John Stewart Link

US Patent:

61747092, Jan 16, 2001

Filed:

Jan 31, 1994

Appl. No.:

8/221543

Inventors:

John H. Kenten - Gaithersburg MD
John R. Link - Springfield VA

Assignee:

IGEN International, Inc. - Gaithersburg MD

International Classification:

C12Q 168
C12P 1934

US Classification:

435 912

Abstract:

A process for exponentially amplifying a selected nucleic acid sequence present in a sample, comprising the steps of forming a mixture of the sample and a single primer designed to hybridize with the selected nucleic acid sequence; causing the single primer to hybridize to a single strand of the nucleic acid sequence of interest; forming a duplex product of the nucleic acid by a polymerase reaction; separating the duplex product into single strands; and repeating the preceding steps until the rate of production of the amplification product is exponential and the nucleic acid sequence of interest has been amplified.

US Patent:

56353472, Jun 3, 1997

Filed:

Jan 28, 1994

Appl. No.:

8/188943

Inventors:

John R. Link - Springfield VA
Satyanarayana R. Gudibande - Rockville MD
John H. Kenten - Gaithersburg MD

Assignee:

IGEN, Inc. - Gaithersburg MD

International Classification:

C12Q 168
C12P 1934
C07H 2104

US Classification:

435 6

Abstract:

A method of detecting a nucleic acid sequence of interest in the amplification product of a polymerase chain reaction or other primer directed reaction comprising the steps of: (a) incorporating in a polymerase chain reaction mixture or other primer directed reaction mixture at least one nucleic acid sequence complementary to said nucleic acid sequence of interest labeled (i) at the 3' end thereof, or (ii) at the 3' and the 5' end thereof with a compound capable of electrochemiluminescence; (b) conducting a polymerase chain reaction or other primer directed reaction; and (c) measuring the electrochemiluminescence of labeled amplification product.