John R Richards

US Patent:

20020164591, Nov 7, 2002

Filed:

Feb 27, 2001

Appl. No.:

09/794262

Inventors:

John Brandis - Hercules CA, US
Curtis Bloom - Chino Hills CA, US
John Richards - Bradbury CA, US

Assignee:

The Perkin-Elmer Corporation

International Classification:

C12Q001/68
C12P019/34
C12N009/22
C12N009/12
C12P021/02
C12N005/06

US Classification:

435/006000, 435/091200, 435/199000, 435/069100, 435/325000, 435/320100

Abstract:

The present invention relates to mutant DNA polymerases that exhibit reduced discrimination against labeled nucleotides into polynucleotides. The DNA polymerases of the invention have at least one mutation in the nucleotide label interaction region of the enzyme such the mutation results in reduced discrimination against labeled nucleotides. The nucleotide label interaction regions is located at portions of the O-helix, (ii) the K helix, and (iii) the inter O—P helical loop of Taq DNA polymerase or analogous positions in other DNA polymerases. In addition to providing novel mutant DNA polymerases, the invention also provides polynucleotides encoding the subject mutant DNA polymerases. The polynucleotides provided may comprise expression vectors for the recombinant production of the mutant polymerases. The invention also provide host cells containing the subject polynucleotides. The invention also includes numerous methods of using the subject DNA polymerases, including uses for chain termination sequencing and PCR. Another aspect of the invention is to provide kits for synthesizing fluorescently labeled polynucleotides in accordance with the methods of the invention. Kits of the invention comprise a mutant DNA polymerase of the invention and a fluorescently labeled nucleotide that exhibits reduced discrimination with respect to the mutant DNA polymerase in the kit.

US Patent:

6232067, May 15, 2001

Filed:

Aug 17, 1998

Appl. No.:

9/135381

Inventors:

Michael W. Hunkapiller - San Carlos CA
John H. Richards - Bradbury CA

Assignee:

The Perkin-Elmer Corporation - Foster City CA

International Classification:

C12Q 168

US Classification:

435 6

Abstract:

The present invention relate to methods and compositions for simultaneously analyzing multiple different polynucleotides of a polynucleotide composition comprising multiple diverse polynucleotide sequences. The subject methods and compositions may also be applied to analyze or identify single polynucleotides; however, the subject methods and compositions are particularly useful for analyzing large diverse populations of polynucleotides, e. g. , cDNA libraries. Most embodiments of the invention involve hybridizing terminus probes (of known base sequence) and internal fragment probes (of known base sequence) at adjacent positions on an adapter-modified restriction fragment generated from polynucleotide for analysis, and subsequently joining the terminus probes and internal fragment probes to each other. The terminus probe hybridizes to bases of restriction endonuclease recognition site present at the terminus of a restriction fragment generated from the polynucleotide for analysis. Internal fragment probes hybridizes to the same strand of the restriction fragment that the terminus probe hybridizes to and hybridizes to the restriction fragment portion of adapter-modified representative restriction fragments.

US Patent:

62651935, Jul 24, 2001

Filed:

Mar 12, 1998

Appl. No.:

9/041878

Inventors:

John Brandis - Hercules CA
Curtis Bloom - Chino Hills CA
John H. Richards - Bradbury CA

Assignee:

PE Corporation (NY) - Foster City CA
California Institute of Technology - Pasadena CA

International Classification:

C12N 912

US Classification:

435194

Abstract:

The present invention relates to mutant DNA polymerases that exhibit reduced discrimination against labeled nucleotides into polynucleotides. The DNA polymerases of the invention have at least one mutation in the nucleotide label interaction region of the enzyme such the mutation results in reduced discrimination against labeled nucleotides. The nucleotide label interaction regions is located at portions of the O-helix, (ii) the K helix, and (iii) the inter O-P helical loop of Taq DNA polymerase or analogous positions in other DNA polymerases. In addition to providing novel mutant DNA polymerases, the invention also provides polynucleotides encoding the subject mutant DNA polymerases. The polynucleotides provided may comprise expression vectors for the recombinant production of the mutant polymerases. The invention also provide host cells containing the subject polynucleotides.

US Patent:

59124270, Jun 15, 1999

Filed:

Jan 31, 1995

Appl. No.:

8/381170

Inventors:

Kenneth Ellsworth Willis - Redwood City CA
Martin Gerald Richman - Salinas CA
William David Fahey - Cupertino CA
John Gareth Richards - San Jose CA
David S. Whang - San Jose CA

Assignee:

Quantic Industries, Inc. - San Carlos CA

International Classification:

F42B 313

US Classification:

1022028

Abstract:

This invention discloses a method of fabricating an electroexplosive device which utilizes a semiconductor bridge as an ignition element. The semiconductor bridge is electrically connected to a metal header by a small, low resistance contact to the extension of bridge material and through an insulating silicon substrate to an eutectic bond created by gold plating on the metal header and the silicon. The second electrode of the bridge circuit is connected via wire bonds to one or two conducting pins which penetrate the metal header and are insulated by surrounding glass. A redundant connection via two conducting pins insulated from the header to one electrode of the semiconductor bridge allows a post assembly test of the integrity of the wire bonds, thereby increasing reliability of the device.

US Patent:

62585398, Jul 10, 2001

Filed:

Apr 30, 1999

Appl. No.:

9/303774

Inventors:

Michael W. Hunkapiller - San Carlos CA
John H. Richards - Bradbury CA

Assignee:

The Perkin-Elmer Corporation - Foster City CA

International Classification:

C12Q 168

US Classification:

435 6

Abstract:

The present invention relate to methods and compositions for simultaneously analyzing multiple different polynucleotides of a polynucleotide composition comprising multiple diverse polynucleotide sequences. The subject methods and compositions may also be applied to analyze or identify single polynucleotides; however, the subject methods and compositions are particularly useful for analyzing large diverse populations of polynucleotides, e. g. , cDNA libraries. Most embodiments of the invention involve hybridizing terminus probes (of known base sequence) to adapter-modified restriction fragment generated from polynucleotide for analysis, and subsequently joining the terminus probes and internal fragment probes to each other. The terminus probe hybridizes to bases of restriction endonuclease recognition site present at the terminus of a restriction fragment generated from the polynucleotide for analysis. The terminus probes and internal fragment probes may be marked so as to facilitate the simultaneous testing of multiple polynucleotides for the presence of many possible nucleotide base sequences.