John James Regan

US Patent:

8298763, Oct 30, 2012

Filed:

Feb 27, 2008

Appl. No.:

12/038109

Inventors:

John Frederick Regan - San Mateo CA, US

Assignee:

Lawrence Livermore National Security, LLC - Livermore CA

International Classification:

C12Q 1/68
B01L 3/00
B01L 99/00
B01D 21/00

US Classification:

435 61, 4352831, 422 681, 422501, 422527, 422537, 422539, 536 231

Abstract:

An automated real-time flow-through system capable of processing multiple samples in an asynchronous, simultaneous, and parallel fashion for nucleic acid extraction and purification, followed by assay assembly, genetic amplification, multiplex detection, analysis, and decontamination. The system is able to hold and access an unlimited number of fluorescent reagents that may be used to screen samples for the presence of specific sequences. The apparatus works by associating extracted and purified sample with a series of reagent plugs that have been formed in a flow channel and delivered to a flow-through real-time amplification detector that has a multiplicity of optical windows, to which the sample-reagent plugs are placed in an operative position. The diagnostic apparatus includes sample multi-position valves, a master sample multi-position valve, a master reagent multi-position valve, reagent multi-position valves, and an optical amplification/detection system.

US Patent:

8647573, Feb 11, 2014

Filed:

Feb 25, 2010

Appl. No.:

12/712345

Inventors:

John Frederick Regan - San Mateo CA, US
James Michael Birch - Albany CA, US

Assignee:

Lawrence Livermore National Security, LLC - Livermore CA

International Classification:

G01N 27/00

US Classification:

422 63

Abstract:

An automated and autonomous diagnostic apparatus that is capable of dispensing collection vials and collections kits to users interesting in collecting a biological sample and submitting their collected sample contained within a collection vial into the apparatus for automated diagnostic services. The user communicates with the apparatus through a touch-screen monitor. A user is able to enter personnel information into the apparatus including medical history, insurance information, co-payment, and answer a series of questions regarding their illness, which is used to determine the assay most likely to yield a positive result. Remotely-located physicians can communicate with users of the apparatus using video tele-medicine and request specific assays to be performed. The apparatus archives submitted samples for additional testing. Users may receive their assay results electronically.

US Patent:

20080213872, Sep 4, 2008

Filed:

Feb 28, 2008

Appl. No.:

12/038981

Inventors:

John Frederick Regan - San Mateo CA, US

International Classification:

C12M 1/12
B01L 9/00
C07H 21/04
B01D 35/00

US Classification:

4352831, 422255, 422104, 536 2541

Abstract:

Removable cartridges are used on automated flow-through systems for the purpose of extracting and purifying genetic material from complex matrices. Different types of cartridges are paired with specific automated protocols to concentrate, extract, and purifying pathogenic or human genetic material. Their flow-through nature allows large quantities sample to be processed. Matrices may be filtered using size exclusion and/or affinity filters to concentrate the pathogen of interest. Lysed material is ultimately passed through a filter to remove the insoluble material before the soluble genetic material is delivered past a silica-like membrane that binds the genetic material, where it is washed, dried, and eluted. Cartridges are inserted into the housing areas of flow-through automated instruments, which are equipped with sensors to ensure proper placement and usage of the cartridges. Properly inserted cartridges create fluid- and air-tight seals with the flow lines of an automated instrument.

US Patent:

20090137047, May 28, 2009

Filed:

Feb 28, 2008

Appl. No.:

12/039069

Inventors:

John Frederick Regan - San Mateo CA, US
James Michael Birch - Albany CA, US

International Classification:

G01N 35/10

US Classification:

436 48, 422 67

Abstract:

An automated and autonomous diagnostic apparatus that is capable of dispensing collection vials and collections kits to users interesting in collecting a biological sample and submitting their collected sample contained within a collection vial into the apparatus for automated diagnostic services. The user communicates with the apparatus through a touch-screen monitor. A user is able to enter personnel information into the apparatus including medical history, insurance information, co-payment, and answer a series of questions regarding their illness, which is used to determine the assay most likely to yield a positive result. Remotely-located physicians can communicate with users of the apparatus using video tele-medicine and request specific assays to be performed. The apparatus archives submitted samples for additional testing. Users may receive their assay results electronically. Users may allow the uploading of their diagnoses into a central databank for disease surveillance purposes.

US Patent:

20120241703, Sep 27, 2012

Filed:

Mar 25, 2011

Appl. No.:

13/071811

Inventors:

John E. Regan - Walnut Creek CA, US

International Classification:

F16M 13/00
E04H 17/20

US Classification:

256 6503, 248539, 248514

Abstract:

A bracket system for connecting a structural member to an upright member includes a bracket having an attachment portion configured to be operatively attached to a vertical or side surface of the structural member and a cup portion integral with the attachment portion. The cup portion includes a base portion and an interior perimeter surface configured to receive the upright member in an installed position such that the exterior perimeter surface of the upright member is proximate to the interior perimeter surface of the cup portion and the upright member is supported in an orientation which is substantially perpendicular and parallel to the structural member. The bracket may include an adjustable member received through a bracket opening and adjustable to retain the upright member in or allow removal of the upright member from the bracket. The upright member may include a reinforcing face plate interfacing with the adjustable member.

US Patent:

20120322058, Dec 20, 2012

Filed:

Feb 9, 2012

Appl. No.:

13/385277

Inventors:

John Frederick Regan - San Mateo CA, US
Serge Saxonov - San Mateo CA, US
Mike Lucero - South San Francisco CA, US
Ben Hindson - Livermore CA, US
Phil Belgrader - Severna Park MD, US
Simant Dube - Berkeley CA, US
Austin So - Pleasanton CA, US
Jeffrey Clark Mellen - San Francisco CA, US
Nicholas Jack Heredia - Mountain House CA, US
Kevin Ness - San Mateo CA, US
Bill Colston - San Ramon CA, US

Assignee:

Bio-Rad Laboratories - Hercules CA

International Classification:

C12Q 1/68

US Classification:

435 611, 435 61, 435 612

Abstract:

Provided herein are improved methods, compositions, and kits for analysis of nucleic acids. The improved methods, compositions, and kits can enable copy number estimation of a nucleic acid in a sample. Also provided herein are methods, compositions, and kits for determining the linkage of two or more copies of a target nucleic acid in a sample (e.g., whether the two or more copies are on the same chromosome or different chromosomes) or for phasing alleles.

US Patent:

20130005587, Jan 3, 2013

Filed:

Sep 7, 2012

Appl. No.:

13/607494

Inventors:

John Frederick Regan - San Mateo CA, US

Assignee:

Lawrence Livermore National Security, LLC - Livermore CA

International Classification:

C40B 20/00

US Classification:

506 2

Abstract:

An automated real-time flow-through system capable of processing multiple samples in an asynchronous, simultaneous, and parallel fashion for nucleic acid extraction and purification, followed by assay assembly, genetic amplification, multiplex detection, analysis, and decontamination. The system is able to hold and access an unlimited number of fluorescent reagents that may be used to screen samples for the presence of specific sequences. The apparatus works by associating extracted and purified sample with a series of reagent plugs that have been formed in a flow channel and delivered to a flow-through real-time amplification detector that has a multiplicity of optical windows, to which the sample-reagent plugs are placed in an operative position. The diagnostic apparatus includes sample multi-position valves, a master sample multi-position valve, a master reagent multi-position valve, reagent multi-position valves, and an optical amplification/detection system.

US Patent:

20130040841, Feb 14, 2013

Filed:

Jul 12, 2012

Appl. No.:

13/548062

Inventors:

Serge Saxonov - Oakland CA, US
Simant Dube - Pleasanton CA, US
John Frederick Regan - San Mateo CA, US

Assignee:

Bio-Rad Laboratories, Inc. - Hercules CA

International Classification:

C40B 30/04

US Classification:

506 9

Abstract:

System, including methods and apparatus, for performing a digital assay with multiplexed detection of two or more distinct targets in the same optical channel.