Jonathan A Larson

US Patent:

8535889, Sep 17, 2013

Filed:

Feb 11, 2011

Appl. No.:

13/026107

Inventors:

Jonathan William Larson - Chelsea MA, US
Qun Zhong - Lexington MA, US
Darren Roy Link - Lexington MA, US

Assignee:

Raindance Technologies, Inc. - Lexington MA

International Classification:

C12P 19/34
C12Q 1/68

US Classification:

435 612, 435 911, 435 912

Abstract:

The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

US Patent:

20070042406, Feb 22, 2007

Filed:

Jul 13, 2006

Appl. No.:

11/486446

Inventors:

Gregory Yantz - Cambridge MA, US
Jonathan Larson - New Ipswich NH, US
Rudolf Gilmanshin - Waltham MA, US

Assignee:

U.S. Genomics, Inc. - Woburn MA

International Classification:

C12Q 1/68
C12M 3/00

US Classification:

435006000, 435287200

Abstract:

Microfludic channels are constructed for use in preparing and/or analyzing samples. In one embodiment, a microfluidic channel receives a carrier fluid having both non-targets and targets. The non-targets are moved from the carrier fluid by diffusion and into sheathing fluids also present in the channel before contents of the carrier fluid are analyzed.

US Patent:

20070128083, Jun 7, 2007

Filed:

Jul 11, 2006

Appl. No.:

11/484330

Inventors:

Gregory Yantz - Cambridge MA, US
Jonathan Larson - New Ipswich NH, US

Assignee:

U.S. Genomics, Inc. - Woburn MA

International Classification:

B01L 3/00

US Classification:

422100000

Abstract:

Microfludic channels are constructed for use in preparing and/or analyzing samples. In one embodiment, a microchannel delivers multiple fluid samples from an upstream portion to a downstream portion of the channel where each of the fluid samples are separated from one another by a sheathing fluid that also travels toward the downstream portion. In another embodiment, a microchannel is constructed to create multiple elongational flows in series that can align polymers from a coiled state.

US Patent:

20110250597, Oct 13, 2011

Filed:

Feb 11, 2011

Appl. No.:

13/026120

Inventors:

Jonathan William Larson - Chelsea MA, US
Qun Zhong - Lexington MA, US
Darren Roy Link - Lexington MA, US

Assignee:

RAINDANCE TECHNOLOGIES, INC. - Lexington MA

International Classification:

C12Q 1/68

US Classification:

435 611, 435 61, 435 612

Abstract:

The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

US Patent:

20130196324, Aug 1, 2013

Filed:

Feb 22, 2013

Appl. No.:

13/773868

Inventors:

Jonathan William Larson - Chelsea MA, US
Qun Zhong - Lexington MA, US
Darren Roy Link - Lexington MA, US

International Classification:

C12Q 1/68

US Classification:

435 611, 435 612

Abstract:

The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

US Patent:

20140076430, Mar 20, 2014

Filed:

Sep 9, 2013

Appl. No.:

14/021481

Inventors:

Brian Hutchison - Medford MA, US
Andrew Wilson - Arlington MA, US
Jonathan Larson - Chelsea MA, US
Qun Zhong - Lexington MA, US
Yevgeny Yurkovetsky - Winchester MA, US
Darren Link - Lexington MA, US
Mark Weary - North Billerica MA, US

Assignee:

RAINDANCE TECHNOLOGIES, INC. - Lexington MA

International Classification:

F17D 3/01

US Classification:

137551

Abstract:

The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.

US Patent:

20200225129, Jul 16, 2020

Filed:

Jan 2, 2020

Appl. No.:

16/733137

Inventors:

- Hercules CA, US
Brian Hutchison - Medford MA, US
Andrew Wilson - Arlington MA, US
Jonathan William Larson - Chelsea MA, US
Qun Zhong - Lexington MA, US
Yevgeny Yurkovetsky - Winchester MA, US
Darren Roy Link - Lexington MA, US
Mark Weary - North Billerica MA, US

International Classification:

G01N 1/38
B01L 3/02
B01L 3/00
B01F 15/00
B01F 13/00
F17D 3/01
G01N 27/447
F16K 99/00

Abstract:

The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.

US Patent:

20190390259, Dec 26, 2019

Filed:

Apr 5, 2017

Appl. No.:

16/091858

Inventors:

- Beverly MA, US
Jonathan W. Larson - Chelsea MA, US

International Classification:

C12Q 1/6827
C12Q 1/6886

Abstract:

Compositions for detecting, diagnosing and prognosing cancer in individuals having or suspected of having cancer are provided. Said compositions are used to enrich a nucleic acid target comprising a locus of genetic variation, e.g., single nucleotide polymorphism (SNPs) and variable mutations, such as small insertions, deletions, and replacements (“indels”) within a sample for ease and improved detection. In addition, kits are provided for measuring levels or the presence of SNPs and indels associated with cancer for detecting, diagnosing and prognosing cancer. Furthermore, methods are provided for detecting, diagnosing and prognosing cancer in individuals having or suspected of having cancer comprising determining the enrichment levels and/or presence or absence of the SNPs and indels in a subject.