Jonathan D Chen

US Patent:

20080075680, Mar 27, 2008

Filed:

Sep 12, 2007

Appl. No.:

11/898512

Inventors:

Jonathan Y. Chen - Gwynn Oak MD, US

International Classification:

A61K 8/00
A61P 43/00
C12N 1/02

US Classification:

424 701, 435261

Abstract:

The culture of microorganisms endogenous to plants is a process for increasing the number of such microorganisms and collecting the enriched culture media for nutritional, pharmaceutical and cosmetic use. The process includes the steps of: (a) separating plant components (e.g., leaves, fruit, etc.) into smaller parts; (b) immersing the smaller plant parts in a culture medium of an aqueous solution of sodium chloride and sucrose; (c) permitting the immersed parts to stand in the culture medium for between about 7-14 days; (d) removing the smaller plant parts from the culture medium; (e) dividing the culture medium into two portions; (f) diluting each of the portions 1:2 with water; (g) permitting the diluted portions to stand between about 7-14 days to ferment; (h) filtering the diluted portions; and (i) collecting the supernatant. Steps (e) through (g) may be repeated as often as desired. The process may also include maintaining the concentration of sucrose.