Jonathan H Edwards

US Patent:

20120309037, Dec 6, 2012

Filed:

Dec 8, 2010

Appl. No.:

13/514744

Inventors:

Matthew Robert Redinbo - Chapel Hill NC, US
Andrew Clinton Hemmert - Murray UT, US
Jonathan Seaver Edwards - Boston MA, US

International Classification:

C12Q 1/44
G01N 27/00
C12N 11/14
C12Q 1/46
G01N 21/78
G01N 21/75
C12M 1/40
B82Y 15/00

US Classification:

435 20, 435 19, 4352871, 4352887, 435176, 977773, 977902

Abstract:

The present invention provides methods and devices for detecting and identifying toxins, including but not limited to organophosphorus nerve agents and/or organophosphorus pesticides, in a sample. One embodiment of the present invention comprises a method for identifying an organophosphorus nerve agent and/or an organophosphorus pesticide, comprising: exposing a group of enzymes comprising human carboxylesterase 1, at least one mutant of human carboxylesterase 1, and acetylcholinesterase to a sample, wherein the enzymes are separate from each other and each enzyme binds at least one organophosphorus nerve agent or at least one organophosphorus pesticide; contacting the exposed enzymes with a fluid comprising an oxime and a substrate; and detecting a signal produced upon reaction of the substrate and the exposed enzymes, whereby detection of the signal identifies the organophosphorus nerve agent and/or the organophosphorus pesticide.