Jose D Cano

US Patent:

7390662, Jun 24, 2008

Filed:

Nov 9, 2005

Appl. No.:

11/270865

Inventors:

John S. Riley - Miami FL, US
Jose Cano - Miami FL, US
Valentin Quesada - Miami FL, US
Maritza Lavernia - Miami FL, US
Mark A. Wells - Davie FL, US
Eileen Landrum - Miami FL, US
Carlos A. Perez - Miami FL, US
Christophe Godefroy - Miramar FL, US

Assignee:

Beckman Coulter, Inc. - Fullerton CA

International Classification:

G01N 31/00
G01N 33/48

US Classification:

436 10, 436 8, 436 17, 436 63, 436150, 436164, 435 2, 356337

Abstract:

The present invention relates to a hematology instrument system that discriminates platelets from red blood cells, debris and other particles within a blood sample. The instrument system uses an optical trigger and collects data at optical sensor locations relative to a flow cell-illuminating laser beam's optical axis. An axial sensor measures axial light loss due to a particle in the flow cell's illumination aperture. In addition, the instrument system can include an RF unit generates electrical parameters, such as DC and RF parameters, within the flow cell. Blood specimens can be prepared with and without a sphering agent.

US Patent:

7661326, Feb 16, 2010

Filed:

Oct 26, 2006

Appl. No.:

11/553107

Inventors:

William W. Li - Miami FL, US
William J. Casey - Boca Raton FL, US
Craig R. Veiner - Miami FL, US
Carlos R. Gonzalez - Pinecrest FL, US
Jose M. Cano - Miami FL, US
Roberto Del Valle - Coral Gables FL, US
Santiago Galvez - Miami FL, US

Assignee:

Beckman Coulter, Inc. - Fullerton CA

International Classification:

G01N 1/00

US Classification:

7386373

Abstract:

Apparatus for aspirating and dispensing liquid samples in an analytical instrument, e. g. , a hematology instrument, includes a liquid-sampling valve that, while operating to segment and position for dispensing one or more precise volumes of a liquid sample that has been aspirated into the valve by a pump, simultaneously enables the apparatus to be operated in an aspirate/dispense (suck-and-spit) mode in which a liquid sample can be selectively driven through the valve in opposite directions by a pump, e. g. , a syringe pump.

US Patent:

8094299, Jan 10, 2012

Filed:

Jul 24, 2008

Appl. No.:

12/178817

Inventors:

Mark A. Wells - Davie FL, US
Carlos A. Perez - Miami FL, US
Jose M. Cano - Miami FL, US

Assignee:

Beckman Coulter, Inc. - Brea CA

International Classification:

G01N 21/01

US Classification:

356244

Abstract:

Transducer modules for use in a blood analysis instrument and methods for analyzing a blood sample. The transducer modules presented generally include a light source, a focus-alignment system, a flow cell, and a light scatter detection system. Electrodes within the flow cell allow for the measurement of the DC impedance and RF conductivity of cells passing through a cell-interrogation zone in the flow cell. Light scatter from the cells passing through the cell-interrogation zone is measured by the light scatter detection system. The light scatter detection system measures the light scatter parameters of upper median light scatter, lower median angle light scatter, low angle light scatter, and axial light loss. The presented methods for analyzing a blood sample generally include aspirating a whole blood sample into a blood analysis instrument, preparing the blood sample for analysis, passing the blood sample through a flow cell in a transducer system, and measuring axial light loss, multiple angles of light scatter, DC impedance and/or RF conductivity.

US Patent:

8189187, May 29, 2012

Filed:

Nov 13, 2009

Appl. No.:

12/617897

Inventors:

Marshall Donnie Graham - Nicholasville KY, US
William Gerry Graham - Nicholasville KY, US
James P. Clarkin - Scottsdale AZ, US
Mark A. Wells - Davie FL, US
Jose M. Cano - Miami FL, US
Carlos Alberto Arboleda - Miami FL, US
Armando Jose Sanchez - Miami FL, US

Assignee:

Beckman Coulter, Inc. - Brea CA

International Classification:

G01N 1/10

US Classification:

356246

Abstract:

An improved optical flow cell adapted for use in a flow cytometer for differentiating formed bodies (e. g. , blood cells). Manufactured from a monolithic transparent material, the improved flow cell has an internal flow channel of polygonal transverse cross-section through which prepared samples can be metered and an external envelope suited to acquisition of optical parameters from formed bodies in such samples. Preferably, such flow cell is formed by a glass-drawing process in which a relatively large glass preform having a rectilinear internal channel of a desired polygonal cross-sectional shape is heated and drawn to achieve a desired cross-sectional area of reduced size. Also disclosed are preferred methods for differentiating formed bodies using the flow cell of the invention.

US Patent:

8339585, Dec 25, 2012

Filed:

Dec 6, 2011

Appl. No.:

13/312832

Inventors:

Mark A. Wells - Miami FL, US
Carlos A. Perez - Miami FL, US
Jose M. Cano - Miami FL, US

Assignee:

Beckman Coulter, Inc. - Brea CA

International Classification:

G01N 33/48
G01N 21/00

US Classification:

356 39, 356337, 356343

Abstract:

Transducer modules for use in a blood analysis instrument and methods for analyzing a blood sample. The transducer modules presented generally include a light source, a focus-alignment system, a flow cell, and a light scatter detection system. Electrodes within the flow cell allow for the measurement of the DC impedance and RF conductivity of cells passing through a cell-interrogation zone in the flow cell. Light scatter from the cells passing through the cell-interrogation zone is measured by the light scatter detection system. The light scatter detection system measures the light scatter parameters of upper median light scatter, lower median angle light scatter, low angle light scatter, and axial light loss. The presented methods for analyzing a blood sample generally include aspirating a whole blood sample into a blood analysis instrument, preparing the blood sample for analysis, passing the blood sample through a flow cell in a transducer system, and measuring axial light loss, multiple angles of light scatter, DC impedance and/or RF conductivity.

US Patent:

20100329927, Dec 30, 2010

Filed:

Jun 26, 2009

Appl. No.:

12/492270

Inventors:

Carlos A. Perez - Miami FL, US
Jose M. Cano - Miami FL, US
Joe C. Schorsch - Coconut Grove FL, US
Viviana A. Vargas - Miami FL, US

International Classification:

G01N 33/48
G01N 1/28

US Classification:

422 681, 73 6456

Abstract:

A pipelining assembly for use in a blood analyzing instrument, methods for performing parallel pipelining functions, and methods for processing a plurality of prepared blood samples through a blood analyzing instrument. The pipelining assembly presented generally includes a first sample preparation chamber, a first queuing chamber in fluid communication with the first sample preparation chamber, and a first control valve between the first sample preparation chamber and the first queuing chamber. The pipelining assembly further includes a second sample preparation chamber, a second queuing chamber in fluid communication with the second sample preparation chamber, and a second control valve between the second sample preparation chamber and the second queuing chamber. An analysis chamber is provided to receive first and second prepared blood samples from the in first and second queuing chambers. The presented methods include steps for repeated processing of prepared blood samples through the blood analyzing instrument.

US Patent:

20120208228, Aug 16, 2012

Filed:

Apr 10, 2012

Appl. No.:

13/443581

Inventors:

Carlos A. Perez - Miami FL, US
Jose M. Cano - Miami FL, US
Joe C. Schorsch - Coconut Grove FL, US
Viviana A. Vargas - Miami FL, US

International Classification:

C12Q 1/02

US Classification:

435 29

Abstract:

A pipelining assembly for use in a blood analyzing instrument, methods for performing parallel pipelining functions, and methods for processing a plurality of prepared blood samples through a blood analyzing instrument. The pipelining assembly presented generally includes a first sample preparation chamber, a first queuing chamber in fluid communication with the first sample preparation chamber, and a first control valve between the first sample preparation chamber and the first queuing chamber. The pipelining assembly further includes a second sample preparation chamber, a second queuing chamber in fluid communication with the second sample preparation chamber, and a second control valve between the second sample preparation chamber and the second queuing chamber. An analysis chamber is provided to receive first and second prepared blood samples from the in first and second queuing chambers. The presented methods include steps for repeated processing of prepared blood samples through the blood analyzing instrument.

US Patent:

62286528, May 8, 2001

Filed:

Feb 16, 1999

Appl. No.:

9/251019

Inventors:

Carlos M. Rodriguez - Miami FL
Jose M. Cano - Miami FL
Barbara Carrillo - Miami FL
Kristie M. Gordon - Coral Gables FL
Allan F. Horton - Miami FL
Ronald D. Paul - Fort Lauderdale FL
Mark A. Wells - Davie FL
James L. Wyatt - Plantation FL

Assignee:

Coulter International Corp. - Miami FL

International Classification:

G01N 3348

US Classification:

436 63

Abstract:

A blood analyzing instrument includes a single transducer for simultaneously measuring the DC volume, RF conductivity, light scattering and fluorescence characteristics of blood cells passing through a cell-interrogation zone. Preferably, the transducer includes an electro-optical flow cell which defines a cell-interrogation zone having a square transverse cross-section measuring approximately 50. times. 50 microns, and having a length, measured in the direction of cell flow, of approximately 65 microns.