Joshua T Lang

US Patent:

20140065622, Mar 6, 2014

Filed:

Mar 15, 2013

Appl. No.:

13/832860

Inventors:

Joshua M. Lang - Madison WI, US
Benjamin P. Casavant - Madison WI, US
Scott M. Berry - Madison WI, US
Lindsay N. Strotman - Madison WI, US

International Classification:

G01N 1/40

US Classification:

435 612, 435 30, 4352872, 4352871, 422527

Abstract:

A device and a method are provided for isolating a fraction in a biological sample. The fraction is bound to solid phase substrate to define a fraction-bound solid phase substrate. The device includes an input zone for receiving the biological sample therein to capture a desired fraction of the biological sample. A force is provided that is generally perpendicular to gravity. The force is movable between a first position adjacent the input zone multiple other positions adjacent various purification, protein analysis, separation and extraction zones. The force captures the fraction-bound solid phase substrate and the fraction-bound solid phase substrate moves from the input zone to the other zones to perform a multi-step assay on the isolated fraction within the device.

US Patent:

20200406260, Dec 31, 2020

Filed:

Jun 26, 2020

Appl. No.:

16/912986

Inventors:

- Madison WI, US
Joshua M. Lang - Madison WI, US
Duane S. Juang - Madison WI, US
Scott M. Berry - Madison WI, US
Tamara S. Rodems - Madison WI, US

International Classification:

B01L 3/00
G01N 33/543

Abstract:

A method of sample preparation for streamlined multi-step assays is provided. The method includes the step of providing a microfluidic device including a reservoir defined by a surface configured to repel an aqueous solution. A dried reagent is provided on a portion of the surface and the reservoir is filled with an oil. A first droplet formed from the aqueous solution is positioned on the dried reagent so to pick-up and re-dissolve the dried reagent therein so as to expose the portion of the surface. In addition, a second droplet of an aqueous solution may be deposited on a hydrophilic spot patterned on the surface. A magnetic force may be configured to interact magnetically with the paramagnetic beads within the first droplet to move the droplet through the oil in the reservoir or to move the paramagnetic beads from the first droplet, through the oil, into the second droplet.