John A Perkins

US Patent:

6551813, Apr 22, 2003

Filed:

May 6, 1999

Appl. No.:

09/306615

Inventors:

John B. Perkins - Reading MA
Alan Sloma - Watertown MA
Janice G. Pero - Lexington MA
Randolph T. Hatch - Wellesley MA
Theron Hermann - Framingham MA
Thomas Erdenberger - Arlington MA

Assignee:

Roche Vitamins Inc. - Parsippany NJ

International Classification:

C12P 2500

US Classification:

4352536, 435 66

Abstract:

Vectors and recombinant bacteria for overproducing riboflavin, in which nucleic acid overproducing riboflavin biosynthetic proteins is introduced in the chromosome of the host organism, e. g. at multiple sites and in multiple copies per site. A rib operon having at least five genes is used to make such recombinant bacteria.

US Patent:

6723916, Apr 20, 2004

Filed:

Feb 18, 2003

Appl. No.:

10/368828

Inventors:

Brian F. Flaherty - Stoneham MA
Jonathan E. Mitchel - Norwood MA
John M. Perkins - Wilmington MA
Ronald P. Romano - Acton MA

Assignee:

Parker-Hannifin Corporation - Cleveland OH

International Classification:

H05K 900

US Classification:

174 35GC, 277920

Abstract:

A combination EMI shielding and environmental sealing gasket construction and method for its manufacture. The gasket includes at least one electrically-conductive EMI shielding gasket member, and at least one environmental sealing gasket member. The shielding gasket member, which may be in the form of a strip, has a lengthwise extent and an outer periphery having interstitial spaces, such as between the fibers of a metal wire or other fiber mesh or fabric, through a widthwise extent thereof. The sealing gasket member extends along the lengthwise extent of the shielding gasket member and is formed, such as by being dispensed to foam or otherwise formed-in-place along an adjacent side of the strip, of an elastomeric foam or other material which penetrates into at least a portion of the interstitial spaces of the shielding gasket member to bond the shielding gasket member and the sealing gasket member into an integral structure.

US Patent:

6737256, May 18, 2004

Filed:

Jul 14, 1997

Appl. No.:

08/914332

Inventors:

Scott W. Van Arsdell - Lexington MA
R. Rogers Yocum - Lexington MA
John B. Perkins - Reading MA
Janice G. Pero - Lexington MA

Assignee:

Roche Vitamins Inc. - Parsippany NJ

International Classification:

C12P 1718

US Classification:

435119, 43525231, 435128, 435130, 435193, 435440, 4353201, 536 232

Abstract:

A method is disclosed for the increased production of biotin and the biotin precursor dethiobiotin using a bacterium that produces a lysine-utilizing DAPA aminotransferase. This method involves the use of a bacterium that is either grown in the presence of lysine or deregulated for lysine biosynthesis.

US Patent:

6841366, Jan 11, 2005

Filed:

Sep 28, 1999

Appl. No.:

09/407728

Inventors:

Stanley Grant Bower - Arlington MA, US
John B. Perkins - Reading MA, US
R. Rogers Yocum - Lexington MA, US
Janice G. Pero - Lexington MA, US

Assignee:

DSM IP Assets B.V. - Heerlen

International Classification:

C12N 900

US Classification:

435183, 435189, 435193, 435243, 4352525, 4353201, 536 231

Abstract:

The invention generally provides the genes of the biotin biosynthetic operon of , and closely related species thereof, and well as constructions useful for high level production of biotin.

US Patent:

7091000, Aug 15, 2006

Filed:

Feb 10, 2003

Appl. No.:

10/361522

Inventors:

John B. Perkins - Reading MA, US
Alan Sloma - Watertown MA, US
Janice G. Pero - Lexington MA, US
Randolph T. Hatch - Wellesley MA, US
Theron Hermann - Framingham MA, US
Thomas Erdenberger - Arlington MA, US

Assignee:

DSM Nutritional Products, Inc. - Parsippany NJ

International Classification:

C12P 21/00
C07H 21/04
A61K 38/16
A61K 38/43

US Classification:

435 681, 435 691, 435 711, 514 12, 536 232, 536 237

Abstract:

Vectors and recombinant bacteria for overproducing riboflavin, in which nucleic acid overproducing riboflavin biosynthetic proteins is introduced in the chromosome of the host organism, e. g. at multiple sites and in multiple copies per site. A rib operon having at least five genes is used to make such recombinant bacteria.

US Patent:

7435571, Oct 14, 2008

Filed:

Feb 13, 2004

Appl. No.:

10/546109

Inventors:

Markus Huembelin - Basel, CH
Rual Lopez-Ulibarri - Sisseln, CH
John B. Perkins - Reading MA, US
Ghislain Schyns - Aesch, CH

Assignee:

DSM IP Assets B.V. - Heerlen

International Classification:

C12N 9/12
C12N 9/10
C12N 15/00
C12N 1/20
C07H 21/04

US Classification:

435193, 435194, 4353201, 4352523, 536 232

Abstract:

The present Invention relates to enzymes involved in the Synthesis of Coenzyme Q-10, i. e. , decaprenyl diphosphate (DPP) synthase and 4-hydroxybenzoate polyprenyltransferase, to isolated DNA encoding said enzymes and to methods for the microbial production of Coenzyme Q-10.

US Patent:

20040146997, Jul 29, 2004

Filed:

Jan 9, 2004

Appl. No.:

10/754982

Inventors:

Scott Van Arsdell - Lexington MA, US
R. Rogers Yocum - Lexington MA, US
John Perkins - Reading MA, US
Janice Pero - Lexington MA, US

Assignee:

ROCHE VITAMINS, INC.

International Classification:

C12P017/18
C12N001/21
C12N009/10

US Classification:

435/119000, 435/252300, 435/193000

Abstract:

A method is disclosed for the increased production of biotin and the biotin precursor dethiobiotin using a bacterium that produces a lysine-utilizing DAPA aminotransferase. The method involves the use of a bacterium that is either grown in the presence of lysine or deregulated for lysine biosynthesis.

US Patent:

20070196903, Aug 23, 2007

Filed:

Apr 11, 2007

Appl. No.:

11/786542

Inventors:

Scott Van Arsdell - Lexington MA, US
R. Yocum - Lexington MA, US
John Perkins - Reading MA, US
Janice Pero - Lexington MA, US

International Classification:

C12P 17/18
C12N 9/10
C12N 1/21

US Classification:

435119000, 435252300, 435193000

Abstract:

A method is disclosed for the increased production of biotin and the biotin precursor dethiobiotin using a bacterium that produces a lysine-utilizing DAPA aminotransferase. The method involves the use of a bacterium that is either grown in the presence of lysine or deregulated for lysine biosynthesis.