Embodiments of the present disclosure provide for devices and methods that can be used in a process for the fast photochemical oxidation of proteins, and the like.
Radical Dosimetry For Analysis Of Biopharmaceuticals And Biological Molecules
- Montara CA, US Joshua S. Sharp - Oxford MS, US Sandeep Misra - Oxford MS, US
International Classification:
G01N 33/68 G01N 21/33 G01N 21/64
Abstract:
The three-dimensional structural analysis of pharmaceutical and/or biological molecules is performed by the reaction of OH radicals on the surfaces of the molecules of interest. Quantitation and/or completeness of the OH radicals are optionally measured using buffers intrinsic to the sample solutions as internal standards. Measurements of the reactions of these buffers with OH radicals provide an internal standard while avoiding the use of prior art internal standards that can have unwanted effects on the three-dimensional structures of interest.
Radical Dosimetry Methods For In Vivo Hydroxyl Radical Protein Foot-Printing
- Montara CA, US Ronald Carl Orlando - Athens GA, US Joshua Shane Sharp - Oxford MS, US Robert Wallace Egan - Reno NV, US Jeffrey Jonathan Persoff - San Jose CA, US
Flash Photo-Oxidation Device and Higher Order Structural Analysis is employed for higher order structural analysis of biomolecules. Biomolecular higher order structure (HOS) results from the confounded superimposition of a biomolecule's secondary, tertiary, and quaternary structure and defines the manner in which a biomolecule presents itself and interacts with other biomolecules in living systems. A rapidly growing class of therapeutic drugs, known as biotherapeutics, comprises a variety of proteins, whose therapeutic properties are inherently linked and dependent upon their HOS. As such, HOS analysis of biotherapeutics is an important analytical requirement in the biopharmaceutical industry. The present invention provides new means and methods for the determination of biopharmaceutical HOS and associated conformation using improved devices and methodologies for flash photo-oxidation of proteins to determine their higher order biomolecular structure, and such is responsive to the increased demand for new and improved HOS analytical means in the biopharmaceutical industry.
Flash Photo-Oxidation Device And Higher Order Structural Analysis
- Montara CA, US Ronald Carl ORLANDO - Athens GA, US Joshua Shane SHARP - Oxford MS, US Robert Wallace EGAN - Reno NV, US Jeffrey Jonathan PERSOFF - San Jose CA, US
Flash Photo-Oxidation Device and Higher Order Structural Analysis is employed for higher order structural analysis of biomolecules. Biomolecular higher order structure (HOS) results from the confounded superimposition of a biomolecule's secondary, tertiary, and quaternary structure and defines the manner in which a biomolecule presents itself and interacts with other biomolecules in living systems. A rapidly growing class of therapeutic drugs, known as biotherapeutics, comprises a variety of proteins, whose therapeutic properties are inherently linked and dependent upon their HOS. As such, HOS analysis of biotherapeutics is an important analytical requirement in the biopharmaceutical industry. The present invention provides new means and methods for the determination of biopharmaceutical HOS and associated conformation using improved devices and methodologies for flash photo-oxidation of proteins to determine their higher order biomolecular structure, and such is responsive to the increased demand for new and improved HOS analytical means in the biopharmaceutical industry.
University of Mississippi
Associate Professor of Pharmacology
University of Mississippi Jul 2015 - Jun 2019
Assistant Professor of Pharmacology
Photochem Technologies Oct 2012 - Jul 2017
Chief Executive Officer
Gennext Technologies Oct 2012 - Jul 2017
Chief Technology Officer
University of Georgia Oct 2012 - Jul 2017
Associate Professor of Pharmacology
Education:
Oak Ridge National Laboratory/The University of Tennessee 2000 - 2003
Doctorates, Doctor of Philosophy, Philosophy
University of Tennessee, Knoxville 1995 - 1999
Bachelors, Bachelor of Science, Microbiology
Skills:
Protein Chemistry Biochemistry Mass Spectrometry Proteomics Protein Purification Molecular Biology Chemistry Chromatography Analytical Chemistry Biotechnology Bioinformatics Hplc Lc Ms Western Blotting High Performance Liquid Chromatography
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