Jonathan E Ellis

US Patent:

20200072834, Mar 5, 2020

Filed:

Aug 30, 2019

Appl. No.:

16/556632

Inventors:

William Busa - Rancho Cordova CA, US
Philip H. Coelho - Rancho Cordova CA, US
Jonathan Ellis - Rancho Cordova CA, US
Dalip Sethi - Rancho Cordova CA, US

International Classification:

G01N 33/569
B01D 21/26

Abstract:

Disclosed herein are methods for isolating target cells from blood, involving mixing in an open container an undiluted blood sample having a volume of 10 ml or less, and binding agents, wherein each binding agent comprises (A) a primary binding agent comprising an agent capable of binding to at least one cellular epitope on target cells in the undiluted blood sample, (B) a first linker bound to the primary binding agent, to generate binding agent-attached target cells in the undiluted blood sample; contacting the binding agent-attached target cells in the undiluted blood sample with a plurality of buoyant reagents that include a second linker capable of binding to the first linker to generate an undiluted buoyant reagent-attached target cell mixture; diluting the undiluted buoyant reagent-attached target cell mixture by at least 20% to produce a diluted buoyant reagent-attached target cell mixture; applying a vectorial force, such as centrifugal force, to the diluted buoyant reagent-attached target cell mixture to generate a stratified diluted buoyant reagent-attached target cell mixture; removing the buoyant reagent-attached target cells from the stratified diluted buoyant reagent-attached target cell mixture; and isolating the target cells from the buoyant reagent-attached target cells.

US Patent:

20190282619, Sep 19, 2019

Filed:

Mar 14, 2019

Appl. No.:

16/353071

Inventors:

- Rancho Cordova CA, US
William Busa - Rancho Cordova CA, US
Jonathan Ellis - Rancho Cordova CA, US
Dalip Sethi - Rancho Cordova CA, US

International Classification:

A61K 35/17
A61K 47/65
C07K 14/725
C07K 16/18
A61K 9/127
A61K 47/69

Abstract:

Disclosed herein are methods for contacting in a closed container a host liquid including target cells, microbubble reagents comprising gas-core lipid-shelled microbubbles, and one or more antibodies or other ligands that bind to cell surface molecules on the target cells, wherein the one or more antibodies or other ligands are bound to the target cells or the microbubbles, wherein the contacting under conditions to produce target cells linked to microbubbles via the one or more antibodies or other ligands and activating the target cells to generate activated target cells.

US Patent:

20170349875, Dec 7, 2017

Filed:

Jun 29, 2017

Appl. No.:

15/637573

Inventors:

- Sacramento CA, US
William Busa - Sacramento CA, US
Jonathan Ellis - Sacramento CA, US

International Classification:

C12M 1/00
A61M 1/36
C12M 3/00
G01N 1/34
A61M 1/02
C12M 1/06
B04B 13/00

Abstract:

Disclosed herein are cell separation devices, methods and systems, as well as compositions and reagents for use in cell separation methods.

US Patent:

20170224904, Aug 10, 2017

Filed:

Apr 18, 2017

Appl. No.:

15/489968

Inventors:

- Sacramento CA, US
William Busa - Sacramento CA, US
Jonathan Ellis - Sacramento CA, US

International Classification:

A61M 1/36
C12N 5/078

Abstract:

Disclosed herein are cell separation devices, methods and systems, as well as compositions and reagents for use in cell separation methods.

US Patent:

20170183619, Jun 29, 2017

Filed:

Dec 29, 2016

Appl. No.:

15/393628

Inventors:

- Sacramento CA, US
William Busa - Sacramento CA, US
Jonathan Ellis - Sacramento CA, US

International Classification:

C12M 1/00
C12M 3/00
C12M 1/06

Abstract:

Disclosed herein are cell separation devices, methods and systems, as well as compositions and reagents for use in cell separation methods.