Skills:
Determination of structure, function, and dynamics of membrane proteins: Ion Channels. Ion channel structure determination by X-ray crystallography in lipidic cubic phase, Ion channel dynamics determination by EPR spectroscopy, Ion channel functional characterization by patch clamp and reconstitution in lipid bilayers including single channel analysis, noise analysis, kinetic modeling. Membrane structure and dynamics by Solid-State NMR spectroscopy, Molecular dynamics simulations of ion channels in lipid bilayers in YASARA, Determination of ligand-channel complex stability by thermal denaturation coupled to fluorescence spectroscopy, Protein Molecular Spectroscopy Techniques: Mass spectroscopy (TOF), Circular dichroism, UV/Vis,Fluorescence (FRET), EPR Molecular Biology and Biochemical Techniques: Cloning, expression, and purification of cytosolic as well as membrane proteins in E.coli, Cloning, protein expression, and functional characterization of eukaryotic protein ion channels in mammalian cell lines including all required cell culture techniques, Expression and purification of bacterial and eukaryotic (human) protein ion channels produced by cell free transcription and translation (IVTT). Production and purification of synthetic antibody fragments (FAB) for chaperone assisted membrane protein crystallography through restricted amino acid diversity antibody phage display. Cloning, protein engineering, expression, purification and characterization utilizing HPLC, FPLC (SEC), Dynamic light scattering, affinity purification, immunocytochemistry, PCR, CODEHOP PCR, Mutagenesis, PCR, and others.