Jonathan D Larson

US Patent:

8535889, Sep 17, 2013

Filed:

Feb 11, 2011

Appl. No.:

13/026107

Inventors:

Jonathan William Larson - Chelsea MA, US
Qun Zhong - Lexington MA, US
Darren Roy Link - Lexington MA, US

Assignee:

Raindance Technologies, Inc. - Lexington MA

International Classification:

C12P 19/34
C12Q 1/68

US Classification:

435 612, 435 911, 435 912

Abstract:

The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

US Patent:

20040214211, Oct 28, 2004

Filed:

Jan 23, 2004

Appl. No.:

10/763567

Inventors:

Rudolf Gilmanshin - Waltham MA, US
Kevin Phillips - Somerville MA, US
Jonathan Larson - New Ipswich NH, US

Assignee:

U.S. Genomics, Inc. - Woburn MA

International Classification:

C12Q001/68
G01N033/53
G06F019/00
G01N033/48
G01N033/50

US Classification:

435/006000, 435/007100, 702/019000

Abstract:

The invention relates to methods and algorithms for processing polymer analysis data. More specifically, the data is analyzed in order to align, orient and discern individual polymer data from a population data set.

US Patent:

20070042406, Feb 22, 2007

Filed:

Jul 13, 2006

Appl. No.:

11/486446

Inventors:

Gregory Yantz - Cambridge MA, US
Jonathan Larson - New Ipswich NH, US
Rudolf Gilmanshin - Waltham MA, US

Assignee:

U.S. Genomics, Inc. - Woburn MA

International Classification:

C12Q 1/68
C12M 3/00

US Classification:

435006000, 435287200

Abstract:

Microfludic channels are constructed for use in preparing and/or analyzing samples. In one embodiment, a microfluidic channel receives a carrier fluid having both non-targets and targets. The non-targets are moved from the carrier fluid by diffusion and into sheathing fluids also present in the channel before contents of the carrier fluid are analyzed.

US Patent:

20070128083, Jun 7, 2007

Filed:

Jul 11, 2006

Appl. No.:

11/484330

Inventors:

Gregory Yantz - Cambridge MA, US
Jonathan Larson - New Ipswich NH, US

Assignee:

U.S. Genomics, Inc. - Woburn MA

International Classification:

B01L 3/00

US Classification:

422100000

Abstract:

Microfludic channels are constructed for use in preparing and/or analyzing samples. In one embodiment, a microchannel delivers multiple fluid samples from an upstream portion to a downstream portion of the channel where each of the fluid samples are separated from one another by a sheathing fluid that also travels toward the downstream portion. In another embodiment, a microchannel is constructed to create multiple elongational flows in series that can align polymers from a coiled state.

US Patent:

20110250597, Oct 13, 2011

Filed:

Feb 11, 2011

Appl. No.:

13/026120

Inventors:

Jonathan William Larson - Chelsea MA, US
Qun Zhong - Lexington MA, US
Darren Roy Link - Lexington MA, US

Assignee:

RAINDANCE TECHNOLOGIES, INC. - Lexington MA

International Classification:

C12Q 1/68

US Classification:

435 611, 435 61, 435 612

Abstract:

The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

US Patent:

20120219947, Aug 30, 2012

Filed:

Feb 10, 2012

Appl. No.:

13/371222

Inventors:

Yevgeny Yurkovetsky - Winchester MA, US
Darren Roy Link - Lexington MA, US
Jonathan William Larson - Chelsea MA, US

Assignee:

RAINDANCE TECHNOLOGIES, INC. - Lexington MA

International Classification:

C12Q 1/68

US Classification:

435 611

Abstract:

The invention generally relates to methods for forming mixed droplets. In certain embodiments, methods of the invention involve forming a droplet, and contacting the droplet with a fluid stream, wherein a portion of the fluid stream integrates with the droplet to form a mixed droplet.

US Patent:

20130000738, Jan 3, 2013

Filed:

Feb 28, 2012

Appl. No.:

13/406783

Inventors:

Jeffrey R. Krogmeier - Stoneham MA, US
Robert H. Meltzer - Chelmsford MA, US
Jonathan W. Larson - Chelsea MA, US

Assignee:

PathoGenetix, Inc. - Woburn MA

International Classification:

F17D 3/00

US Classification:

137 3

Abstract:

A method of controlling the concentration of a sample flowing through a microchannel is provided. The method includes flowing a sample carrier fluid from a sample carrier channel and into an upstream portion of a microchannel such that the sample carrier fluid flows from the upstream portion and toward a downstream portion of the microchannel, and flowing a sheathing fluid from a sheathing fluid channel and into the upstream portion of the microchannel such that the sheathing fluid flows from the upstream portion and toward the downstream portion of the microchannel, and generally parallel to the sample carrier fluid. The method further includes detecting a concentration of a sample in the sample carrier fluid in the downstream portion of the microchannel, and adjusting a flow rate of at least one of the sample carrier fluid and the sheathing fluid based upon the detected concentration of the sample.

US Patent:

20130196324, Aug 1, 2013

Filed:

Feb 22, 2013

Appl. No.:

13/773868

Inventors:

Jonathan William Larson - Chelsea MA, US
Qun Zhong - Lexington MA, US
Darren Roy Link - Lexington MA, US

International Classification:

C12Q 1/68

US Classification:

435 611, 435 612

Abstract:

The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.