fragment comprises at least one of a) a ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein; b) a ratio of leucine residues to total amino acid residues present in the nutritive protein; and c) a ratio of essential amino acid residues to total amino acid residues present in the nutritive protein, that is equal to or greater than the corresponding ratio present in a benchmark protein such as whey, egg or soy protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things.

US Patent:

20170246244, Aug 31, 2017

Filed:

Feb 10, 2017

Appl. No.:

15/429420

Inventors:

- Cambridge MA, US
Brett Adam Boghigian - Boston MA, US
Nathaniel W. Silver - Cambridge MA, US
Geoffrey von Maltzahn - Boston MA, US
Michael Hamill - Wellesley MA, US
Rajeev Chillakuru - Cambridge MA, US
John F. Kramarczyk - Somerville MA, US

International Classification:

A61K 38/16
A61K 31/198
A23L 33/00
A23J 3/04
A23L 33/17

Abstract:

Charged nutritive proteins are provided. In some embodiments the nutritive proteins an aqueous solubility of at least 12.5 g/L at pH 7. In some embodiments the nutritive proteins an aqueous solubility of at least 50 g/L at pH 7. In some embodiments the nutritive proteins an aqueous solubility of at least 100 g/L at pH 7. In some embodiments the nutritive proteins comprise at least one of a level of a) a ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of branch chain amino acid residues to total amino acid residues present in a benchmark protein; b) a ratio of leucine residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of leucine residues to total amino acid residues present in a benchmark protein; and c) a ratio of essential amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of essential amino acid residues to total amino acid residues present in a benchmark protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things.

US Patent:

20160339078, Nov 24, 2016

Filed:

Mar 25, 2016

Appl. No.:

15/081004

Inventors:

- Cambridge MA, US
Brett Boghigian - Boston MA, US
Caitlyn Harvey - Wilmington MA, US
David Berry - Brookline MA, US
David Young - Portsmouth NH, US
Geoffrey Von Maltzahn - Boston MA, US
John Kramarczyk - Somerville MA, US
Jameson Rogers - Cambridge MA, US
Kathryn Heard - Watertown MA, US
Michael Doherty - Somerville MA, US
Nathaniel Silver - Cambridge MA, US
Phillip Samayoa - Cambridge MA, US
Subhayu Basu - Chestnut Hill MA, US
Shaila Rahman - Cambridge MA, US
Vimal Vaidya - Watertown MA, US

International Classification:

A61K 38/16
A61K 38/10
A23L 33/195
A61K 38/45
G01N 33/68
A61K 9/00
A61K 38/17

Abstract:

Nutritive polypeptides are provided herein. Also provided are various other embodiments including nucleic acids encoding the polypeptides, recombinant microorganisms that make the polypeptides, vectors for expressing the polypeptides, methods of making the polypeptides using recombinant microorganisms, compositions and formulations that comprise the polypeptides, and methods of using the polypeptides, compositions and formulations.

US Patent:

20150126441, May 7, 2015

Filed:

Mar 15, 2013

Appl. No.:

14/387679

Inventors:

- Cambridge MA, US
Brett Adam Bohigian - Boston MA, US
Nathaniel W. Silver - Cambridge MA, US
Michael J. Hamill - Wellesley MA, US
Geoffrey von Maltzahn - Boston MA, US
John F. Kramarczyk - Somerville MA, US
Rajeev Chillakuru - Cambridge MA, US

International Classification:

A23J 1/00
A23L 1/29
A23L 1/305
C07K 14/47
C07K 14/415

US Classification:

514 55, 530328, 530327, 530326, 530325, 530324, 530350, 435 691, 4352523, 426656, 426 72, 426 61, 426 2

Abstract:

Nutritive proteins comprising no phenylalanine (Phe) are provided. In some embodiments the nutritive proteins comprise at least one of a level of a) a ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of branch chain amino acid residues to total amino acid residues present in a benchmark protein; b) a ratio of leucine residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of leucine residues to total amino acid residues present in a benchmark protein; and c) a ratio of essential amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of essential amino acid residues to total amino acid residues present a benchmark protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things. The compositions are useful, for example, to provide protein in the diet of subjects with a disorder characterized by accumulation of Phe in the body.

US Patent:

20150087602, Mar 26, 2015

Filed:

Mar 15, 2013

Appl. No.:

14/387675

Inventors:

- Cambridge MA, US
Michael J. Hamill - Wellesley MA, US
Rajeev Chillakuru - Cambridge MA, US
John F. Kramarczyk - Somerville MA, US
David Arthur Berry - Brookline MA, US
Brett Adam Boghigian - Boston MA, US
Nathaniel W. Silver - Cambridge MA, US

International Classification:

C07K 14/47
C07K 14/415

US Classification:

514 212, 530372, 4352521, 435 691, 530350, 530375, 530376

Abstract:

Nutritive proteins are provided. In some embodiments the nutritive proteins comprise at least one of a level of a) a ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of branch chain amino acid residues to total amino acid residues present in whey protein; b) a ratio of leucine residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of leucine residues to total amino acid residues present in whey protein; and c) a ratio of essential amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of essential amino acid residues to total amino acid residues present in whey protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things.