Jonathan E Larson

US Patent:

8528589, Sep 10, 2013

Filed:

Mar 23, 2010

Appl. No.:

12/729462

Inventors:

Benjamin Miller - Littleton MA, US
Brian Hutchison - Medford MA, US
Andrew Wilson - Arlington MA, US
Jonathan Larson - Chelsea MA, US
Qun Zhong - Lexington MA, US
Yevgeny Yurkovetsky - Winchester MA, US
Darren Link - Lexington MA, US
Mark Weary - North Billerica MA, US

Assignee:

Raindance Technologies, Inc. - Lexington MA

International Classification:

F16K 31/36

US Classification:

1374875, 137826, 516 54, 436 53

Abstract:

The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.

US Patent:

8535889, Sep 17, 2013

Filed:

Feb 11, 2011

Appl. No.:

13/026107

Inventors:

Jonathan William Larson - Chelsea MA, US
Qun Zhong - Lexington MA, US
Darren Roy Link - Lexington MA, US

Assignee:

Raindance Technologies, Inc. - Lexington MA

International Classification:

C12P 19/34
C12Q 1/68

US Classification:

435 612, 435 911, 435 912

Abstract:

The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

US Patent:

20040214211, Oct 28, 2004

Filed:

Jan 23, 2004

Appl. No.:

10/763567

Inventors:

Rudolf Gilmanshin - Waltham MA, US
Kevin Phillips - Somerville MA, US
Jonathan Larson - New Ipswich NH, US

Assignee:

U.S. Genomics, Inc. - Woburn MA

International Classification:

C12Q001/68
G01N033/53
G06F019/00
G01N033/48
G01N033/50

US Classification:

435/006000, 435/007100, 702/019000

Abstract:

The invention relates to methods and algorithms for processing polymer analysis data. More specifically, the data is analyzed in order to align, orient and discern individual polymer data from a population data set.

US Patent:

20070042406, Feb 22, 2007

Filed:

Jul 13, 2006

Appl. No.:

11/486446

Inventors:

Gregory Yantz - Cambridge MA, US
Jonathan Larson - New Ipswich NH, US
Rudolf Gilmanshin - Waltham MA, US

Assignee:

U.S. Genomics, Inc. - Woburn MA

International Classification:

C12Q 1/68
C12M 3/00

US Classification:

435006000, 435287200

Abstract:

Microfludic channels are constructed for use in preparing and/or analyzing samples. In one embodiment, a microfluidic channel receives a carrier fluid having both non-targets and targets. The non-targets are moved from the carrier fluid by diffusion and into sheathing fluids also present in the channel before contents of the carrier fluid are analyzed.

US Patent:

20070128083, Jun 7, 2007

Filed:

Jul 11, 2006

Appl. No.:

11/484330

Inventors:

Gregory Yantz - Cambridge MA, US
Jonathan Larson - New Ipswich NH, US

Assignee:

U.S. Genomics, Inc. - Woburn MA

International Classification:

B01L 3/00

US Classification:

422100000

Abstract:

Microfludic channels are constructed for use in preparing and/or analyzing samples. In one embodiment, a microchannel delivers multiple fluid samples from an upstream portion to a downstream portion of the channel where each of the fluid samples are separated from one another by a sheathing fluid that also travels toward the downstream portion. In another embodiment, a microchannel is constructed to create multiple elongational flows in series that can align polymers from a coiled state.

US Patent:

20100294665, Nov 25, 2010

Filed:

Jul 11, 2008

Appl. No.:

12/668579

Inventors:

Richard Allen - San Carlos CA, US
Randall E. Burton - Billerica MA, US
Bryan Crane - San Diego CA, US
Jeffrey R. Krogmeier - Stoneham MA, US
Jonathan W. Larson - Chelsea MA, US
Vyacheslav Papkov - Waltham MA, US
Nicaulas Sabourin - Ottawa, CA
Qun Zhong - Lexington MA, US
Yi Zhou - Carlisle MA, US

International Classification:

G01N 27/447

US Classification:

204520, 204627

Abstract:

A system for transferring and/or concentrating a sample is provided. The system includes a chamber with a membrane brave positioned within the chamber. An electrode assembly is configured to create an electric field across the membrane to move a charged sample through the chamber such that the sample collects and may concentrate on the membrane. The system may include a plurality of membranes. The system may also include a plurality of microchannels outwardly extending from the channel, where the membrane extends along the plurality of microchannels. Aspects of the invention are also directed to a pipette which may be used to transfer and concentrate a sample on a membrane. Certain embodiments are directed to methods and systems for concentrating a nucleic acid sample.

US Patent:

20110250597, Oct 13, 2011

Filed:

Feb 11, 2011

Appl. No.:

13/026120

Inventors:

Jonathan William Larson - Chelsea MA, US
Qun Zhong - Lexington MA, US
Darren Roy Link - Lexington MA, US

Assignee:

RAINDANCE TECHNOLOGIES, INC. - Lexington MA

International Classification:

C12Q 1/68

US Classification:

435 611, 435 61, 435 612

Abstract:

The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

US Patent:

20120219947, Aug 30, 2012

Filed:

Feb 10, 2012

Appl. No.:

13/371222

Inventors:

Yevgeny Yurkovetsky - Winchester MA, US
Darren Roy Link - Lexington MA, US
Jonathan William Larson - Chelsea MA, US

Assignee:

RAINDANCE TECHNOLOGIES, INC. - Lexington MA

International Classification:

C12Q 1/68

US Classification:

435 611

Abstract:

The invention generally relates to methods for forming mixed droplets. In certain embodiments, methods of the invention involve forming a droplet, and contacting the droplet with a fluid stream, wherein a portion of the fluid stream integrates with the droplet to form a mixed droplet.