Jonathan W Larson

US Patent:

8528589, Sep 10, 2013

Filed:

Mar 23, 2010

Appl. No.:

12/729462

Inventors:

Benjamin Miller - Littleton MA, US
Brian Hutchison - Medford MA, US
Andrew Wilson - Arlington MA, US
Jonathan Larson - Chelsea MA, US
Qun Zhong - Lexington MA, US
Yevgeny Yurkovetsky - Winchester MA, US
Darren Link - Lexington MA, US
Mark Weary - North Billerica MA, US

Assignee:

Raindance Technologies, Inc. - Lexington MA

International Classification:

F16K 31/36

US Classification:

1374875, 137826, 516 54, 436 53

Abstract:

The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.

US Patent:

8535889, Sep 17, 2013

Filed:

Feb 11, 2011

Appl. No.:

13/026107

Inventors:

Jonathan William Larson - Chelsea MA, US
Qun Zhong - Lexington MA, US
Darren Roy Link - Lexington MA, US

Assignee:

Raindance Technologies, Inc. - Lexington MA

International Classification:

C12P 19/34
C12Q 1/68

US Classification:

435 612, 435 911, 435 912

Abstract:

The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

US Patent:

7262859, Aug 28, 2007

Filed:

Oct 13, 2005

Appl. No.:

11/250179

Inventors:

Jonathan W. Larson - New Ipswich NH, US
Gregory R. Yantz - Cambridge MA, US
George Seward - Arlington MA, US
David Johnson - Edmonton, CA
Jeffrey R. Krogmeier - Woburn MA, US

Assignee:

U.S. Genomics, Inc. - Woburn MA

International Classification:

G01N 21/55
G01N 21/01

US Classification:

356445, 356244

Abstract:

The invention relates to methods and systems for detecting and or analyzing an agent in a sample with a chip having optical components incorporated therein.

US Patent:

20040214211, Oct 28, 2004

Filed:

Jan 23, 2004

Appl. No.:

10/763567

Inventors:

Rudolf Gilmanshin - Waltham MA, US
Kevin Phillips - Somerville MA, US
Jonathan Larson - New Ipswich NH, US

Assignee:

U.S. Genomics, Inc. - Woburn MA

International Classification:

C12Q001/68
G01N033/53
G06F019/00
G01N033/48
G01N033/50

US Classification:

435/006000, 435/007100, 702/019000

Abstract:

The invention relates to methods and algorithms for processing polymer analysis data. More specifically, the data is analyzed in order to align, orient and discern individual polymer data from a population data set.

US Patent:

20050112606, May 26, 2005

Filed:

Apr 9, 2004

Appl. No.:

10/821664

Inventors:

Martin Fuchs - Uxbridge MA, US
Jonathan Larson - New Ipswich NH, US

International Classification:

C12Q001/68
C12M001/34

US Classification:

435006000, 435287200

Abstract:

A microfluidic device for altering the flow of a carrier fluid containing a polymer, such that, the polymer can be positioned, aligned, or elongated. The device accomplishes these effects by directing the carrier fluid in a laminar flow into obstacles, or other fluids to knowingly alter the path of the carrier fluid streamlines. These streamlines, in turn, apply fluidic drag forces against the polymer to manipulate it into a desired configuration. Other aspects of the device retain a polymer in an aligned or elongated state with crimps which prevent portions of the polymer from coiling. These structures utilize the natural concept of increasing entropy to allow small portions of an aligned or elongated polymer to return to a high entropy, or coiled state, while retaining the majority of the polymer in a low entropy, aligned or elongated state for subsequent analysis or manipulation.

US Patent:

20060134679, Jun 22, 2006

Filed:

Dec 19, 2005

Appl. No.:

11/311696

Inventors:

Jonathan Larson - New Ipswich NH, US

Assignee:

U.S. Genomics, Inc. - Woburn MA

International Classification:

C12Q 1/68

US Classification:

435006000

Abstract:

The invention relates to the methods for analyzing polymers that are in unstretched conformations. In particular, the polymers such as nucleic acids may be present in hairpin conformations.

US Patent:

20070042406, Feb 22, 2007

Filed:

Jul 13, 2006

Appl. No.:

11/486446

Inventors:

Gregory Yantz - Cambridge MA, US
Jonathan Larson - New Ipswich NH, US
Rudolf Gilmanshin - Waltham MA, US

Assignee:

U.S. Genomics, Inc. - Woburn MA

International Classification:

C12Q 1/68
C12M 3/00

US Classification:

435006000, 435287200

Abstract:

Microfludic channels are constructed for use in preparing and/or analyzing samples. In one embodiment, a microfluidic channel receives a carrier fluid having both non-targets and targets. The non-targets are moved from the carrier fluid by diffusion and into sheathing fluids also present in the channel before contents of the carrier fluid are analyzed.

US Patent:

20070128083, Jun 7, 2007

Filed:

Jul 11, 2006

Appl. No.:

11/484330

Inventors:

Gregory Yantz - Cambridge MA, US
Jonathan Larson - New Ipswich NH, US

Assignee:

U.S. Genomics, Inc. - Woburn MA

International Classification:

B01L 3/00

US Classification:

422100000

Abstract:

Microfludic channels are constructed for use in preparing and/or analyzing samples. In one embodiment, a microchannel delivers multiple fluid samples from an upstream portion to a downstream portion of the channel where each of the fluid samples are separated from one another by a sheathing fluid that also travels toward the downstream portion. In another embodiment, a microchannel is constructed to create multiple elongational flows in series that can align polymers from a coiled state.