Jonathan G Lin

US Patent:

20210356381, Nov 18, 2021

Filed:

Jul 27, 2021

Appl. No.:

17/386192

Inventors:

- Franklin Lakes NJ, US
Keegan Owsley - Los Angeles CA, US
Jonathan Lin - Los Angeles CA, US

International Classification:

G01N 15/14
G01N 21/64

Abstract:

In one aspect, a method of sorting cells in a flow cytometry system is disclosed, which includes illuminating a cell with radiation having at least two optical frequencies shifted from one another by a radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and processing the temporal fluorescence data to arrive at a sorting decision regarding the cell without generating an image (i.e., a pixel-by-pixel image) of the cell based on the fluorescence data.. In other words, while the fluorescence data can contain image data that would allow generating a pixel-by-pixel fluorescence intensity map, the method arrives at the sorting decision without generating such a map. In some cases, the sorting decision can be made with a latency less than about 100 microseconds, In some embodiments, the above method of sorting cells can have a sub-cellular resolution, e.g., the sorting decision can be based on characteristics of a component of the cell. In some embodiments in which more than two frequency-shifted optical frequencies are employed, a single radiofrequency shift is employed to separate the optical frequencies while in other such embodiments a plurality of different radiofrequency shifts are employed.

US Patent:

20210288984, Sep 16, 2021

Filed:

Mar 12, 2020

Appl. No.:

16/817503

Inventors:

- El Segundo CA, US
Nicholas Cameron COHEN - Long Beach CA, US
Jonathan LIN - El Segundo CA, US
Douglas Robert WOODWARD - Redondo Beach CA, US
Jacquelyn Christina ANDRADE - Rancho Cucamonga CA, US
Eric John MCDONALD - Newbury Park CA, US
Michael Harvey COLE - Torrance CA, US

International Classification:

H04L 29/06
G06N 20/00
G06N 5/04
H04L 12/26
H04L 29/08

Abstract:

Space system TT&C monitoring includes analyzing network traffic comprising of data packets between a front-end processor (FEP) and a cryptographic unit. A JavaScript Object Notation (JSON) object is created when the network traffic containing a vehicle command is detected. The JSON object is transmitted, by way of a data transport mechanism, to either a cyber defense module or a security information and event management (SIEM) module for further ingestions and visualization. The JSON object is analyzed using machine learning (ML) module or a rule-based intrusion detection system (IDS) module to generate an anomaly score for the SIEM module for further ingestions and visualization.

US Patent:

20200209140, Jul 2, 2020

Filed:

Mar 10, 2020

Appl. No.:

16/814394

Inventors:

- Franklin Lakes NJ, US
Keegan Owsley - Los Angeles CA, US
Jonathan Lin - Los Angeles CA, US

International Classification:

G01N 15/14
G01N 21/64

Abstract:

In one aspect, a method of sorting cells in a flow cytometry system is disclosed, which includes illuminating a cell with radiation having at least two optical frequencies shifted from one another by a radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and processing the temporal fluorescence data to arrive at a sorting decision regarding the cell without generating an image (i.e., a pixel-by-pixel image) of the cell based on the fluorescence data. In other words, while the fluorescence data can contain image data that would allow generating a pixel-by-pixel fluorescence intensity map, the method arrives at the sorting decision without generating such a map. In some cases, the sorting decision can be made with a latency less than about 100 microseconds. In some embodiments, the above method of sorting cells can have a sub-cellular resolution, e.g., the sorting decision can be based on characteristics of a component of the cell. In some embodiments in which more than two frequency-shifted optical frequencies are employed, a single radiofrequency shift is employed to separate the optical frequencies while in other such embodiments a plurality of different radiofrequency shifts are employed.

US Patent:

20190234863, Aug 1, 2019

Filed:

Apr 11, 2019

Appl. No.:

16/382056

Inventors:

- Franklin Lakes NJ, US
Keegan Owsley - Los Angeles CA, US
Jonathan Lin - Los Angeles CA, US

International Classification:

G01N 15/14
G01N 21/64

Abstract:

In one aspect, a method of sorting cells in a flow cytometry system is disclosed, which includes illuminating a cell with radiation having at least two optical frequencies shifted from one another by a radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and processing the temporal fluorescence data to arrive at a sorting decision regarding the cell without generating an image (i.e., a pixel-by-pixel image) of the cell based on the fluorescence data. In some cases, the sorting decision can be made with a latency less than about 100 microseconds. In some embodiments, the above method of sorting cells can have a sub-cellular resolution. In some embodiments, a single radiofrequency shift is employed to separate the optical frequencies while in other such embodiments a plurality of different radiofrequency shifts are employed.

US Patent:

20190204208, Jul 4, 2019

Filed:

Mar 11, 2019

Appl. No.:

16/299001

Inventors:

- LOS ANGELES CA, US
Keegan Owsley - Los Angeles CA, US
Jonathan Lin - Los Angeles CA, US

International Classification:

G01N 15/14
G01N 21/64
G02B 21/06

Abstract:

In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. The fluorescence emission from the sample can be detected and analyzed, e.g., to generate a fluorescence image of the sample. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency. For example, a horizontal extent of the sample can be illuminated by a laser beam at a single excitation frequency. The detected fluorescence radiation can be used to analyze the fluorescence content of the sample, e.g., a cell/particle.

US Patent:

20180348113, Dec 6, 2018

Filed:

Aug 8, 2018

Appl. No.:

16/058734

Inventors:

- LOS ANGELES CA, US
Keegan Owsley - Los Angeles CA, US
Jonathan Lin - Los Angeles CA, US

International Classification:

G01N 15/14
G01N 21/64
G01N 15/10

Abstract:

In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. The fluorescence emission from the sample can be detected and analyzed, e.g., to generate a fluorescence image of the sample. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency. For example, a horizontal extent of the sample can be illuminated by a laser beam at a single excitation frequency. The detected fluorescence radiation can be used to analyze the fluorescence content of the sample, e.g., a cell/particle.

US Patent:

20170328826, Nov 16, 2017

Filed:

May 12, 2017

Appl. No.:

15/593959

Inventors:

- San Jose CA, US
Keegan Owsley - Los Angeles CA, US
Jonathan Lin - Los Angeles CA, US
Matthew Bahr - Diamond Bar CA, US

International Classification:

G01N 15/14
G01N 15/14
G06T 7/00
G06T 7/246
G01N 15/14
G01N 21/64
G01N 15/00
G01N 15/10

Abstract:

In one aspect, a system for performing flow cytometry is disclosed, which comprises a laser for generating laser radiation for illuminating a sample, at least one detector for detecting at least a portion of a radiation emanating from the sample in response to said illumination so as to generate a temporal signal corresponding to said detected radiation, and an analysis module for receiving said temporal signal and performing a statistical analysis of said signal based on a forward model to reconstruct an image of said sample.

US Patent:

20170268981, Sep 21, 2017

Filed:

Mar 17, 2017

Appl. No.:

15/462124

Inventors:

- San Jose CA, US
Keegan Owsley - Los Angeles CA, US
Jonathan Lin - Los Angeles CA, US

International Classification:

G01N 15/14
G01N 21/64

Abstract:

In one aspect, a method of sorting cells in a flow cytometry system is disclosed, which includes illuminating a cell with radiation having at least two optical frequencies shifted from one another by a radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and processing the temporal fluorescence data to arrive at a sorting decision regarding the cell without generating an image (i.e., a pixel-by-pixel image) of the cell based on the fluorescence data. In other words, while the fluorescence data can contain image data that would allow generating a pixel-by-pixel fluorescence intensity map, the method arrives at the sorting decision without generating such a map. In some cases, the sorting decision can be made with a latency less than about 100 microseconds. In some embodiments, the above method of sorting cells can have a sub-cellular resolution, e.g., the sorting decision can be based on characteristics of a component of the cell. In some embodiments in which more than two frequency-shifted optical frequencies are employed, a single radiofrequency shift is employed to separate the optical frequencies while in other such embodiments a plurality of different radiofrequency shifts are employed.