Joseph M Reese

US Patent:

58470778, Dec 8, 1998

Filed:

Sep 19, 1994

Appl. No.:

8/308818

Inventors:

Michael R. Green - Boylston MA
Joseph C. Reese - Shrewsbury MA

Assignee:

University of Massachusetts Medical Center - Worcester MA

International Classification:

C07K 1439
C07K 406
C07K 704
C07K 1437

US Classification:

530350

Abstract:

The present invention provides a complex of fungal polypeptides, termed TAFs, that are necessary for activated transcription in fungi such as S. cerevisiae. The complex comprises at least nine associated subunit polypeptides, having molecular masses of about 180 kDa, 145 kDa, 116 kDa, 90 kDa, 68 kDa, 51-54 kDa, 47 kDa, and 30 kDa, respectively. TAF-145, having the sequence set forth in FIG. 6B, binds TATA-box Binding Protein (TBP). The invention also includes nucleic acid sequences encoding TAF-145, as well as DNA vectors and transformed cells suitable for recombinant expression of this polypeptide.