John R O'Connell

US Patent:

20040175737, Sep 9, 2004

Filed:

Dec 19, 2003

Appl. No.:

10/741554

Inventors:

Matthew Olson - Bardonia NY, US
John O'Connell - Cedar Grove NJ, US

Assignee:

Wyeth - Madison NJ

International Classification:

C12Q001/68
C12P019/34

US Classification:

435/006000, 435/091200

Abstract:

The present invention provides a method of detecting a nuclease-mediated cleavage of a target nucleic acid through hybridizing a target nucleic acid to a fluorescently labeled oligonucleotide probe complementary to the target nucleic acid and containing a flourophor at one terminus and a quenching group at the other terminus. When the probe is unhybridized to the target nucleic acid, the probe adopts a conformation that places the flourophor and quencher in such proximity that the quencher quenches the flourescent signal of the flourophor and formation of the probe-target hybrid causes sufficient separation of the flourophor and quencher to reduce quenching of the flourescent signal of the flourophor. Once hybrized, the method contacts the probe-target hybrid with an agent having nuclease activity in an amount sufficient to selectively cleave the target nucleic acid and thereby release the intact probe. Detecting the release of the probe is then measured by following a decrease in the flourescent signal of the flourophor as compared to the signal of the probe-target hybrid.

US Patent:

20130160256, Jun 27, 2013

Filed:

Dec 23, 2011

Appl. No.:

13/336312

Inventors:

Charlton Joseph Waldman - Larchmont NY, US
Eric Lorberfeld - New Rochelle NY, US
John O'Connell - Milford CT, US

Assignee:

EASY STREET PRODUCTS, LLC - New York NY

International Classification:

A43C 7/04
A44B 99/00

US Classification:

247126

Abstract:

A lace or draw string fastening system can include a clamping unit and a capture element. The clamping unit can clamp onto the laces, such as shoe laces, to tighten the shoes and the capture element can capture and contain the free ends of the laces that extend from the clamping unit to prevent tripping by securing them to other portions of the laces or the article that houses the laces. The fastening system can be used in a shoe lace fastening system without the need for knot tying. The clamping unit can clamp the laces together at the top of the shoe to hold the laces tight while the capture element can contain or secure the free ends of the shoe lace to the shoe or the laces adjacent the toe to prevent tripping.

US Patent:

40392660, Aug 2, 1977

Filed:

Jul 19, 1976

Appl. No.:

5/706230

Inventors:

John W. O'Connell - Carmel NY

International Classification:

B23B 5100

US Classification:

408202

Abstract:

A longitudinal cutting tool is formed with at least one groove and a stop collar is received on the cutting tool. The stop collar has a stop surface, and yieldably resilient means in the form of a threaded screw passing through a slot in the collar, and rubberized buttons inserted into the collar are used to adjust the collar on the cutting tool, and to operatively connect it thereto so as not to damage its groove; the cutting depth of the tool is limited when the stop face engages with the stop surface.

US Patent:

58916210, Apr 6, 1999

Filed:

Sep 24, 1997

Appl. No.:

8/936646

Inventors:

Renee M. Chabin - Neptune NJ
David W. Kuo - Princeton NJ
John F. O'Connell - Cranbury NJ
David L. Pompliano - Lawrenceville NJ
Kenny K. Wong - Edison NJ

Assignee:

Merck & Co., Inc. - Rahway NJ

International Classification:

C12Q 100
C12Q 137
C12Q 118
C12Q 148

US Classification:

435 4

Abstract:

An in vitro screening assay which identifies enzyme inhibitors and allows for the simultaneous assay of many enzymes. Enzyme, substrate, co-factor, etc. concentrations are optimized so that inhibitors of any one of the enzymes in the pathway are equally likely to be detected. Necessarily, the flux of substrate through each enzyme should be nearly the same during the assay, i. e. , each of the enzyme catalyzed steps must be equally rate-limiting. Preferably, optimal assay conditions are predicted by computer modeling. Further, the pathway conditions are optimized through variation of enzyme, starting substrate, co-substrate and co-factor concentrations. A positive response is initially detected as a change in the amount of the product generated at the end of the enzyme cascade as compared to a standard. A sample producing a positive result can be deconvoluted.