John D Yoder

US Patent:

52253414, Jul 6, 1993

Filed:

Jul 19, 1990

Appl. No.:

7/555271

Inventors:

John I. Yoder - Davis CA
Michael W. Lassner - Davis CA

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C12N 1563
C12N 1582
A01H 400

US Classification:

4351723

Abstract:

This invention relates to methods for producing transgenic plants that contain a gene of interest and that are free of foreign ancillary nucleic acids. These methods allow for the production of plants which thus contain a desired gene, but which are free of vector sequences and/or marker sequences used to transform the plant. The method of transforming such plants calls for transforming the plants with a gene of interest by introduction of the gene on a DNA construct comprising a transposon and foreign ancillary nucleic acids; crossing the transformed plant through self-crossing or with another plant to obtain F. sub. 1 or more removed generation progeny; and utilizing a means for selecting those progeny that carry the gene of interest and are free of the ancillary nucleic acids. Such progeny may be detected biochemically, by Southern hybridization, through the use of polymerase chain reaction procedures and other methods available in the art.