Jon K Magnuson

US Patent:

8304212, Nov 6, 2012

Filed:

Jul 10, 2007

Appl. No.:

11/775777

Inventors:

Marco A. Baez-Vasquez - Jupiter FL, US
Richard Burlingame - Jupiter FL, US
Jon K. Magnuson - Richland WA, US
Marion Bradford - Hendersonville NC, US
Arkady Panteleimonovich Sinitsyn - Moscow, RU

Assignee:

Dyadic International, Inc. - Jupiter FL
Battelle Memorial Institute - Richland WA
Iowa Corn Promotion Board - Johnston IA

International Classification:

C12P 19/00
C12N 9/00
C12N 9/14
C12N 1/00

US Classification:

435 72, 435183, 435195, 435243, 4352541

Abstract:

Methods to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material are provided, as well as novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass.

US Patent:

20060040342, Feb 23, 2006

Filed:

Aug 17, 2004

Appl. No.:

10/920625

Inventors:

Ziyu Dai - Richland WA, US
Linda Lasure - Fall City WA, US
Jon Magnuson - Pasco WA, US

Assignee:

Battelle Memorial Institute - Richland WA

International Classification:

C12P 37/00
C12P 21/06
C12P 17/06
C12P 7/62
C12P 7/46
C12N 1/18
C12N 15/74

US Classification:

435043000, 435069100, 435484000, 435254300, 435145000, 435125000, 435135000

Abstract:

The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.

US Patent:

20080085341, Apr 10, 2008

Filed:

Aug 29, 2007

Appl. No.:

11/846762

Inventors:

Ziyu Dai - Richland WA, US
Linda Lasure - Fall City WA, US
Scott Baker - Richland WA, US
Jon Magnuson - Kennewick WA, US

International Classification:

C12P 7/14
C12N 1/00
C12N 1/20
C13K 1/06
C12P 19/00

US Classification:

426048000, 435162000, 435252300, 435255100, 435072000

Abstract:

Methods and microorganisms for forming fermentation products utilizing a microorganism having at least one heterologous gene sequence that enables carbohydrate conversion and carbon dioxide fixation in the production of the fermentation products are disclosed according to some aspects.

US Patent:

20090068723, Mar 12, 2009

Filed:

Oct 23, 2008

Appl. No.:

12/257261

Inventors:

Ziyu Dai - Richland WA, US
Linda L. Lasure - Fall City WA, US
Jon K. Magnuson - Pasco WA, US

Assignee:

BATTELLE MEMORIAL INSTITUTE - Richland WA

International Classification:

C12N 1/21
C12N 15/11
C12N 15/00

US Classification:

43525233, 536 241, 4353201, 4352523

Abstract:

The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.

US Patent:

20210388399, Dec 16, 2021

Filed:

Jun 14, 2021

Appl. No.:

17/347109

Inventors:

- Richland WA, US
Jon K. Magnuson - Richland WA, US
Joonhoon Kim - Berkeley CA, US
Kyle R. Pomraning - Richland WA, US
Ziyu Dai - Richland WA, US
Beth A. Hofstad - Richland WA, US

Assignee:

Battelle Memorial Institute - Richland WA

International Classification:

C12P 7/46
C12N 1/14

Abstract:

Recombinant genetically modified to increase expression of g8846, renamed herein as aconitic acid exporter (aexA), are provided, which in some examples are also genetically inactivated for an endogenous cis-aconitic acid decarboxylase (cadA) gene. Such recombinant produce more aconitic acid as compared to native . Also provided are methods of using such recombinant to increase production of aconitic acid and other organic acids, such as citric acid, itaconic acid, and 3-hydroxypropionic acid (3-HP).

US Patent:

20210163966, Jun 3, 2021

Filed:

Feb 10, 2021

Appl. No.:

17/172660

Inventors:

- Richland WA, US
Ziyu Dai - Richland WA, US
Jon K. Magnuson - Richland WA, US

Assignee:

Battelle Memorial Institute - Richland WA

International Classification:

C12N 15/80
C12N 9/88
C12N 9/10
C12P 7/42
C12N 9/04
C12P 7/48

Abstract:

This application provides recombinant fungi having an endogenous cis-aconitic acid decarboxylase (cadA) gene genetically inactivated, which allows aconitic acid production by the recombinant fungi. Such recombinant fungi can further include an exogenous nucleic acid molecule encoding aspartate decarboxylase (panD), an exogenous nucleic acid molecule encoding β-alanine-pyruvate aminotransferase (BAPAT), and an exogenous nucleic acid molecule encoding 3-hydroxypropironate dehydrogenase (HPDH). Kits including these fungi, and methods of using these fungi to produce aconitic acid and 3-hydroxypropionic acid (3-HP) are also provided.

US Patent:

20190323020, Oct 24, 2019

Filed:

Apr 24, 2019

Appl. No.:

16/393149

Inventors:

- Richland WA, US
Ziyu Dai - Richland WA, US
Jon K. Magnuson - Richland WA, US

Assignee:

Battelle Memorial Institute - Richland WA

International Classification:

C12N 15/80
C12N 9/88
C12N 9/10
C12N 9/04
C12P 7/48
C12P 7/42

Abstract:

This application provides recombinant fungi having an endogenous cis-aconitic acid decarboxylase (cadA) gene genetically inactivated, which allows aconitic acid production by the recombinant fungi. Such recombinant fungi can further include an exogenous nucleic acid molecule encoding aspartate decarboxylase (panD), an exogenous nucleic acid molecule encoding β-alanine-pyruvate aminotransferase (BAPAT), and an exogenous nucleic acid molecule encoding 3-hydroxypropironate dehydrogenase (HPDH). Kits including these fungi, and methods of using these fungi to produce aconitic acid and 3-hydroxypropionic acid (3-HP) are also provided.

US Patent:

20190169584, Jun 6, 2019

Filed:

Sep 6, 2018

Appl. No.:

16/124127

Inventors:

- Oakland CA, US
- Albuquerque NM, US
- Richland WA, US
Jon K. Magnuson - Richland WA, US
Scott E. Baker - Richland WA, US
Blake A. Simmons - San Francisco CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C12N 9/30
C12N 15/80

Abstract:

The present invention provides for an host cell comprising a gene of interest operatively linked to an ecm33 promoter of an ascomycete fungi, wherein the gene of interest is heterologous to the ecm33 promoter and/or to . In some embodiments, the gene of interest is a glycoside hydrolase enzyme. In some embodiments, the glycoside hydrolase enzyme is a glucosidase.